Personal Academic Website

BIBLIOGRAPHICAL INFORMATION

Curriculum Vitae

Born in Hradec Kralove, Czech Republic

Citizenship: Czech Republic

Education & Academic Qualifications:

2006:               DSc. in Zoology and Animal Physiology, Academy of Sciences of the Czech Republic

(Dissertation: “Physiological regulations of transmembrane signaling systems mediated by trimeric G-proteins”)

1991 - 1995: Gothenburg University, Faculty of Medicine, Gothenburg, Sweden

1996:           Ph.D. in Physiology, Academy of Sciences of the Czech Republic

(Dissertation: “Studies on the G protein-mediated adenylyl cyclase signalling during hormonal stimulation and viral infection”)

1978 - 1983: Charles University in Prague, Faculty of Science, Prague, Czech Republic

1983:               M.S. in Analytical Chemistry (Diploma thesis: “A study of spectrophotometric characteristics of phenothiazine and its derivatives”)

1983:               RNDr. (Rerum Naturalium Doctoris) degree in Analytical Chemistry

Principal Appointments & Work Abroad Experience:

09/2007 - present: Charles University in Prague, Faculty of Science, Department of Physiology, Prague, Czech Republic

  Associate Professor, Head of the Department of Physiology

08/2003 - 09/2003: University of Glasgow, Institute of Biomedical and Life Sciences, Glasgow, United Kingdom

  Visiting Scientist

06/1998 - 08/1998: University of Glasgow, Institute of Biomedical and Life Sciences, Glasgow, United Kingdom

                                   Visiting Scientist

08/1996 - 08/2007: Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic

Senior Scientist, Deputy Head of the Department of Biochemistry of Membrane-Bound Receptors

01/1996 - 07/1996: SEVAC (formerly Institute of Sera and Vaccines), Department of Virology, Prague, Czech Republic

  Research Fellow, Deputy Head of the Virus Dignostic Laboratory

1991 - 1995: Gothenburg University, Faculty of Medicine, Department of Clinical Chemistry and Transfusion Medicine, and Wallenberg Laboratory for Cardiovascular       Research, Gothenburg, Sweden

   Postgraduate Fellow

1985 - 1991: Institute of Sera and Vaccines, Department of Virology, Prague, Czech Republic

   Research Fellow, Deputy Head of the Virus Dignostic Laboratory

1983 - 1985: Charles University in Prague, Faculty of Science, Department of Plant Physiology and Soil Microbiology

   Junior Research Fellow

Professional Affiliations:    Czech Society for Physiology

Czech Society for Biochemistry and Molecular Biology

Czech Society for Neuropsychopharmacology

RESEARCH INTERESTS

My research interests lie in the following areas:

·  Structural and functional aspects of transmembrane signaling regulated by GPCRs and trimeric G proteins.

·  Mechanisms of potential cardioprotective effects of opioids, chronic hypoxia, and cold aclimation.

·  Drugs of abuse – mechanism of drug tolerance and dependence; oxidative stress, apoptosis and potential cytoprotective effects of drugs.

·  Neurodegeneration, senescence, and neurotransmitter systems..

·  Modulation of circadian clock function.

PUBLICATIONS

Peer-Reviewed Journal Articles

Nemcova I., Novotny J. and Horska V. (1986) Spectrophotometric study of phenothiazine derivatives and their cation radicals in micelar media. Microchem. J. 34:180-189.

Nemcova I., Masojidkova M. and Novotny J. (1988) An NMR study of the interaction of phenothiazine derivatives with cationic and anionic tensides. Microchem. J. 38:80-85.

Novotny J. (1990) Properties and use of mumps virus antigen for detection of specific IgG and IgM antibodies in enzyme-linked immunosorbent assay. Acta Virol. 34:574-577.

Novotny J., Arenberger P. and Matouskova E. (1991) Cultivation of psoriatic keratinocytes and an attempt to isolate and detect a specific psoriatic antigen. Folia Biol. 37:52-54.

Fedova D., Novotny J. and Kubinova I. (1992) Serological diagnosis of parainfluenza virus infections. Verification of the sensitivity and specifity of the hemagglutination-inhibition (HI), complement-fixation (CF), immunofluorescence (IFA) tests and enzyme immunoassay (ELISA). Acta Virol. 36:304-312.

Novotny J., Svobodova J., Ransnäs L.A. and Kubistova K. (1992) A method for the preparation of purified antigens of coxsackievirus B3 from a large volume of cell culture supernatant. Acta Virol. 36:483-487.

Kvapil P., Novotny J. and Ransnäs L.A. (1994) Activated Gsa but not Gia prevents the thermal inactivation of adenylyl cyclase in plasma membranes derived from S49 lymphoma cells. FEBS Lett. 343:208-212.

Novotny J., Gustafson B., Kvapil P. and Ransnäs L.A. (1994) Adenovirus infection of myocardial cells induces an enhanced sensitivity to b-adrenergic agonists by increasing the concentration of the stimulatory G-protein. Biochem. Mol. Biol. Int. 34:993-1001.

Novotny J., Kvapil P., Svobodova J., Cello J. and Ransnäs L.A. (1994) Coxsackie B3 virus penetration into rat cardiocytes alters the adenylyl cyclase signalling system. Biomed. Res. 15:114-122.

