32. Atsushi Mukaiyama*#, Yoshihiko Furuike*#, Kumiko Ito-Miwa, Yasuhiro Onoue, Kota Horiuchi, Kanta Kondo, Eiki Yamashita, and Shuji Akiyama*, “Evolutionary origins of self-sustained Kai protein circadian oscillators in cyanobacteria” Nat Commun 16, 4541 (2025). ＊責任著者、#共同筆頭著者
日本語プレスリリース（最古の体内時計が日の出を知らせた約22億年前のある日） Press release in English
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31. Tomonao Inobe*, Runa Sakaguchi, Takayuki Obita, Atsushi Mukaiyama, Siichi Koike, Takeshi Yokoyama, Mineyuki Mizuguchi and Shuji Akiyama, “Structural insights into rapamycin-induced oligomerization of a FRB-FKBP fusion protein” FEBS Letters
30. Akari Nishi, Azumi Hirata, Atsushi Mukaiyama, Shun-ichi Tanaka, Ryota Nomura, Kazuhiko Nakano and Kazufumi Takano*, “Role of N1-Domain, Linker, N2-Domain, and Latch in the Binding Activity and Stability of the Collagen-Binding Domain for the Collagen-Binding Protein Cbm from Streptococcus mutans” Physchem 4, 120-130 (2024)
29. Akari Nishi, Hikaru Matsui, Azumi Hirata, Atsushi Mukaiyama, Shun-ichi Tanaka, Takuya Yoshizawa, Hiroyoshi Matsumura, Ryota Nomura, Kazuhiko Nakano and Kazufumi Takano*, “Structure, Stability and Binding Properties of Collagen-Binding Domains from Streptococcus mutans” Chemistry 5 1911-1920 (2023)
28. Mukaiyama A*#, Furuike Y#, Yamashita E and Akiyama S, “Highly Sensitive Tryptophan Fluorescence Probe for detecting Rhythmic Conformational changes of KaiC in the Cyanobacterial Circadian Clock system”, Biochemical Journal 479, 1505-1515 (2022) ＊責任著者、#共同筆頭著者
27. Furuike Y, Mukaiyama A, Koda S, Simon D, Ouyang D, Ito-Miwa K, Saito S, Yamashita E, Nishiwaki-Ohkawa T, Terauchi K, Kondo T and Akiyama S, “Regulation mechanisms of the dual ATPase in KaiC”, Proc. Natl. Acad. Sci. USA. 119, e2119627119 (2022).
26. Furuike Y#, Mukaiyama A#, Ouyang D, Ito-Miwa K, Simon D, Yamashita E, Kondo T and Akiyama S,“Elucidation of master allostery essential for circadian clock oscillation in cyanobacteria”, Sci. Adv. 8, eabm8990 (2022). #共同筆頭著者
25. Furuike Y, Ouyang D, Tominaga T, Matsuo T, Mukaiyama A, Kawakita Y, Fujiwara S and Akiyama S,“Cross-scale analysis of temperature compensation in the cyanobacterial circadian clock system”, Commun. Phys. 5, 75 (2022).
24. Simon D, Mukaiyama A, Furuike Y and Akiyama S, “Slow and temperature-compensated autonomous disassembly of KaiB-KaiC complex”, Biophys. and Physicobiol. 19, e190008 (2022).
23. Maruyama M, Furukawa Y, Kinoshita M, Mukaiyama A, Akiyama S and Yoshimura T, Adenylate kinase 1 overexpression increases locomotor activity in medaka fish”, PLoS One. 17, e0257967 (2022).
22. Mukaiyama A, Ouyang D, Furuike Y and Akiyama S,“KaiC from a cyanobacterium Gloeocapsa sp. PCC 7428 retains functional and structural properites required as the core of the clock system”, Int. J. Biol. Macromol. 131, 67-73 (2019).
21. Ouyang D, Furuike Y, Mukaiyama A, Ito-Miwa K, Kondo T and Akiyama S, “Development and optimization of expression, purification and ATPase assay of KaiC for medium throughput screening of circadian clock mutants in cyanobacteria”, Int. J. Mol. Sci. 20, 2789-2800 (2019).
