How to cite:
Ancheta, A. R. P., Acoba, P. P. C., Lopez, S. M. P., & Maines, M. S. F. (2026). Effects of a biodegradable seedling tray made from chicken manure and banana pseudostem fiber on the early growth performance of tomato (Solanum lycopersicum). Research Journal of Science Innovation, Anthropology, Sustainable Agriculture, and Technology, 1(1), 1–11. https://doi.org/10.5281/zenodo.19694006
In-Text Citations
Parenthetical:
(Ancheta et al., 2026)
Narrative:
Ancheta et al. (2026)
Angelee Rose P. Ancheta, Precious Paula C. Acoba, Shiela Mae P. Lopez, Midge Seiman F. Maines
https://doi.org/10.5281/zenodo.19694006
Abstract
The widespread reliance on conventional plastic seedling trays in horticulture exacerbates agricultural plastic pollution and soil degradation, necessitating the development of sustainable alternatives. This study evaluated the efficacy of biodegradable seedling trays, formulated from varying proportions of chicken manure, banana pseudostem fiber, and sawdust biochar, on the early vegetative growth of tomato (Solanum lycopersicum). Utilizing a completely randomized design, the research compared three experimental formulations against a commercial plastic control. Performance was assessed 14 days post-germination by measuring plant height and leaf count. Data were analyzed using a one-way analysis of variance and a Kruskal-Wallis test at a 0.05 significance level. Results demonstrated that the commercial control achieved the highest overall mean plant height and leaf count. However, among the biodegradable options, the formulation with the highest banana fiber content yielded significantly greater plant height (3.58 cm) compared to the reduced-fiber alternatives (F = 18.15, p < .001) and produced a significantly higher number of leaves (H = 13.81, p < .01). While structural integrity and biodegradation rates did not differ significantly across organic formulations, fiber content critically drove moisture dynamics. Although commercial trays facilitated more rapid initial growth, the high-fiber biodegradable trays supported continuous development, presenting a viable ecological alternative. Future studies should prioritize optimizing substrate formulations to enhance nutrient mineralization rates and immediate availability.
Keywords: biodegradable seedling tray, CHIMNARCH, tomato growth, sustainable agriculture, organic materials
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References
Amanullah, M. M., Sekar, S., & Vincent, S. (2010). Plant growth substances in crop production: A review. Asian Journal of Plant Sciences, 9(4), 215–222. https://doi.org/10.3923/ajps.2010.215.222
Anirudh, M., Lal, A. N., Harikrishnan, M., Jose, J., Thasim, J., Warrier, A. S., Venkatesh, R., Vaddevolu, U. B. P., & Kothakota, A. (2024). Sustainable seedling pots: Development and characterisation of banana waste and natural fibre-reinforced composites for horticultural applications. International Journal of Biological Macromolecules, 270(1), 132070. https://doi.org/10.1016/j.ijbiomac.2024.132070
Aryal, P., Vista, S. P., Dhakal, R., Basnet, B., Chand, P., Gyawali, S., & Pandit, N. R. (2024). Assessment of biochar quality and agronomic efficiency produced from rice-husk and saw-dust at different temperature regimes. Archives of Agriculture and Environmental Science, 9(4), 667–675. https://doi.org/10.26832/24566632.2024.090405
Aventurado, J. A. B., Belan, X. A. A., De Castro, J. N. T., & Manuel, D. V. (n.d.). Biodegradable plant pots made from Malus domestica (apple) pomace, Musa acuminata (banana) peel, and Cocos nucifera (coconut) fiber. Animo Repository. https://animorepository.dlsu.edu.ph/conf_shsrescon/2022/paper_men/2/
Badanayak, P., Jose, S., & Bose, G. (2023). Banana pseudostem fiber: A critical review on fiber extraction, characterization, and surface modification. Journal of Natural Fibers, 20(1). https://doi.org/10.1080/15440478.2023.2168821
