Archival Journal

1. Y.-H. Liu, M.-T. Chung, H.-C. Lin, T.-A. Lee, Y.-J. Cheng, C.-C. Huang, H.-M. Wu*, and Y.-C. Tung*, “Shaping Early Neural Development by Elevated Tissue Oxygen Tension: Insights from Multiomic Analysis on Human Cerebral Organoids,” Science Advances, vol. 11, eado1164, 2025.

2. D.-M. Chang, H.-H. Hsu, P.-L. Ko, T.-H. Hsieh, H.-M. Wu*, and Y.-C. Tung*, “Rapid Time-Lapse 3D Oxygen Tension Measurement within Hydrogels Using Widefield Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM) and Image Segmentation,” Analyst, vol. 149, p. 1727-1737, 2024. (Featured as the back cover image). 

3. S. Kannan, P.-L. Ko, H.-M. Wu, and Y.-C. Tung*, “Characterization of Single-Spheroid Oxygen Consumption Using a Microfluidic Platform and Fluorescence Lifetime Imaging Microscopy,” Biosensors, vol. 14, P. 96, 2024. 

4. H.-H. Hsu, P.-L. Ko, C.-C Peng, Y.-J. Cheng, H.-M. Wu, and Y.-C. Tung*, “Studying Sprouting Angiogenesis Under Combination of Oxygen Gradients and Co-Culture of Fibroblasts Using Microfluidic Cell Culture Model,” Materials Today Bio, vol. 21, p. 100703, 2023.

5. S. Kannan, P.-L. Ko, H.-M. Wu, and Y.-C. Tung*, “Efficient Single-Cell Oxygen Consumption Rate Characterization Based on Frequency Domain Fluorescence Lifetime Imaging Microscopy Measurement and Microfluidic Platform,” Biomicrofluidics, vol. 17, P. 054105, 2023. 

6. H.-H. Hsu, P.-L. Ko, H.-M. Wu, H.-C. Lin, C.-K. Wang, and Y.-C. Tung*, “Study 3D Endothelial Cell Network Formation under Various Oxygen Microenvironment and Hydrogel Composition Combinations Using Upside-Down Microfluidic Devices,” Small, vol. 17, p. 2006091, 2021. (Featured as the inside back cover image). 

7. H.-M. Wu, T.-A. Lee, P.-L. Ko, W.-H. Liao, T.-H. Hsieh, and Y.-C. Tung*, “Widefield Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM) for Accurate Measurement of Oxygen Gradients within Microfluidic Devices,” Analyst, vol. 144, p. 3494-3504, 2019. (Featured as the inside back cover image). 

8. H.-C. Shih, T.-A. Lee, H.-M. Wu, P.-L. Ko, W.-H. Liao, and Y.-C. Tung*, “Microfluidic Collective Cell Migration Assay for Study of Endothelial Cell Proliferation and Migration under Combinations of Oxygen Gradients, Tensions, and Drug Treatments,” Scientific Reports, vol. 9, p. 8234, 2019. 

9. C.-K. Wang*, W.-H. Liao, H.-M. Wu, and Y.-C. Tung, “One-Step Approach to Fabricating Polydimethylsiloxane Microfluidic Channels of Different Geometric Sections by Sequential Wet Etching Processes,” Journal of Visualized Experiments, 139, e57868, 2018. 

10. S. Sarkar, C.-C. Peng, C.-W. Kuo, D.-Y. Chueh, H.-M. Wu, Y.-H. Liu, P. Chen, and Y.-C. Tung*, “Study of Oxygen Tension Variation within Live Tumor Spheroids Using Microfluidic Devices and Multi-Photon Laser Scanning Microscopy,” RSC Advances, vol. 8, p. 30320–30329, 2018.

11. H.-M. Wu, T.-A. Lee, P.-L. Ko, H.-J. Chiang, C.-C. Peng, and Y.-C. Tung*, “Review of Microfluidic Cell Culture Devices for the Control of Gaseous Microenvironments in vitro,” Journal of Micromechanics and Microengineering, vol. 28, p. 043001, 2018. 

