ESR 6

Top-down paleoproteomics

ESR6: Top-down paleoproteomics. (@ UCPH, supervisor JVO).

As an alternative to bottom-up proteomics, the emergence of new ion dissociation methods continues to drive top-down proteomics by offering valuable alternatives to traditional slow-heating methods (e.g., collision-activated dissociation, CAD). Electron transfer dissociation (ETD) leverages electron-driven radical rearrangements to promote cleavage of N–Cα bonds between amino acid residues, preserving labile post-translational modifications (PTMs) and providing extensive sequence-informative fragmentation of peptides and proteins. The complementary of the different types of fragmentation methods significantly increases confidence in peptide and protein identification in particular for PTM analyses. Therefore, top-down analysis allows for the measurement of intact protein masses and provides information on post-translational modifications, as well as the protein sequence via fragmentation of the intact proteins in the mass spectrometer. Application of top-down proteomics to cultural heritage materials will remove many of the limits hampering analysis of this category of samples.

Networking. Within TEMPERA, ESR 6 will interact primarily with:

  1. ESR 7: ESR 6 will spend a secondment period of 6 months at MPI (co-supervision) to collaborate with ESR 7 and colleagues for automatic interpretation of the data he/she will generate using the methodologies he/she will develop.
  2. ESR 8: ESR 6 will spend a secondment period of 6 months at Thermo (Bremen) to work on hardware improvement in a company environment.