Zulfikar

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember  

Antje Labes

Department of Energy and Biotechnology, Flensburg University and Applied Sciences, Germany

DOI: https://doi.org/10.19184/ICL.v1i2.206

ABSTRACT 

The present study has demonstrated the HPLC method for identification and determination  and -acids in Columbus hops. The measurement conditions has been set up included the use of reverse phase C18 column Chromolith, gradient elution with methanol (A) and Acetonitril acidified with phosphate buffer at pH 2.8. The flow rate has been applied of 0.8 mL/min, and run for 25 minutes. In addition, pretreatment sample has been done using SPE with the cartridge of C18 and the separation has been monitored using UV variable detector in the range of 300-340 nm. The results found that a four predominant peaks for α and β-acids, namely cohumulone (6.6 min), the second peak represents a mixture of humulone and adhumulone (8.9 min), the third peak is colupulone (15.9 min) and the fourth is a mixture of lupulone and adlupulone (16.7 min). The quantity of sample Columbus Hops has been determined according to multipoint calibration using ICE2 standard, and it is found that the sample contained 0.269 mg/mL of cohumulone, 0.341 mg/mL of humulone and adhumulone, 0.239 mg/mL of Colupulone and 0.141 mg/mL of mixture of lupulone and adlupulone. In addition, a high precision of measurement performed that is indicated by the value of RSD less than 5%.   

Keywords:  and b-acids of Columbus hops, SPE, Reverse Phase; HPLC.