12. C.-K. Wang*, W.-H. Liao, H.-M. Wu, Y.-H. Lo, T.-R. Lin, and Y.-C. Tung*, “Single Step Sequential Polydimethylsiloxane Wet Etching to Fabricate a Microfluidic Channel with Various Cross-Sectional Geometries,” Journal of Micromechanics and Microengineering, vol. 27, p. 115003, 2017.

13. H.-M. Wu, Y.-H. Lin, T.-C. Yen, and C.-L. Hsieh*, “Nanoscopic Substructures of Raft-Mimetic Liquid-Ordered Membrane Domains Revealed by High-Speed Single-Particle Tracking,” Scientific Reports, vol. 6, p. 20542, 2016.

Books/Book Chapters

1. Y.-C. Tung*, H.-M. Wu, and T.-A. Lee, “CHAPTER 11 Ionic Liquid-based Physical Sensors,” in Ionic Liquid Devices, Cambridge: The Royal Society Chemistry, pp. 296–320, 2017 (ISBN: 9781788011815, Citation: 15).

Conference Papers / Presentation

1. C.-H. Lin, J.-W. Yi, H.-M. Wu*, “Monitoring Photosynthesis Efficiency during Plant Growth Using the Frequency-Domain Fluorescence Lifetime Imaging (FD-FLI) System,” The 21st International Conference on Automation Technology, Taipei, Taiwan, November 22-24, 2024.

2. C.-H. Lin, J.-W. Yi, H.-M. Wu*, “Analyzing Photosynthetic Efficiency Based on Frequency-Domain Fluorescence Lifetime Imaging (FD-FLI) System,” 2024 Conference on Agricultural Machinery and Bio-Mechatonics Engineering, Taichung, Taiwan, October 24-25, 2024.

3. C.-H. Lin, J.-W. Yi, H.-M. Wu*, “Detection of Water Stress in Tomato Leaves Using Frequency-Domain Chlorophyll Fluorescence Lifetime Imaging System,” The 11th International Symposium on Machinery and Mechatronics for Agriculture and Biosystems Engineering (ISMAB 2024), Bali, Indonesia, September 27-29, 2024.

4. J.-W. Yi, C.-H. Lin, H.-M. Wu*, “Reconstruction of 3D Plant Model with the Fusion of RGB and Fluorescence Imaging System,” The 11th International Symposium on Machinery and Mechatronics for Agriculture and Biosystems Engineering (ISMAB 2024), Bali, Indonesia, September 27-29, 2024.

5. H.-M. Wu, W.-J. Chang, T.-A. Lee, and Y.-C. Tung*, “Real-Time Intracellular Oxygen Monitoring within Microfluidic Devices Using Widefield Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM),” 2023 IEEE 16th International Conference on Nano/Molecular Medicine &Engineering (NANOMED), Okinawa, Japan, December 5-8, 2023.

6. H.-M. Wu, and Y.-C. Tung*, “Monitoring Live Cell Oxygen Microenvironments Using Microfluidic Devices and Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM),” International Conference on Multi-scale Science and Engineering (ICMSE 2023), Okinawa, Japan, July 18-20, 2023.

7. K.-H. Chiang, W.-J. Chang, H.-M. Wu, and Y.-C. Tung, “Angiogenic Blood Vessels Reconfigure/Attenuate Hypoxic Tumor Microenvironments in A Quantitative In Vitro Model,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 43, no. Suppl_1, p. A644-A644, May 2023.

8. S. Kannan, P.-L. Ko, H.-M. Wu, and Y.-C. Tung, “Development of Microfluidic Devices for Single-Cell Oxygen Tension Analysis,” the 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS), Hangzhou, China, October 23-27, 2022.

9. S. Kannan, P.-L. Ko, H.-M. Wu, and Y.-C. Tung, "Efficient Single-Cell Oxygen Consumption Rate Characterization Based on Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM) Measurement and Microfluidic Platform,” International Conference on Smart Sensors (ICSS 2022), Taichung, Taiwan, October 22-23, 2022.

