DNA Polymerase Chain Reaction (PCR)
Materials
Template DNA: 10 ng/ul
Primer pairs: 10 μM for each
DNA polymerase
dNTPs (N = A, T, C, G): 10 mM
MgSO4: 25 mM
Buffer: 10x
Protocol
Time needed: 2hr 10 min
PCR - Polymerase Chain Reaction - Simple Animated Tutorial (Credit: @MrSimpleScience)
PCR - Polymerase Chain Reaction - Simple Animated Tutorial (Credit: @MrSimpleScience)
PCR 學習單
PCR 學習單
2X PCR Master Mix
2X PCR Master Mix
KOD plus DNA polymerase
KOD plus DNA polymerase