Brady, N. C., & Weil, R. R. (2020). Solutions manual for nature and properties of soils (14th ed.). Testbank4Textbook. https://testbank4textbook.com/pdf_samples/Solutions_Manual_for_Nature_and_Properties_of_Soils_14th_Edition_by_Brady_sample_chapter.pdf
Briassoulis, D. (2023). Agricultural plastics as a potential threat to food security, health, and environment through soil pollution by microplastics: Problem definition. The Science of the Total Environment, 892, 164533. https://doi.org/10.1016/j.scitotenv.2023.164533
Chauhan, S., Gupta, T. K., & Srivastava, V. S. (2021). Applicability of banana fiber as reinforcement in composites. In Smart innovation, systems and technologies (pp. 77–91). https://doi.org/10.1007/978-981-16-2857-3_10
Chung, W. J., Chang, S. W., Chaudhary, D. K., Shin, J., Kim, H., Karmegam, N., Govarthanan, M., Chandrasekaran, M., & Ravindran, B. (2021). Effect of biochar amendment on compost quality, gaseous emissions and pathogen reduction during in-vessel composting of chicken manure. Chemosphere, 283, 131129. https://doi.org/10.1016/j.chemosphere.2021.131129
Coppola, G., Gaudio, M. T., Lopresto, C. G., Calabro, V., Curcio, S., & Chakraborty, S. (2021). Bioplastic from renewable biomass: A facile solution for a greener environment. Earth Systems and Environment, 5(2), 231–251. https://doi.org/10.1007/s41748-021-00208-7
Davis, A. J., & Strik, B. C. (2022). Long-term effects of pre-plant incorporation with sawdust, sawdust mulch, and nitrogen fertilizer rate on ‘Elliott’ highbush blueberry. HortScience, 57(3), 414–421. https://doi.org/10.21273/hortsci16359-21
Dev, C. V., A, A. A., Krishnan, A., & S, G. (2024). Stabilization of soil using sawdust ash, an ecofriendly approach. Zenodo. https://doi.org/10.5281/zenodo.18139203
Duarte, E. B., Bassani, F. N., Guzzoni, L. M., De Oliveira, D. M., Cardoso, F. A. R., & Herek, L. C. S. (2025). Biodegradabilidade de materiais reforçados com fibra de bananeira (Musa sp.) em matriz polimérica. Research Society and Development, 14(12), e67141250321. https://doi.org/10.33448/rsd-v14i12.50321
Elashry, M. E., Khater, E. G., & Ali, S. A. (2025). Biodegradable biocomposite pots reinforced with mercerized sugarcane bagasse for sustainable agriculture and plastic waste mitigation. Scientific Reports, 15(1), 17199. https://doi.org/10.1038/s41598-025-01419-y
Folino, A., Karageorgiou, A., Calabrò, P. S., & Komilis, D. (2020). Biodegradation of wasted bioplastics in natural and industrial environments: A review. Sustainability, 12(15), 6030. https://doi.org/10.3390/su12156030
Hsu, C., & Lai, H. (2022). Comprehensive assessment of the influence of applying two kinds of chicken-manure-processed organic fertilizers on soil properties, mineralization of nitrogen, and yields of three crops. Agronomy, 12(10), 2355. https://doi.org/10.3390/agronomy12102355
Ishara, G., Koliyabandara, P. A., & Samarakoon, G. (2024). Eco-friendly bio-composite sheets: A study on the utilization of banana peels, cassava starch, and banana stem fibers. Frontiers in Sustainability, 5, 1410986. https://doi.org/10.3389/frsus.2024.1410986
Jaya, J. D., Elma, M., Sunardi, S., & Nugroho, A. (2022). Physical and mechanical properties of biodegradable pot derived from oil palm empty fruit bunch and sodium alginate. Brazilian Archives of Biology and Technology, 65. https://doi.org/10.1590/1678-4324-2022210789
Juanga-Labayen, J. P., & Yuan, Q. (2021). Making biodegradable seedling pots from textile and paper waste—Part B: Development and evaluation of seedling pots. International Journal of Environmental Research and Public Health, 18(14), 7609. https://doi.org/10.3390/ijerph18147609
Kar, S., Reddy, M. K., Asthana, R., Srivastava, P., Dhaarani, R., Reddy, K. V. N. S., Meghamala, V., Koduru, J. R., & Karri, R. R. (2025). Synergistic effects of Syzygium cumini sawdust biochar and poultry manure on soil quality enhancement, nitrogen, organic carbon dynamics, and Amaranthus cruentus growth. Scientific Reports, 15(1), 43509. https://doi.org/10.1038/s41598-025-25003-6
