AGAR

4% AGAR

  1. 10g of agar in 500ml bottles (8 bottles)

  2. bring up to 250ml of H2O

  3. autoclave

TE note: We use BD Difco Agar, bacteriological (CAT:214530). The technical grade of agar will impact the gel properties, color, and clarity (ie, number of non-dissolving particulates) that you see in your agar plates.



(And in the 'I can't believe I'm writing this department..') Agar is NOT the same as agarose. Agarose is commonly used to resolve different sized DNA fragments in conjuction with electrophoretic techniques. Agar is used to support the growth of bacterial cultures on solid media so that the microbes form colonies.