有機分析
Organic Analysis
Organic Analysis
授課教師：林振東 / ctlin@thu.edu.tw
課程目標及內涵
無論是自然界取得的化合物或者人工合成的產物，為了鑑定分析構造式，必須使用各種分析、鑑定的技術，除化學反應方法外，微量的分析常使用圖譜判定。本課程的教學內容包括：
一、樣本的純化、分離、及化學鑑定介紹。
二、化合物之官能基與紅外線之吸收波長關係。
三、核磁共振光譜儀原理及化合物之結構鑑定。
四、可見光─紫外線光譜儀之運用。
五、質譜儀構造原理及分子碎片分析，由圖譜的綜合分析過程，訓練學生具有鑑定有機化合物結構的能力。
綜合紅外線光譜圖、核磁共振光譜圖、可見光─紫外線光譜圖、及質譜圖等判斷，解析有機化合物之分子結構。
主要內容：
一、化合物之官能機與紅外線之吸收波長關係。
1.分子伸張與彎曲振動
2.各種鍵結的振動吸收光譜波數
3.判圖練習
二、核磁共振光譜儀原理及化合物之結構鑑定。
1.核磁共振能量吸收機制
2.化學位移的定義
3.信號的積分值意義
4.自旋-自旋交互作用與能階分裂規則
5.氫核磁共振光譜判圖練習
6.碳核磁共振光譜
7.二維核磁共振光譜-同核二維相對光譜與異核二維相對光譜
三、可見光─紫外線光譜儀之運用。
1.可見光─紫外線吸收光譜原理
2.溶劑與分子共軛鑑結的影響
3.共軛雙烯、共軛烯酮的伍德沃德定則
四、質譜儀構造原理及分子碎片分析。
1.分子離子化方法
2.質量分析
3.決定分子式
授課教師：林振東 / ctlin@thu.edu.tw
Course Objectives and Contents:
Regardless of whether it is a compound obtained from nature or a synthetic product, in order to identify and analyze the structural formula, various analysis and identification techniques must be used. In addition to chemical reaction methods, trace analysis often uses spectrum determination. The teaching content of this course includes:
1. Introduction to purification, separation, and chemical identification of samples.
2. The relationship between the functional group of the compound and the absorption wavelength of infrared rays. 3. Principle of nuclear magnetic resonance spectrometer and structural identification of compounds.
4. Application of visible light-ultraviolet spectrometer.
5. The construction principle of mass spectrometer and the analysis of molecular fragments. Through the comprehensive analysis process of the spectrum, students are trained to have the ability to identify the structure of organic compounds. Analyze the molecular structure of organic compounds by comprehensively judging from infrared spectrum, nuclear magnetic resonance spectrum, visible light-ultraviolet spectrum, and mass spectrum.
Main content:
(1)、 The relationship between the functional mechanism of compounds and the absorption wavelength of infrared radiation.
1. Molecular stretching and bending vibration
2. Vibration absorption spectral wavenumber of various bonds
3. Drawing practice
(2). Principle of nuclear magnetic resonance spectrometer and structural identification of compounds.
1. Energy absorption mechanism of NMR
2. Definition of chemical shift 3. Significance of integral value of signal
4. Spin-spin interaction and energy level splitting rules
5. Exercise of hydrogen NMR spectrum judgment
6. Carbon NMR spectrum
7 .Two-dimensional nuclear magnetic resonance spectroscopy-homonuclear two-dimensional relative spectroscopy and heteronuclear two-dimensional relative spectroscopy
(3). Application of visible light-ultraviolet spectrometer
1. The principle of visible light-ultraviolet absorption spectroscopy
2. The influence of solvent and molecular conjugation
3. Woodward's rule of conjugated diene and conjugated ketene
(4). Construction principle of mass spectrometer and analysis of molecular fragments.
1. Molecular ionization method
2. Mass analysis
3. Determine molecular formula
主要參考書籍/資料 (Textbooks and References)
1. Introduction to Spectroscopy.; Donald L. Pavia; Gary M. Lampman; George S. Kriz, J. R. Vyvyan, 5th edition, 2015, Brooks/Cole.
2. Spectrometric Identification of Organic Compounds.; Robert M. Silverstein; Francis X. Webster.; seventh edition; 2005 by John Wiley & Sons, Inc.