DNA Science

The DNA Science Program will introduce students to DNA structure and function, the process of transcribing and translating genetic code into protein, and the study of the human genome. Students will experiment with some of the tools that have allowed scientists to study DNA and the human genome: gel electrophoresis, restriction enzymes, polymerase chain reaction, genetic transformation, and public genome databases. At the end of the course, each student will sequence a small portion of his or her own genome that correlates with a specific physical trait, such as the ability to taste bitter molecules or lactose intolerance.

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The DNA Science Advanced Practicum Program is intended for students who have a passion for hands on modern molecular biology, and perhaps intend to pursue future research level opportunities at the high school or college level. In the world of DNA science it is said that “DNA is the flash, but proteins are the cash.” Students will spend nearly all their time in the Portal at the lab bench using the most up to date techniques of molecular biology to first clone a gene (flash); and then purify the encoded protein (cash). We will use PCR to amplify the amylase gene from Geobacillus stearothermophilus and clone that gene into a DNA expression plasmid. Inserting that plasmid DNA into E. coli bacteria will allow the genetically engineered bacterium to make a new, and commercially viable protein product. This series of techniques lies at the heart of DNA Science and the biotechnology industry.

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