Dwi Setyohadi
Department of Nutrition, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia

Wahyuni
Department of Biomedic, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia

Risma Yulfa Riyani
Undergraduate Student Program, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia

Khansa Khafiyya ‘Ibadurrahman
Undergraduate Student Program, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia

Andifa Anugerah Putra
Undergraduate Student Program, Faculty of Mathematic and Natural Science, Lambung Mangkurat University, Banjarmasin, Indonesia

DOI: https://doi.org/10.19184/ams.v10i1.43095

Abstract

Carica papaya is one of the plants known to increase appetite. 15% papaya leaf solution can increase appetite, but if given in 20% solution there is a weight loss effect. Papaya leaves contain papain, carpaine and various other compounds. The mechanisms that affect appetite regulation involve ghrelin, leptin, ARC, IL-1β and NPY. This study aims to determine the compound profile contained in papaya leaf extract and its effect on appetite regulation that has been analyzed with in silico methods. The method of determining the profiling of papaya leaf extract compounds using UPLC / MS and analyzed using watermaslinx, in silico analysis used molecular docking methods using PatchDock. The results of this study are the most compounds found in papaya leaf extract (Carica papaya) is carpaine with a concentration of 50.33%. The results of in silico analysis show that carpaine is predicted to have an effect on both reducing appetite and increasing appetite because carpaine has a stable bond with ghrelin but also has a small binding energy with leptin. In vivo research needs to be carried out to find out the right dose of carpaine to reduce or increase appetite.

Keyword: Carpaine; leptin, ghrelin, molecular docking, appetite.

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