ORGANIC cHEMISTRY - II

BSCM302

Experiment 5

Aim of the Experiment

To prepare Phenyl-glucosazone from Glucose.

Principle

Glucose forms osazone when it is treated with excess of phenyl hydrazine.

Materials required

Chemicals:

Glucose 2 g

Phenyl hydrazine (liquid) 4 ml

Glacial acetic acid 4 ml

Apparatus:

Beaker, water bath, filteration set, boling tube (if available)

Procedure

Dissolve glucose (2 g ) in about 10 ml of water in a boiling tube. Add 4 ml of phenyl hydrazine and 4 ml of glacial acetic acid to the above boling tube.Heat the mixture in a water bath for about an hour with constant shaking.The osazone separates as yellow solid.The tube is cooled,filtered at the pump and dried.Recrystallize from alcohol m.p. above 200 ⁰C

Exp-5 preparation of phenyle glucasone.mp4

Observation:

Amount of Glucose taken = g

Amount of glucosazone formed = g

Calculation:

180 g of glucose gives 358 g of glucosazone

Therefore,2 g of glucose would give __________?

(x) g of glucosazone X = 358 x 2 /180 = g

Experimental yield = g

^{%Yield =}x 100 %

Result & Conclusion

i) Yield of glucosazone = %

ii) Melting point of glucosazone = ⁰C. (Reported value =204°C)

Reference Material:

Glucosazone

Questions

What is difference between fructose and glucose.

Our table sugar known as a?

Developed by

Dr. Parin Kanaiya,

Assistant Professor, Chemistry

parin.kanaiya@gsfcuniversity.ac.in

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