Novotny, J., Kvapil, P., Cello, J. and Ransnäs, L.A. (1994) Coxsackievirus B3 entry into the host cell interferes with G-protein-mediated transmembrane signalling. Biosci. Rep. 14:205-214.

Kvapil P., Novotny J., Svoboda P. and Ransnäs L.A. (1994) The short and long forms of the a subunit of the stimulatory guanine nucleotide-binding  protein  are unequally  redistributed  during (-)-isoproterenol mediated desensitization of intact S49 lymphoma cells. Eur. J. Biochem. 226:193-199.

Novotny J., Kvapil P., Bokoch G.M. and Ransnäs L.A. (1995) Isoproterenol-induced subcellular redistribution of G-protein b subunits in S49 lymphoma cells demonstrated by a novel competitive ELISA. Arch. Physiol. Biochem. 103:202-210.

Kvapil P., Novotny J. and Ransnäs L.A. (1995) Prolonged exposure of hamsters to cold changes the levels of G proteins in brown adipose tissue plasma membranes. Life Sci. 57:311-318.

Novotny J., Kvapil P., Jelinek F. and Ransnäs L.A. (1995) Alterations in G-protein-regulated transmembrane signalling induced in murine myocardium by coxsackievirus B3 infection. Cardiovasc. Res. 30:602-610.

Novotny J., Gustafson G. and Ransnäs L.A. (1996) Inhibition of b-adrenergic receptor-mediated signals by a synthetic peptide derived from the a subunit of the stimulatory G-protein. Biochem. Biophys. Res. Commun. 219:619-624.         

Novotny J. and Svoboda, P. (1998) The long (Gsa-L) and short  (Gsa-S) variants of the stimulatory guanine nucleotide-binding protein. Do they behave in an identical way? J. Mol. Endocrinol. 20:163-173.

Drmota T., Novotny J., Kim G.-D., Eidne K.A., Milligan G. and Svoboda P. (1998) Agonist-induced internalization of the G protein G11a and thyrotropin-releasing hormone receptors proceed on different time scales. J. Biol. Chem. 273:21699-21707.

Schonning K., Bolmstedt A., Novotny J., Lund O.S., Olofsson S. and Hansen, J.-E.S. (1998) Induction of antibodies against epitopes inaccessible on the HIV type 1 envelope oligomer by immunization with recombinant monomeric glycoprotein 120. AIDS Res. Hum. Retrov. 14:1451-1456.

Bourova L, Novotny J. and Svoboda P. (1999) The decrease in the short variant of Gsa protein is associated with an increase in [3H]CGP 12177 binding, [3H]ouabain binding and Na,K-ATPase activity in brown adipose tissue plasma membranes of cold- acclimated hamsters. J. Mol. Endocrinol. 22:55-64.

Novotny J., Bourova L., Malkova O., Svoboda P. and Kolar F. (1999) G proteins, b-adrenoceptors and b-adrenergic responsiveness in immature and adult rat ventricular myocardium: influence of neonatal hypo- and hyperthyroidism. J. Mol. Cell. Cardiol. 31:761-772.

Novotny J., Krusek J., Drmota T. and Svoboda P. (1999) Overexpression of the G protein G11a prevents desensitization of Ca2+ response to thyrotropin-releasing hormone. Life Sci. 65:889-900.

Drmota T., Novotny J., Gould G.W., Svoboda P. and Milligan G. (1999) Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and Gqa/G11a induced by agonist stimulation. Biochem. J. 340:529-538.                                                                            

Bourova L., Novotny J. and Svoboda P. (1999) Resolution and identification of Gq/G11a and Gia/Goa proteins in brown adipose tissue: effect of cold acclimation. J. Mol. Endocrinol. 23:223-229.                                                                   

Pesanova Z., Novotny J., Cerny J., Milligan G. and Svoboda P. (1999) Thyrotropin-releasing hormone-induced depletion of Gqa/G11a proteins from detergent-insensitive membrane domains. FEBS Lett. 464:35-40.

Kalmusova J., Novotny J., Hulinska D., Musilek M. and Kriz P. (2000) Interactions of invasive and noninvasive strains of Neisseria meningitidis with monkey epithelial cells, mouse monocytes and human macrophages. Microbiologica 23:185-200.

Bourova L., Pesanova Z., Novotny J., Bengtsson T. and Svoboda P. (2000) Differentiation of cultured brown adipocytes is associated with a selective increase in the short variant of Gsa protein. Evidence for higher functional activity of GsaS. Mol. Cell. Endocrinol. 167:23-31.

Novotny J., Bourova, L., Kolar, F. and Svoboda P. (2001) Membrane-bound and cytosolic forms of heterotrimeric G proteins in young and adult rat myocardium: influence of neonatal hypo- and hyperthyroidism. J. Cell. Biochem. 82:215-224.

Ihnatovych I., Hejnova L., Kostrnova A., Mares P., Svoboda P. and Novotny J. (2001) Maturation of rat brain is accompanied by differential expression of the long and short splice variants of Gsa protein. Identification of cytosolic forms of Gsa. J. Neurochem. 79:88-97.

Svoboda P. and Novotny J. (2002) Hormone-induced subcellular redistribution of trimeric G proteins. Cell. Mol. Life Sci. 59:501-512.

