Anak Agung Istri Ratnadewi 

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember  

Selvina Rizky Aprilia

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Bambang Piluharto

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Ana Yulvia

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

DOI: https://doi.org/10.19184/ICL.v2i2.5607

ABSTRACT 

Free endo-β-1,4-D-xylanase cannot be used more than once, so it needs to be modified by immobilizing the enzyme. Endo-β-1,4-D-xylanase was obtained from termite abdomen sources by isolation, ammonium sulfate purification, and dialysis methods. Endo-β-1,4-D-xylanase was immobilized with an alginate/nanocellulose matrix. This study aims to determine the activity, protein content, and repeated use of immobilized Endo-β-1,4-D-xylanase. This study used variations of Alginate/nanocellulose (0; 2.5; 5; 7.5; 10) %. Protein levels of Endo-β-1,4-D-xylanase were tested using the Bradford method and activity using the Miller method. The total protein bound to the immobilized Endo-β-1,4-D-xylanase was stated with the immobilized yield data. The immobilized yield with the composition of Alginate Nanocellulose (ANC) (0%) was 45.33% greater than the other compositions. Immobilized Endo-β-1,4-D-xylanase activity is efficient. ANC 5% produces an efficiency of 62.384% at the 12th hour, which is greater than the other ANC compositions.   

Keywords: endoxylanase, nanoparticle, xylooligosaccharide