10. H.-M. Wu, W.-J. Chang, C.-C. Peng, and Y.-C. Tung, “Study Tumor Oxygen Microenvironments in vitro within Microfluidic Devices Using Frequency-Domain Fluorescence Lifetime Imaging Microscopy,” Annual Meeting of Taiwanese Society of Biomedical Engineering (TSBME), Taichung, Taiwan, November 19-20, 2021.

11. H.-M. Wu, W.-J. Chang, T.-A. Lee, W.-H. Liao, and Y.-C. Tung, “Intracellular Oxygen Response Monitoring within Microfluidic Devices Using Widefield Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM),” the 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS), Palm Springs, USA, October 10-14, 2021 (Distinguished Oral Paper Award).

12. H.-M. Wu, W.-J. Chang, T.-A. Lee, W.-H. Liao, and Y.-C. Tung, “Intracellular Oxygen Response Monitoring within Microfluidic Devices Using Widefield Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM),” International Conference on Smart Sensors (ICSS 2021), Taipei, Taiwan, October 14-15, 2021.

13. P.-L. Ko, H.-H. Hsu, H.-M. Wu, and Y.-C. Tung, " Toward a Systemic in vitro Model: Microfluidic Cell Culture,“ Annual Meeting of Taiwan Nanomedicine Society, Taipei, Taiwan, November 28, 2020.

14. H.-M. Wu, W.-J. Chang, T.-A. Lee, X. D. Wang, and Y.-C. Tung, “Intracellular Oxygen Measurement with Subcellular Resolution Using Widefield Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM),” Focus on Microscopy 2020 (FOM 2020) Conference, Osaka, Japan, April 5-8, 2020. (Canceled due to COVID-19)

15. D.-M. Chang, H.-H. Hsu, P.-L. Ko, H.-M. Wu, and Y.-C. Tung, “Characterization of 3D Oxygen Tension Profiles within Hydrogels Using Oxygen Sensitive Fluorescence Microbeads and Widefield Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM),” Focus on Microscopy 2020 (FOM 2020) Conference, Osaka, Japan, April 5-8, 2020. (Canceled due to COVID-19)

16. Y.-H. Liu, H.-M. Wu, C.-C. Huang, and Y.-C. Tung, “Long-Term Monitoring of Oxygen Tension in Living Cerebral Organoids Using Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM),” Focus on Microscopy 2020 (FOM 2020) Conference, Osaka, Japan, April 5-8, 2020. (Canceled due to COVID-19)

17. H.-H. Hsu, P.-L. Ko, H.-M. Wu, T.-A. Lee, H.-C. Lin, and Y.-C. Tung, “Development of a Microfluidic Device Capable of Generating Oxygen Gradients for Three-Dimensional Cell Culture in Hydrogel,” the 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS), Basel, Switzerland, October 27-31, 2019.

18. H.-M. Wu, T.-A. Lee, P.-L. Ko, W.-H. Liao, and Y.-C. Tung, “Accurate Oxygen Tension Profile Measurement within Microfluidic Devices Using Frequency Domain Fluorescence Lifetime Microscopy (FD-FLIM),” the 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS), Kaohsiung, Taiwan, November 11-15, 2018.

19. H.-C. Shih, T.-A. Lee, H.-M. Wu, P.-L. Ko, and Y.-C. Tung, “Study Effects of Hypoxia-Inducible Factor (HIF) Inhibitor on Endothelial Cell Migration under Oxygen Gradients Using Microfluidic Wound Healing Assay,” the 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS), Kaohsiung, Taiwan, November 11-15, 2018.

20. S. Sarkar, D.-Y. Chueh, C.-C. Peng, H.-M. Wu, P. Chen, and Y.-C. Tung, “Study of Hypoxic Cores within Live Tumor Spheroids Using Microfluidic Devices and Multi-Photon Microscopy,” EMBL Conference: Microfluidics 2018: New Technologies and Applications in Biology, Biochemistry and Single-Cell Analysis, Heidelberg, Germany, July 15-17, 2018.