Ihnatovych I., Novotny J., Haugvicova R., Bourova L., Mares P. and Svoboda P. (2002) Opposing changes of trimeric G proteins levels during ontogenetic development of rat brain. Dev. Brain Res. 133:57-67.

Ihnatovych I., Novotny J., Haugvicova R., Bourova L., Mares P. and Svoboda P. (2002) Ontogenetic development of the G protein-mediated adenylyl cyclase signalling in rat brain. Dev. Brain Res. 133:69-75.

Flachs P., Novotny J., Baumruk F., Bardova K., Bourova L., Miksik I., Svoboda P., Sponarova J. and Kopecky J. (2002) Impaired norepinephrine-induced lipolysis in white fat of a P2-Ucp1 transgenic mice is associated with changes in G-protein levels. Biochem. J. 364:369-376.

Hejnova L., Ihnatovych I., Novotny J., Kubova H., Mares P. and Svoboda P. (2002) Modulation of adenylyl cyclase activity by baclofen in the developing rat brain: difference between cortex, thalamus and hippocampus. Neurosci. Lett. 330:9-12.

Novotny J., Hrbasova M., Kolar F. and Svoboda P. (2003) Cardiomegaly induced by pressure overload in newborn rats is accompanied by altered expression of the long isoform of Gsa protein and deranged signaling of adenylyl cyclase. Mol. Cell. Biochem. 245:157-166.

Bourova L., Kostrnova A., Hejnova L., Moravcova Z., Moon H.E., Novotny J., Milligan G. and Svoboda P. (2003) -Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains. J. Neurochem. 85:34-49.

Hrbasova M., Novotny J., Hejnova L., Kolar F., Neckar J. and Svoboda P. (2003) Altered myocardial Gs protein and adenylyl cyclase signaling in rats exposed to chronic hypoxia and normoxic recovery. J. Appl. Physiol.  94:2423-2432.

Matousek P., Novotny J. and Svoboda P. (2004) Resolution of Gs and Gq/G11 a proteins in membrane domains by two-dimensional electrophoresis. The effect of long-term agonist stimulation. Physiol. Res. 53:295-303.

Moravcova Z., Rudajev V., Stöhr J., Novotny J., Cerny J., Patrenti M., Milligan G. and Svoboda P. (2004) Long-term agonist stimulation of IP prostanoid receptor depletes the cognate Gsa protein in membrane domains but does not change the receptor level. Biochim. Biophys. Acta 1691:51-65.

Stöhr J., Bourova L., Hejnova L., Ihnatovych I., Novotny J. and Svoboda P. (2004) Increased baclofen-stimulated G protein coupling and deactivation in rat brain cortex during development. Dev. Brain Res. 151:67-73.

Svoboda P., Teisinger J., Novotny J., Bourova L., Drmota T., Hejnova L., Moravcova Z., Lisy V., Rudajev V., Stöhr J., Vokurkova A., Svandova I. and Durchankova D. (2004) Biochemistry of transmembrane signaling mediated by trimeric G proteins. Physiol. Res. 53:S141-S152.

Matousek P., Novotny J., Rudajev, V. and Svoboda P. (2005) Prolonged agonist stimulation does not alter the protein composition of membrane domains in spite of dramatic changes induced in a specific signaling cascade. Cell Biochem. Biophys. 42:21-40.

Stöhr J., Novotny J., Bourova L. and Svoboda P. (2005) Modulation of adenylyl cyclase activity in young and adult rat brain cortex. Identification of suramin as a direct inhibitor of adenylyl cyclase. J. Cell. Mol. Med. 9:940-952.

Stöhr J., Novotny J. and Svoboda P. (2005) Characterization of [3H]-forskolin binding sites in young and adult rat brain cortex. Identification of suramin as a competitive inhibitor of [3H]-forskolin binding. Can. J. Physiol. Pharmacol. 83:573-581.

Rudajev V., Novotny J., Hejnova L., Milligan G. and Svoboda P. (2005) Thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and Gqa/G11a protein. J. Biochem. 138:111-125.

Matousek P., Durchankova D., Svandova I., Novotny J. and Svoboda P. (2005) Agonist-induced tyrosin phosphorylation of Gq/G11a requires the intact structure of membrane domains. Biochem. Biophys. Res. Commun. 328:526-532.

Novotny J., Durchankova D., Ward R.J., Carrillo J.J., Svoboda P. and Milligan G. (2006) Functional interactions between the a1b-adrenoceptor and Ga11 are compromised by de-palmitoylation of the G protein but not of the receptor. Cell. Signal. 17:1244-1251.

Ostasov P., Bourova L., Hejnova L, Novotny J. and Svoboda P. (2007) Disruption of the plasma membrane integrity by cholesterol depletion impairs effectiveness of TRH receptor-mediated signal transduction via Gq/G11a proteins. J. Recept. Signal Transduct. 27:335-352.

Ostasov P., Krusek J., Durchankova D., Svoboda P. and Novotny J. (2008) Ca2+ responses to thyrotropin-releasing hormone and angiotensin II. The role of plasma membrane integrity and effect of G11a  protein overexpression in homologous and heterologous desensitization. Cell. Biochem. Funct. 26:264-274.