Khodadadi, M., Masoumi, A., & Sadeghi, M. (2024). Drying, a practical technology for reduction of poultry litter (environmental) pollution: Methods and their effects on important parameters. Poultry Science, 103(12), 104277. https://doi.org/10.1016/j.psj.2024.104277
Ko, D., Chung, H., Park, J., Kim, H., Kang, E., Lee, S., & Yoon, T. K. (2023). Recycled waste leaf litter pots exhibit excellent biodegradability: An experimental analysis. Horticulturae, 9(9), 987. https://doi.org/10.3390/horticulturae9090987
Köninger, J., Lugato, E., Panagos, P., Kochupillai, M., Orgiazzi, A., & Briones, M. J. (2021). Manure management and soil biodiversity: Towards more sustainable food systems in the EU. Agricultural Systems, 194, 103251. https://doi.org/10.1016/j.agsy.2021.103251
Li, R., Cheng, X., Dai, P., Zhang, M., Li, M., Chen, J., Hassan, W. U., & Wang, Y. (2025). Plant architectural structure and leaf trait responses to environmental change: A meta-analysis. Plants, 14(11), 1717. https://doi.org/10.3390/plants14111717
Liu, L., Li, J., Wu, G., Shen, H., Fu, G., & Wang, Y. (2021). Combined effects of biochar and chicken manure on maize (Zea mays L.) growth, lead uptake and soil enzyme activities under lead stress. PeerJ, 9, e11754. https://doi.org/10.7717/peerj.11754
Ning, L., Xu, X., Zhang, Y., Zhao, S., Qiu, S., Ding, W., Zou, G., & He, P. (2022). Effects of chicken manure substitution for mineral nitrogen fertilizer on crop yield and soil fertility in a reduced nitrogen input regime of North-Central China. Frontiers in Plant Science, 13, 1050179. https://doi.org/10.3389/fpls.2022.1050179
Nguyen, T. A., & Nguyen, T. H. (2021). Banana fiber-reinforced epoxy composites: Mechanical properties and fire retardancy. International Journal of Chemical Engineering, 2021, 1–9. https://doi.org/10.1155/2021/1973644
Nzoyisaba, K., Mtaita, T. A., & Chakeredza, S. (2023). The influence of bio-slurry, chicken manure tea and vermi compost tea on growth and yield of tomatoes. International Journal of Plant Pathology and Microbiology, 3(2), 91–99. https://www.plantpathologyjournal.com/article/64/3-2-21-771.pdf
Pahalvi, H. N., Rafiya, L., Rashid, S., Nisar, B., & Kamili, A. N. (2021). Chemical fertilizers and their impact on soil health. In Microbiota and biofertilizers (Vol. 2, pp. 1–20). https://doi.org/10.1007/978-3-030-61010-4_1
Pandey, R., Paul, V., Das, M., Meena, M., & Meena, R. (2017). Plant growth analysis. ResearchGate. https://doi.org/10.13140/RG.2.2.21657.72808
Prestogroup. (n.d.). What is compressive strength? Definition, formula, and applications. https://www.prestogroup.com/articles/what-is-compressive-strength-definition-formula-and-applications/
Sultan, N. F. K., & Johari, W. L. W. (2017). The development of banana peel/corn starch bioplastic film: A preliminary study. Bioremediation Science and Technology Research, 5(1), 12–17. https://doi.org/10.54987/bstr.v5i1.352
Tabasum, S., Younas, M., Zaeem, M. A., Majeed, I., Majeed, M., Noreen, A., Iqbal, M. N., & Zia, K. M. (2018). A review on blending of corn starch with natural and synthetic polymers, and inorganic nanoparticles with mathematical modeling. International Journal of Biological Macromolecules, 122, 969–996. https://doi.org/10.1016/j.ijbiomac.2018.10.092
Tomadoni, B. (2020). Biodegradable materials for planting pots. In Materials research foundations (pp. 85–103). https://doi.org/10.21741/9781644900659-4
Wang, Z., Li, Z., Gu, Z., Hong, Y., & Cheng, L. (2012). Preparation, characterization and properties of starch-based wood adhesive. Carbohydrate Polymers, 88(2), 699–706. https://doi.org/10.1016/j.carbpol.2012.01.023
Zhang, X., Wang, C., & Chen, Y. (2019). Properties of selected biodegradable seedling plug-trays. Scientia Horticulturae, 249, 177–184. https://doi.org/10.1016/j.scienta.2019.01.055
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