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Protocol
Notice: 0.5x TAE buffer should cover the agarose gel!
Prepare dots of 6x Loading Dye onto the surface of Parafilm.
Mix with 5 ul of DNA samples (except 10 ul for restriction enzyme-digested products)
Load resulting samples into the wells of the agarose gel
Load 5 ul of 1kb DNA marker / DNA ladder
Run in the electrophoresis tank for 30 min at 100 V
Observe the result with Omega Lum G Imaging System
Prepare 1% agarose gel:
Measure 100 ml of ddH2O in a 250-ml glass bottle.
Add 1 ml of 50x TAE Buffer into the ddH2O.
Weigh 1 g of Agarose Powder and mix with the buffer in the bottle.
Microwave 1 min → 30 sec → 30 sec → ...... until the agarose dissolves.
Cool at room temperature for 30 min
Pour into the gel tray with a comb.
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