Durchankova D., Novotny J. and Svoboda P. (2008) The time-course of agonist-induced solubilization of trimeric Gqa/G11a proteins resolved by two-dimensional electrophoresis. Physiol. Res. 57:195-203.

Drastichova Z., Bourova L., Lisy V., Hejnova L., Rudajev V., Stöhr J., Durchankova D., Ostasov P., Teisinger J., Soukup T., Novotny J., Svoboda P. (2008) Subcellular redistribution of trimeric G-proteins – potential mechanism of desensitization of hormone response: internalization, solubilization, down-regulation. Physiol. Res. 57(Suppl. 3):S1-10.

Bourova L., Stöhr J., Lisy V., Rudajev V., Novotny J. and Svoboda P. (2009) Isolation of plasma membrane compartments from rat brain cortex; detection of agonist-stimulated G protein activity. Med. Sci. Monit. 15:BR111-122.

Drastichova Z., Bourova L., Hejnova L., Jedelsky P., Svoboda P. and Novotny J. (2010) Protein alterations induced by long-term agonist treatment of HEK293 cells expressing thyrotropin-releasing hormone receptor and G11α protein. J. Cell. Biochem. 109:255-264.

Bourova L., Vosahlikova M., Kagan D., Dlouha K., Novotny J. and Svoboda P. (2010) Long-term adaptation to high doses of morphine causes desensitization of mu-OR- and delta-OR-stimulated G-protein response in forebrain cortex but does not decrease the amount of G-protein alpha subunits. Med. Sci. Monit. 16:BR260-270.

Drastichova Z., Skrabalova J., Neckar J., Kolar F. and Novotny J. (2011) Prolonged morphine administration alters protein expression in the rat myocardium. J. Biomed. Sci. 18:89-97.

Skrabalova J., Neckar J., Hejnova L., Bartonova I., Kolar F. and Novotny J. (2012) Antiarrhytmic effect of prolonged morphine exposure is accompanied by altered myocardial adenylyl cyclase signaling in rats. Pharmacol. Rep. 64:351-359.

Drastichova Z. and Novotny J. (2012) Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells. Acta Biochim. Biophys. Sin. 44:641-649.

Drastichova Z., Skrabalova J., Jedelsky P., Neckar J., Kolar F. and Novotny J. (2012) Global changes in the rat heart proteome induced by prolonged morphine treatment and withdrawal. PLoS One 7:e47167.

Waskova-Arnostova P., Elsnicova B., Kasparova D., Sebesta O., Novotny J., Neckar J., Kolar F. and Zurmanova J. (2013) Right-to-left ventricular differences in the expression of mitochondrial hexokinase and phosphorylation of Akt. Cell. Physiol. Biochem. 31:66-79.

Kockova R., Svatunkova J., Novotny J., Hejnova L., Ostadal B. and Sedmera D. (2013) Heart rate changes mediate the embryotoxic effect of antiarrhythmic drugs in the chick embryo. Am. J. Physiol. Heart Circ. Physiol. 304:H895-H902.

Klevstig M., Manakov D., Kasparova D., Brabcova I., Papousek F., Zurmanova J., Zidek V., Silhavy J., Neckar J., Pravenec M., Kolar F., Novakova O. and Novotny J. (2013) Transgenic rescue of defective Cd36 enhances myocardial adenylyl cyclase signaling in spontaneously hypertensive rats. Pflugers Arch. Eur. J. Physiol. 465:1477-1486.

Skrabalova J., Drastichova Z. and Novotny J. (2013) Morphine as a potential oxidative stress-causing agent. Mini-Rev. Org. Chem. 10:367-372.

Driak D., Dvorska M., Bolehovska P., Svandova I., Novotny J. and Halaska M. (2014) Bad and Bid – potential background players in preneoplastic to neoplastic shift in human endometrium. Neoplasma 61:411-415.

Ujcikova H., Brejchova J., Vosahlikova M., Kagan D., Dlouha K., Sykora J., Merta L., Drastichova Z., Novotny J., Ostasov P., Roubalova L., Hof M. and Svoboda P. (2014) Opioid-receptor signaling cascades in rat cerebral cortex and model cell lines: the role of plasma membrane structure. Physiol Res. 63(Suppl. 1):S165-176.

Waskova-Arnostova P., Kasparova D., Elsnicova B., Novotny J., Neckar J., Kolar F. and Zurmanova J. (2014) Chronic hypoxia enhances expression and activity of mitochondrial creatine kinase and hexokinase in the rat ventricular myocardium. Cell. Physiol. Biochem. 33:310-320.

Hejnova L., Hahnova K., Kockova R., Svatunkova J., Sedmera D. and Novotny J. (2014) Adenylyl cyclase signaling in the developing chick heart: the deranging effect of antiarrhythmic drugs. Biomed Res. Int. ID 463123.

Pacesova D., Volfova B., Cervena K., Hejnova L., Novotny J. and Bendova Z. (2015) Acute morphine affects the rat circadian clock via rhythms of physphorylated ERK1/2 and GSK3β kinases and Per1 expression in the rat suprachiasmatic nucleus. Br. J. Pharmacol. 172:3638-3649.

