Ananda Maryam Jamila
Undergraduate Student of Faculty of Medicine, Jember University, Jember, Indonesia

Azham Purwandhono
Pathology Anatomy Department, Faculty of Medicine, Jember University, Jember, Indonesia

DOI: https://doi.org/10.19184/ams.v11i1.53712

Abstract

This study aimed to evaluate the acute oral toxicity of the flavonoid rutinoside on liver histopathology in Wistar rats, following OECD Guideline No. 423. Twelve Wistar rats were divided into four groups: male control and female control receiving oral administration of 5% dimethyl sulfoxide (DMSO), and treatment groups of male and female rats receiving rutinoside at a dose of 5000 mg/kg body weight (BW) in 5% DMSO orally. Post-treatment, liver histopathological examinations were performed to assess potential toxicological effects. The results indicated no signs of acute toxicity, as no significant histopathological alterations were observed in the liver tissues of the treated groups compared to the control groups. This study suggests that oral administration of rutinoside at 5000 mg/kg BW does not induce acute liver toxicity in Wistar rats under experimental settings.

Keyword: Acute oral toxicity, liver, rutinoside

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