DNA Polymerase Chain Reaction (PCR)

Materials

1. Template DNA: 10 ng/ul

2. Primer pairs: 10 μM for each

3. DNA polymerase

4. dNTPs (N = A, T, C, G): 10 mM

5. MgSO4: 25 mM

6. Buffer: 10x

Time needed: 2hr 10 min