The Effect of AgNP on Insect Feeding

Abstract

Silver Nanoparticles (AgNP) are nano-sized particles of silver that are used in various industries as an anti-microbial agent in fabrics and medical equipment. Recently, there has been a lot of concern about the lasting effects of these particles if they seep into ground water or enter the food supply. Previously, our group treated Arabidopsis plants with various concentrations of AgNPs, control solutions, or water over a 5-week period. Plants were then exposed to insect feeding by the generalist caterpillar, Spodoptera exigua for 48 hours. Insects were also weighed before and after feeding. Photographs of the plants were taken before and after the herbivory assay. My project entails analyzing insect health data and applying digital image analysis using a customized MATLAB algorithm that detects changes in plant leaf surface area in a non-destructive manner, including leaf tissue removed by insects. Because Arabidopsis plants have a relatively flat growth pattern (called a 'rosette'), they are a model system for 2D-image analysis. We have already shown that plant coloration as well as root and shoot growth are affected by the presence of AgNP treatment. The goal of my project is to find out whether AgNP treated plants deter or are more susceptible to insect feeding or if they disrupt insect health.

Cole Brunker | Biology and Chemistry | Faculty Sponsor Erin Rehrig