20. Mukaiyama A*, Furuike Y, Abe J , Koda S, Yamashita E, Kondo T and Akiyama S*,“Conformational rearrangements of the C1 ring in KaiC measure the timing of assembly with KaiB”, Sci. Rep. 8:8803 (2018). ＊共同責任著者
19. Anzai I, Tokuda E, Mukaiyama A, Akiyama S, Endo F, Yamanaka K, Misawa H and Furukawa Y, “A misfolded dimer of Cu/Zn-superoxide dismutase leading to pathological oligomerization in amyotrophic lateral sclerosis”, Protein Sci. 26, 484-496 (2017).
18. Furuike Y, Abe J, Mukaiyama A and Akiyama S, “Accelerating in vitro Studies on Circadian Clock Systems Using an Automated Sampling”, Biophys. and Physicobiol. 13, 235-241 (2016).
17. Anzai I, Toichi K, Tokuda E, Mukaiyama A, Akiyama S and Furukawa Y, “Screenings of Drugs Inhibiting In vitro Oligomerization of Cu/Zn-Superoxide Dismutase with a Mutation Causing Amyotrophic Lateral Sclerosis”, Front. Mol. Biosci. 3:40 (2016).
16. Furukawa Y, Suzuki Y, Fukuoka M, Nagasawa K, Nakagome K, Shimizu H, Mukaiyama A and Akiyama S, “A molecular mechanism realizing sequence-specific recognition of nucleic acids by TDP-43”, Sci. Rep. 6:20576 (2016).
15. Abe J#, Hiyama TB#, Mukaiyama A#, Son S#, Mori T#, Saito S, Osako M, Wolanin J, Yamashita E, Kondo T and Akiyama S, “Atomic-scale Origins of Slowness in the Cyanobacterial Circadian Clock”, Science 349, 312-316 (2015). #共同筆頭著者
14. Mukaiyama A, Osako M, Hikima T, Kondo T and Akiyama S,“A protocol for preparing nucleotide-free KaiC monomer”, BIOPHYSICS 11, 79-84 (2015).
13. Mahesh SC, Nakamura T, Makabe K, Takenaka T, Mukaiyama A, Tapan KC, Kato K, Kuwajima K, “The H/D-Exchange kinetics of the Escherichia coli Co-chaperonin GroES studied by 2D NMR and DMSO-Quenched Exchange Methods”, J. Mol. Biol. 425, 2541-2560 (2013).
12. Mukaiyama A#, Nakamura T#, Makabe K, Maki K, Goto Y and Kuwajima K, “The molten globule of β2-microglobulin accumulated at pH 4 and its role in protein folding”, J. Mol. Biol. 425, 273-291 (2013). #共同筆頭著者
11. Mukaiyama A#, Nakamura T#, Makabe K, Maki K, Goto Y and Kuwajima K, “Native-state heterogeneity of β2-microglobulin as revealed by kinetic folding and real-time NMR experiments”, J. Mol. Biol. 425, 257-272 (2013). #共同筆頭著者
10. Murayama Y#, Mukaiyama A#, Imai K#, Onoue Y, Tsunoda A, Nohara A, Ishida T, Maéda Y, Terauchi K, Kondo T and Akiyama S, “Tracking and visualizing the circadian ticking of the cyanobacterial clock protein”, EMBO J. 30, 68-78 (2011) #共同筆頭著者
09. Okada J, Okamoto T, Mukaiyama A, Tadokoro T, You D-J, Chon H, Koga Y, Takano K and Kanaya S,“Evolution and thermodynamics of the slow unfolding of hyperstable monomeric proteins”, BMC Evol. Biol. 10, 207 (2010).
08. Nakamura T, Makabe K, Tomoyori K, Maki K, Mukaiyama A and Kuwajima K,“Different folding pathways taken by highly homologous proteins, goat-a lactalbumin and canine milk lysozyme”, J. Mol. Biol. 396, 1361-1378 (2010).
