This study investigated whether FLS2 genes from different flowering plant species could be assembled into functional constructs for future testing in Nicotiana benthamiana mutants that lack their native FLS2 receptor. Using Golden Gate assembly, PCR amplification, and bacterial transformation, plasmids containing sgRNA1, sgRNA3, AmCyan, and FLS2 fragments within the pEE083 vector were created successfully. As seen, clear PCR bands, correct plasmid maps, robust colony growth, and infiltration confirmed that each step of the cloning process worked as intended.