When quantifying a target sequence using RT-PCR, it becomes difficult to detect errors in standards used as references, so the use of digital PCR (dPCR) provides a more accurate and absolute quantification of the sample. (Hindson et al., Salipante et al). The main difference between RT-PCR and dPCR is how the sample is prepared for amplification. In RT-PCR, a single sample is monitored after each cycle of amplification by an indicator consisting of a fluorescent probe. The probe will fluoresce based on the abundance of a specific sequence. In dPCR, the initial sample is divided into thousands of smaller samples of single template strands, then amplification is started. Endpoint PCR is performed where the fluorescence of the sample is monitored after the sample is finished amplifying. Ideally, no more than half of the wells should contain a template strand because the sample was split into thousands of parts. The wells which test positive for the amplification of the target DNA will fluoresce. DPCR utilizes Poisson statistics to help calculate ultra-precise results by correcting for rare instances where a well might have more than one strand present (Salipante et al.).