Waskova-Arnostova P., Elsnicova B., Kasparova D., Kolar F., Novotny J. and Zurmanova J. (2015) Cardioprotective adaptation of rats to intermittent hypobaric hypoxia is accompanied by the increased association of hexokinase with mitochondria. J. Appl. Physiol. 119:1487-1493.

Kasparova D., Neckar J., Dabrowska L., Novotny J., Mraz J., Kolar F. and Zurmanova J. (2015) Cardioprotective and non-protective regimens of chronic hypoxia diversely affect the myocardial antioxidant systems. Physiol. Genomics. 47:612-620.

Hahnova K., Kasparova D., Neckar J., Zurmanova J., Kolar F. and Novotny J. (2016) β-Adrenergic signaling in rat heart is similarly affected by continuous and intermittent normobaric hypoxia. Gen. Physiol. Biophys. 35:165-173.

Pacesova D., Novotny J. and Bendova Z. (2016) The effect of chronic morphine or methadone exposure and withdrawal on clock gene expression in the rat suprachiasmatic nucleus and AA-NAT activity in the pineal gland. Physiol Res. 65:517-525.

Manakov D., Ujcikova H., Pravenec M. and Novotny J. (2016) Alterations in the cardiac proteome of the spontaneously hypertensive rat induced by transgenic expression of CD36. J. Proteomics 145:177-186.

Melkes B., Hejnova L. and Novotny J. (2016) Biased μ-opioid receptor agonists diversely regulate lateral mobility and functional coupling of the receptor to its cognate G proteins. Naunyn-Schmiedeberg´s Arch. Pharmacol. 389:1289-1300.

Micova P., Hahnova K., Hlavackova M., Elsnicova B., Chytilova A., Holzerova K., Zurmanova J., Neckar J., Kolar F., Novakova O. and Novotny J. (2016) Chronic intermittent hypoxia affects the cytosolic phospholipase A2α/cyclooxygenase 2 pathway via β2-adrenoceptor-mediated ERK/p38 stimulation. Mol. Cell. Biochem. 423:151-163.

Micova P., Holzerova K., Vecka M., Zurmanova J., Neckar J., Kolar F., Novakova O., Novotny J. and Hlavackova M (2017) Antioxidant tempol supresses heart cytosolic phospholipase A2α stimulated by chronic intermittent hypoxia. Can. J. Physiol. Pharmacol. 95:920-927.

Neckar J. Chytilova A., Weissova R., Drahota Z., Zajickova P., Brabcova I., Kolar D., Alanova P., Vasinova J., Silhavy J., Hlavackova M., Tauchmannova K., Milerova M., Ostadal B.,  Cervenka L., Zurmanova J., Kalous M, Novakova O., Novotny J., Pravenec M. and Kolar F. (2017) Selective replacement of mitochondrial DNA increases cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin. Sci. 131:865-881.

Kolar D., Gresikova M., Waskova-Arnostova P., Elsnicova B., Kohutova J., Hornikova D., Vebr P., Neckar J., Blahova T., Kasparova D., Novotny J., Kolar F., Novakova O. and Zurmanova J. (2017) Adaptation to chronic continuous hypoxia potentiates Akt/HK2 anti-apoptotic pathway during brief myocardial ischemia/reperfusion insult. Mol. Cell. Biochem. 432:99-108.

Hejnova L., Skrabalova J. and Novotny J. (2017) Prolonged morphine treatment alters expression and plasma membrane distribution of β-adrenergic receptors and some other components of their signaling system in rat cerebral cortex. J. Mol. Neurosci. 63:364-376.

Skrabalova J., Karlovska I., Hejnova L. and Novotny J. (2018) Protective effect of morphine against the oxidant-induced injury in H9c2 cells. Cardiovasc. Toxicol. 18:374-385.

Hahnova K., Brabcova I., Neckar J., Weissova R., Svatonova A., Novakova O., Zurmanova J., Kalous M., Silhavy J., Pravenec M., Kolar F. and Novotny J. (2018) β-Adrenergic signaling, monoamine oxidase A and antioxidant defence in the myocardium of SHR and SHR-mtBN conplastic rat strains: the effect of chronic hypoxia. J. Physiol. Sci. 68:441-454.

Bolehovska P., Driak D., Halaska M., Asfaw B., Novotny J. and Svandova I. (2018) Disturbed angiogenesis in IUGR-compromised placentas at term. Clin. Exp. Obstet. Gynecol. 45:707-712.

Moravcova R., Melkes B. and Novotny J. (2018) TRH receptor mobility in the plasma membrane is strongly affected by agonist binding and by interaction with some cognate signaling proteins. J. Recept. Signal Transduct. 38:20-25.

Nedvedova I., Kolar D., Elsnicova B., Hornikova D., Novotny J., Kalous M., Pravenec M., Neckar J., Kolar F. and Zurmanova J.M. (2018) Mitochondrial genome modulates myocardial Akt/Glut/HK salvage pathway in spontaneously hypertensive rats adapted to chronic hypoxia. Physiol. Genomics 50:532-541.

Manakov D., Kolar D., Zurmanova J., Pravenec M. and Novotny J. (2018) Changes in the activity of some metabolic enzymes in the heart of SHR rat incurred by transgenic expression of CD36. J. Physiol. Biochem. 74:479-489.

