Chromatin Immunoprecipitation
Chromatin Immunoprecipitation
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Chromatin immunoprecipitation, or ChIP, refers to a procedure used to determine whether a given protein binds to or is localized to a specific DNA sequence in vivo. The diagrams below illustrate the basic steps of this procedure.
The diagrams below show a working model of how DNA double strand break induced recombination can occur.
DNA-binding proteins are cross-linked to DNA with formaldehyde in vivo.
Isolate the chromatin. Shear DNA along with bound proteins into small fragments.
Bind antibodies specific to the DNA-binding protein to isolate the complex by precipitation. Reverse the cross-linking to release the DNA and digest the proteins.
Use PCR to amplify specific DNA sequences to see if they were precipitated with the antibody.
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