21. Y.-C. Tung, H.-C. Shih, H.-M. Wu, and W.-H. Liao, “Study Endothelial Cell Migration under Oxygen Gradients Using Microfluidic Devices,” International Joint Symposium of CEFMS-NCTU, RCAS-AS (Taiwan) and 5-Star Alliance (Japan), Hsinchu, Taiwan, May 18-20, 2018. (Invited Talk)

22. H.-M. Wu, Y.-H. Lin, T.-C. Yan, and C.-L. Hsieh, “Nano-Substructures of Raft-Mimetic Liquid-Ordered Membrane Domains Revealed by High-Speed Single-Particle Tracking,” Biophysical Journal, vol. 110, no. 3, p. 568a, 2016.

23. C.-Y. Chou, H.-M. Wu, Y.-H. Lin, and T.-C. Yan, and C.-L. Hsieh, “Investigation of Membrane Domains and Single-Molecule Dynamics in Lipid Bilayers Using Interferometric Scattering (iSCAT) Optical Microscopy,” Annual Meeting of the Physical Society of the Republic of China, Taipei, Taiwan, Jan. 25-27, 2016.

24. 羅光閔,郭家閎,孫士璋,吳筱梅,“FRP-金屬異質接合設計與機械強度測試,”海軍軍官學校海洋科學與應用學術研討會, Sep. 13. 2016.

25. 羅光閔,李雅榮,賴建璋,孫士璋,吳筱梅,“BV規範應用於軍艦FRP上層結構設計,”海軍軍官學校海洋科學與應用學術研討會, Sep. 13. 2016.

26. H.-M. Wu, Y.-H. Lin, T.-C. Yan, and C.-L. Hsieh, “Direct Visualization of Nanoscopic Diffusion Path of Single Lipid Molecules in Raft-Containing Bilayer Membranes with Microsecond Temporal Resolution,” New Biological Frontiers Illuminated by Molecular Sensors and Actuators held by Biophysical Society, Taipei, Taiwan, Jun. 28-Jul. 1, 2015.

27. H.-M. Wu, Y.-H. Lin, and C.-L. Hsieh, “High-Speed Direct Visualization of Nanoscopic Diffusion Path of Single Lipid Molecules in Raft-Containing Bilayer Membranes,” Single-molecule Biophysical Chemistry Workshop, Taipei, Taiwan, Apr. 23, 2015.

28. Y.-H. Lin, H.-M. Wu, and C.-L. Hsieh, “High-Speed Single-Particle Tracking: Application to Molecular Diffusion in Biological Membranes,” Optics in the Life Science, no. 1, p. NM3C.4, 2015.

29. Y.-H. Lin, H.-M. Wu, and C.-L. Hsieh, “High-Speed Single-Particle Tracking Reveals Lipid Dynamics in Heterogeneous Raft-Containing Membranes,” Biophysical Journal, vol. 108, no. 2, p 79a, 2015.

30. H.-M. Wu, A. Tokranov, X. Xiao, Y. Qi, M. W. Verbrugge, B. W. Sheldon, and K.-S. Kim, “Characterization of Interfacial Sliding Properties at Amorphous Si/Cu Interface due to Li-ion Intercalation by Using Self-Adjusting Liquid Linnik Interferometer,” 17th U.S. National Congress on Theoretical and Applied Mechanics, East Lansing, MI, Jun. 15-20, 2014.

31. H.-M. Wu, J. W. Yi, M.-W. Moon, and K.-S. Kim, “Nanobearings in Nature,” Future Directions in Mechanics Research, NSF Workshop and Symposium in honor of Professor L. B. Freund, Providence, RI, Jun. 1-3, 2011.

32. C.-K. Wang, L.-J. Leu, and H.-M. Wu, “Application of FE package ABAQUS for Evolutionary Structural Optimization,” The 20th KKCNN Symposium on Civil Engineering, Jeju, South Korea, Oct. 4-6, 2007.