Moravcova S., Pacesova D., Melkes B., Kyclerova H., Spisska V., Novotny J. and Bendova Z. (2018) The day/night difference in the circadian clock's response to acute lipopolysaccharide and the rhythmic Stat3 expression in the rat suprachiasmatic nucleus. PLoS One 13:e0199405.

Filipovska E., Cervena K., Moravcova S., Novotny J., Kyclerova H., Spisska V., Pacesova D. and Bendova Z. (2019) The effect of the cannabinoid receptor agonist and antagonist on the light-induced changes in the suprachiasmatic nucleus of rats. Neurosci. Lett. 703:49-52.

Cockova Z., Ujcikova H., Telensky P. and Novotny J. (2019) Protein profiling of SH-SY5Y neuroblastoma cells: the effect of rhein. J. Biosci. 44:88.

Bendova Z., Pacesova D. and Novotny J. (2020) The day-night differences in ERK1/2, GSK3β activity and c-Fos levels in the brain, and the responsiveness of various brain structures to morphine. J. Comp. Neurol. 528:2471-2495.

Melkes B., Markova V., Hejnova L., Marek A. and Novotny (2020) Naloxone is a potential binding ligand and activator of the capsaicin receptor TRPV1. Biol. Pharm. Bull. 43:908–912.

Tibenska V., Benesova A., Vebr P., Liptakova A., Hejnova L., Elsnicova B., Drahota Z., Hornikova D., Galatik F., Kolar D., Vybiral S., Alanova P., Novotny J., Kolar F., Novakova O. and Zurmanova J.M. (2020) Gradual cold acclimation induces cardioprotection without affecting β-adrenergic receptor-mediated adenylyl cyclase signaling. J. Appl. Physiol. (1985) 128:1023-1032.

Melkes B., Markova V., Hejnova L. and Novotny J. (2020) β-Arrestin 2 and ERK1/2 are important mediators engaged in close cooperation between TRPV1 and µ-opioid receptors in the plasma membrane. Int. J. Mol. Sci. 21:4626.

Rudajev V. and Novotny J. (2020) The role of lipid environment in ganglioside GM1-induced amyloid β aggregation. Membranes 10:226.

Spisska V., Pacesova D., Mikova H., Pohanova P., Telensky P., Novotny J. and Bendova Z. (2020) Prenatal exposure to lipopolysaccharide induces changes in the circadian clock in the SCN and AA-NAT activity in the pineal gland. Brain Res. 1743:146952.

Silhavy J., Malinska H., Hüttl M., Markova I., Oliyarnyk O., Mlejnek P., Simakova M., Liska F., Kazdova L., Moravcova R., Novotny J. and Pravenec M. (2020) Downregulation of the Glo1 gene is associated with reduced adiposity and ectopic fat accumulation in spontaneously hypertensive rats. Antioxidants 9:1179.

Moravcova S., Cervena K., Mikova H., Pacesova D., Pallag G., Novotny J. and Bendova Z. (2021) Social defeat stress affects resident´s clock gene and Bdnf expression in the brain. Stress 24:206-212.

Tibenska V., Marvanova A., Elsnicova B., Hejnova L., Vebr P., Novotny J., Kolar F., Novakova O. and Zurmanova J.M. (2021) The cardioprotective effect persisting during recovery from cold acclimation is mediated by the β2-adrenoceptor pathway and Akt activation. J. Appl. Physiol. (1985) 130:746-755.

Honetschlagerova Z., Gawrys O., Jíchova S., Skaroupkova P., Kikerlova S., Vanourkova Z., Huskova Z., Melenovsky V., Kompanowska A., Sadowsky J., Kolar F., Novotny J., Hejnova L., Kujal P. and Cervenka L. (2021) Renal sympathetic denervation attenuates congestive heart failure in angiotensin II- dependent hypertension: studies with Ren-2 transgenic hypertensive rats with aorto-caval fistula. Kidney Blood Press. Res. 46:95-113.

Drastichova Z., Pallag G., Rudajev V. and Novotny J. (2021) Proteome profiling of different rat brain regions reveals the modulatory effect of prolonged maternal separation on proteins involved in cell death-related processes. Biol. Res. 54:4.

Ujcikova H., Hejnova L., Eckhardt A., Roubalova L., Novotny J. and Svoboda P. (2021) Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies. Neurochem. Int. 151:104975.

Cockova Z., Honc O., Telensky P.. Olsen M. and Novotny J. (2021) Streptozotocin-induced astrocyte mitochondrial dysfunction is ameliorated by FTO inhibitor MO-I-500. ACS Chem. Neurosci. 12:3818-3828.

Drastichova Z., Hejnova L., Moravcova R. and Novotny J. (2021) Proteomic analysis unveils expressional changes in cytoskeleton- and synaptic plasticity-associated proteins in rat brain six months after withdrawal from morphine. Life 11:683.

Neckar J., Alanova P., Olejnickova V., Papousek F., Hejnova L., Silhavy J., Behuliak M., Bencze M., Hrdlicka J., Vecka M., Jarkovska D., Sviglerova J., Mistrova E., Stengl M., Novotny J., Ostadal B., Pravenec M. and Kolar F. (2021) Excess ischemic tachyarrhythmias trigger protection against myocardial infarction in hypertensive rats. Clin. Sci. 135:2143-2163.

