High Capacity Protein A Membrane Adsorbers
Your Name: Joshua Osuofa
Authors: Scott Husson
Degree: Doctoral Candidate
Faculty Advisor/Mentor: Scott Husson
College: CECAS
Department: Chemical and Biomolecular Engineering
Email Address: josuofa@clemson.edu
Abstract
Chromatography is ubiquitous to therapeutic protein purification. When the protein is a monoclonal antibody (mAb), Protein A chromatography is used in the capture step to separate the product selectively from process impurities such as host cell proteins (HCPs), host cell DNA, viruses, charged variants, fragments, endotoxins and aggregates. Among the issues in resin-based Protein A chromatography is the low throughput associated with flowrate-dependent performance. In particular, diffusion limitations require resin columns to be operated with long residence times to achieve satisfactory binding capacities.
Protein A membrane chromatography is a solution. Recent innovations in Protein A membrane chromatography have led to products with binding capacities on par with resin chromatography using residence times that are shorter by an order of magnitude or more. The objectives of this study were to provide an introduction to a Protein A membrane developed in our lab as well as provide the first preliminary comparison of all commercial Protein A membranes in terms of dynamic binding capacity, regeneration-reuse, and pressure-flow rate relationships. This presentation will report findings from a comparison of Purilogics Purexa-A, Gore Protein Capture Device, Cytiva HiTrap Fibro Prism A, and Sartorius Sartobind® A chromatography columns.