07. Takano K, Higashi R, Okada J, Mukaiyama A, Tadokoro T, Koga Y and Kanaya S,“Proline effect on the thermostability and slow unfolding of a hyperthermophilic protein”, J. Biochem. 145, 79-85 (2009).
06. Dong H, Mukaiyama A, Tadokoro T, Koga Y, Takano K and Kanaya S,“Hydrophobic effect on the stability and folding of a hyperthermophilic protein” J. Mol. Biol. 378, 264-272 (2008).
05. Mukaiyama A, Koga Y, Takano K and Kanaya S, “Osmolyte Effect on the Stability and Folding of a Hyperthermophilic Protein”, Proteins 71, 110-118 (2008).
04. Takano K, Katagiri Y, Mukaiyama A, Chon H, Matsumura H, Koga Y and Kanaya S,“Conformational Contagion in a Protein: Structural Properties of a Chameleon Sequence”, Proteins 68, 617-625 (2007).
03. Mukaiyama A, Haruki M, Ota M, Koga Y, Takano K, and Kanaya S,“A hyperthermophilic protein acquires function at the cost of stability”, Biochemistry 45, 12673-12679 (2006).
02. Takano K, Endo S, Mukaiyama A, Chon H, Matsumura H, Koga Y, and Kanaya S,“Structure of amyloid b fragments in aqueous environments”, FEBS J. 273, 150-158 (2006).
01. Mukaiyama A, Takano K, Haruki M, Morikawa M, and Kanaya S,“Kinetically robust monomeric protein from a hyperthermophile”, Biochemistry 43, 13859-13866 (2004).
12. Furuike Y, Mukaiyama A and Akiyama S, "Mater allostery in clock protein KaiC orchestrates circadian rhythm", SPring-8 Research Frontiers 2022, 26-27 (2023).
11. [Proceedings] Mukaiyama A, Furuike Y and Akiyama S,“A kinetic mechanism gating the assembly of KaiB-KaiC in the cyanobacterial circadian clock system”, Biological Rhythms, 29-34 (2019).
10. 秋山 修志, 古池 美彦, 向山 厚, “変化し続ける概日時計研究のかたち”, 時間生物学 24, 92-99 (2018).
09. [Proceedings] Akiyama S, Mukaiyama A, Abe J and Furuike Y, “Cyanobacterial circadian clock system: how and why can it be so slow and stable?”, Biological Clocks: with reference to suprachiasmatic nucleus, 73-77 (2017).
08. Abe J, Mukaiyama A and Akiyama S, “Absolute Slowness encoded in the circadian clock protein” , SPring-8 Research Frontiers 2015, 24-25 (2016).
07. 向山 厚, 阿部 淳, 孫 世泳, 秋山 修志,“タンパク質の化学反応が細胞内の時を計る”, 実験医学 33, 3119-3122 (2015).
06. 向山 厚, “タンパク質が刻む概日リズム”, 生物工学会誌 96巻2号, 107 (2013).
05. Mukaiyama A, Kondo T, Akiyama S, “Visualization of circadian ticking of cyanobacterial clock protein KaiC in real time”, Spring-8 Research Frontiers 2011, 46-47 (2012).
04. 秋山 修志, 向山 厚, “時計タンパク質KaiCの概日性分子鼓動”, 実験医学 29, 1281-1284 (2011).
03. Mukaiyama A and Takano K,“Delineation of the Conformational Thermostability of Hyperthermophilic Proteins Based on Structural and Biophysical Analyses”, Thermostable Proteins: Structural Stability and Design (S. Sen & L. Nilsson, eds.) pp. 1-20, CRC Press (2011).
02. Mukaiyama A and Takano K,“Folding and Unfolding of Hyperthermophilic Proteins; Molecular Basis of Adaptation to Hot Environment”, Protein Folding (E. C. Walters, ed.) pp. 443-464, Nova Science (2011).
01. 向山厚, 高野和文,“超好熱菌由来蛋白質の熱力学的安定化機構”, 熱測定 35, 237-243 (2008).