Pacesova D., Spisska V., Novotny J. and Bendova Z. (2021) Maternal morphine intake during pregnancy and lactation affects the circadian clock of the rat pups. Brain Res. Bull. 177:143-154.

Markova V., Hejnova L., Benda A., Novotny J. and Melkes B. (2021) β-Arrestin 1 and 2 similarly influence m-opioid receptor mobility and distinctly modulate adenylyl cyclase activity. Cell. Signal. 87:110124.

Moravcova S., Filipovska E., Spisska V., Svobodova I., Novotny J. and Bendova Z. (2021) The circadian rhythms of STAT3 in the rat pineal gland and its involvement in the arylalkylamine-N-acetyltransferase regulation. Life 11:1105.

Honetschlagerova Z., Hejnova L., Novotny J., Marek A. and Cervenka L. (2021) Effects of renal denervation on the enhanced renal vascular responsiveness to angiotensin II in high-output heart failure: angiotensin II receptor binding assessment and functional studies in Ren-2 transgenic hypertensive rats. Biomedicines 9:1803.

Pravenec M., Silhavy J., Mlejnek P., Simákova M., Mracek T., Pecinova A., Tauchmannova K., Hütl M., Malinska H., Kazdova L., Neckar J., Kolar F., Zurmanova J., Novotny J. and Houstek J. (2021) Conplastic strains for identification of retrograde effects of mitochondrial DNA variation on cardiometabolic traits in the spontaneously hypertensive rat. Physiol. Res. 70 (Suppl. 4):S471-S484.

Pacesova D., Spisska V., Novotny J. and Bendova Z. (2022) Circadian rhythms of locomotor activity in rats: Data on the effect of morphine administered from the early stages of embryonic development until weaning. Data Brief 40:107812.

Rudajev V. and Novotny J. (2022) Cholesterol as a key player in amyloid β-mediated toxicity in Alzheimer´s disease. Front. Mol. Neurosci. 15:937056.

Ujcikova H., Eckhardt A., Hejnova L., Novotny J. and Svoboda P. (2022) Alterations in the proteome and phosphoproteome profiles of rat hippocampus after six months of morphine withdrawal; comparison with the forebrain cortex. Biomedicines 10:80.

Drastichova Z., Trubacova R. and Novotny J. (2022) β-Arrestin2 is critically involved in the differential regulation of phosphosignaling pathways by thyrotropin-releasing hormone and taltirelin. Cells 11:1473.

Moravcova S., Spisská V., Pacesova D., Hrubcova L., Kubistova A., Novotny J. and Bendova Z. (2022) Circadian control of kynurenine pathway enzymes in the rat pineal gland, liver, and heart and tissue- and enzyme-specific responses to lipopolysaccharide. Arch. Biochem. Biophys. 722:109213.

Mali A.S. and Novotny J. (2022) Opioid receptor activation suppresses the neuroinflammatory response by promoting microglial M2 polarization. Mol. Cell. Neurosci. 121:103744.

Novakova D.,  Kudlacek K., Novotny J. and Nesmerak K. (2022) Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection. Monatsh. Chem. 153:753-758.

Trubacova R., Drastichova Z. and Novotny J. (2022) Biochemical and physiological insights into TRH receptor-mediated signaling. Front. Cell. Dev. Biol. 10:981452.

Sultana P. and Novotny J. (2022) Rab11 and its role in neurodegenerative diseases. ASN Neuro 14:7590914221142360.

Lapka M., Charalambous Ch., Khryakova A., Certilina A., Novotny J., Hejnova L. and Sustkova-Fiserova M. (2023) Ghrelin/GHS-R1A antagonism in memory test and its effects on central molecular signaling involved in addiction in rats. Pharmacol. Biochem. Behav. 224:173528.

Silhavy J., Mlejnek P., Simakova M., Markova I., Malinska H., Hüttl M., Kazdova L., Kazantsev D., Mancini M., Novotny J. and Pravenec M. (2023) CD36 regulates substrates utilisation in brown adipose tissue of spontaneously hypertensive rats: In vitro study. PLoS One 18:e0283276.

Ujcikova H., Hejnova L., Novotny J. and Svoboda P. (2023) Protracted morphine withdrawal induces upregulation of peroxiredoxin II and reduces 14-3-3 protein levels in the rat brain cortex and hippocampus. Brain Res. 1813:148428.

Mali A.S., Honc O., Hejnova L. and Novotny J. (2023) Opioids ameliorate oxidative stress via the Nrf2/HO-1 pathway in LPS-stimulated microglia. Int. J. Mol. Sci. 24:11089.

Honc O. and Novotny J. (2023) Methadone potentiates cytotoxicity of temozolomide via impairing calcium homeostasis and dysregulation of PARP in glioblastoma cells. Cancers 10:15143567.

Pacesova S., Spisska V., Novotny J. and Bendova Z. (2023) Methadone administered to rat dams during pregnancy and lactation affects the circadian clock of their pups. J. Neurosci. Res. 101:1737-1756.

Rudajev V., Novotny J. (2023) Cholesterol-dependent amyloid β production: space for multifarious interactions between amyloid precursor protein, secretases, and cholesterol. Cell. Biosci.13:171.

