The findings strongly supported both hypotheses: extra-cusp-related traits (metaconule, hypoconulid) showed the greatest ancestral divergence (difference = 52%), while 20th-century samples demonstrated significant frequency homogenization (35% reduced intergroup variation).
This empirically validated Edgar's (2012) admixture models while addressing a literature gap - prior studies focused either on pre-1900 or modern samples, but none tracked continuous morphological change across this transitional period.
By analyzing 1,127 individuals spanning two centuries, this research established: 1) which traits remain stable ancestry markers despite admixture, and 2) quantitative benchmarks for temporal variation in dental trait frequencies, crucial for improving forensic identification accuracy in historically mixed populations.
Current approaches to forensic anthropology can benefit from this research as it strongly supports the use of extra-cusp related traits in the estimation of ancestry in historic American contexts, therefore suggesting they would have the highest degree of success in forensic or 21st century contexts.
It also suggests that the frequency rates for White and Black Americans have began to homogenize as the United States progressed into the 20th century, an interesting observation to note as forensic anthropologists may begin to use outdated data to estimate ancestry as time progresses.