Burn injuries are among the most physiologically and psychologically disruptive forms of trauma, and their sensory consequences are a major contributor to long-term morbidity. Burn patients often describe sensations that do not match what the skin looks like, including burning, prickling, hypersensitivity, numb patches, or relentless itch that is hard to relieve, despite their wounds appearing “healed”. One potential reason for this is that burns disrupt the skin’s smallest sensory nerve endings, the fine fibers that normally reach into the epidermis to detect temperature, irritation, and pain. When these fibers are damaged, they may not regrow properly, which can leave the healed skin sending mixed or exaggerated signals that contribute to chronic discomfort. This work focuses on building a reliable way to measure those changes. A standardized protocol was developed to punch biopsy skin, immunostain tissue to label nerve fibers and key molecular signals, and image the sections with confocal laser microscopy so nerve structure and neuropeptide expression can be quantified. The initial data presented come from a validation study confirming two core features of damaged skin, altered small-fiber innervation and shifted neuropeptide signaling. This study lays the groundwork that could eventually help flag patients at risk for chronic symptoms and point toward new targets for therapies aimed at post-burn pain and itch.