Marvanova A., Kasik P., Elsnicova B., Tibenska V., Galatik F., Hornikova D., Zvolska V., Vebr P., Vodicka P., Hejnova L., Matous P., Szeiff Bacova B., Sykora M., Novotny J., Neuzil J., Kolar F., Novakova O. and Zurmanova J.M. (2023) Continuous short-term acclimation to moderate cold elicits cardioprotection in rats, and alters β-adrenergic signaling and immune status. Sci. Rep. 13:18287. 

Drastichova Z., Trubacova R. and Novotny J. (2023) Regulation of phosphosignaling pathways involved in transcription of cell cycle target genes by TRH. Biomed. Pharmacol. 168:115830.

TEACHING AND SUPERVISION OF STUDENT PROJECTS 

 I am currently participating in teaching the following courses:

·  Animal and Human Physiology

·  Physiology of Stress

·  Neurobiology

·  Neurochemistry

·  Molecular Pharmacology

·  Behavioral Pharmacology

·  Toxicology

·  Experimental Techniques in Neuroscience

·  Seminars in Animal and Human Physiology

·  Advances in Physiology and Neuroscience

I have been involved in the following courses in earlier years:

·  General Animal Biology

·  General and Comparative Animal Physiology

Current Master Projects

Anna Hronová

Investigation of changes in epigenetic modofications in rat brain induced by morphine treatment

Karol Červenák

The effect of cold acclimation on signaling mediated by G protein-coupled receptors in rat brain

Tereza Zelená

Study of fecal microbiome of rats exposed to chronic cold acclimation

Current PhD Projects

Bianka Mičke
Metadone and its effect on the fecal microbiome and metabolome of rats

Eliška Přítulová
Effect of methadone and its withdrawal on epigenetic regulations and transcription factors in rat brain

Daniela Nováková
The effect of methadone dependence and withdrawal on neurotransmitter systems: in vitro and in vivo studies 

Pinky Sultana
Deciphering the molecular mechanisms underpinning astrocyte senescence

Akash Mali
Opioid and metabolic modulation of microglial function

Ondřej Honc
A study of opioid signaling in astrocytes

Completed Bachelor, Master, and PhD Theses

GRANTS & CONTRACTS

I am/was involved in the following research projects:

• Circadian regulation of kynurenine pathway enzymes in rat brain and peripheral organs (Czech Science Foundation, grant no. 23-07184S, 2023-2025)

• Effects of acetylcholinesterase inhibition on cardiovascular system and cardiac ischemic tolerance in spontaneously hypertensive rats (Czech Science Foundation, grant no. 21-03810, 2021-2024)

• Consequences of sustained morphine treatment and withdrawal on the rat brain: proteomic and functional studies (Czech Science Foundation, grant no. 19-03295S, 2019-2021)

• Cardioprotective Potential of Cold Acclimation in Rats: The Role of Uncoupling Proteins (Czech Science Foundation, grant no. 17-07748S, 2017-2019)

• Maternal Separation in a Model of Early Life Stress-induced Psychosis (Czech Science Foundation, grant no. 16-13399S, 2016-2018)

• Interaction between Sustained Forms of Cardioprotection Induced by Adaptation to Chronic Hypoxia and Regular Exercise Training (Czech Science Foundation, grant no. P303/12/1162, 2012-2015)

• The Role of the STAT Signalling Pathway in the Rat Circadian System (Czech Science Foundation, grant no. P303/12/1137, 2012-2014)

• Molecular Mechanisms of Adverse and Protective Effects of Morphine and Methadone on the Rat Heart (Grant Agency of the Academy of Sciences of the Czech Republic, grant no. IAA501110901, 2009-2013)

• The Role of Adrenergic Signaling and Oxidative Stress in Molecular Mechanisms of Cardioprotection (Grant Agency of the Academy of Czech Republic, grant no. IAAX501110901, 2009-2013)

• Cellular and Molecular Physiology of Cardiomyocytes (Ministry of Education of the Czech Republic, grant no. MEB0810151, 2010-2011)

• Signaling and Molecular Mechanisms of Cell Response (Ministry of Education of the Czech Republic, grant no. MSM0021620858, 2007-2013)

• δ-Opioid Receptor Targeting to Membrane Domains: The Role of Palmitoylation (Grant Agency of the Academy of Sciences of the Czech Republic: grant no. A500110606, 2006-2008)

• Subcellular Distribution of μ- and δ-Opioid Receptors and Functional Activity of Trimeric G Proteins after Long-term Stimulation by Morphine (Czech Science Foundation, grant no. 309/06/0121, 2006-2008)

• The Role of Trimeric GTP-binding Proteins in the Mechanism of Hormone Action (Czech Science Foundation, grant no. 309/01/0255, 2001-2003)

• Center for Molecular and Cell Immunology (Ministry of Education of the Czech Republic, grant no. LN00A026, 2000-2004)

• Physiological Role of the Long and Short Form of Gsα Protein (Czech Science Foundation, grant no. 305/01/1660, 2000-2002)

• Regulation and Signaling in Living Systems (Ministry of Education of the Czech Republic, grant no. MSM113100003, 1999-2004)

• Center for Molecular Physiology and Immunology (Ministry of Education of the Czech Republic, grant no. VS97099, 1997-2000)