Purification

Here are the results of your fraction collection efforts!

The photo shows six one dram vials on a lab bench, labeled from left to right: 5, 4, 3, 2, 1, and 6. The “6” vial is positioned beneath the nozzle of a chromatography column with its stopcock in the vertical position. Each vial contains about a two centimeters of clear liquid in the bottom.

Examine the results of your TLC analysis.
The "TP" lane shows the spot from a stock solution of triphenylphosphine oxide.

UV light

The photo shows two thin layer chromatography plates, illuminated bright green by UV light. Each plate has four lane markings on it. The lanes on the first plate are labeled, from left to right: TP, 1, 2, 3. The lanes on the second plate are labeled, from left to right: TP, 4, 5, 6. All eight lanes contain a light gray, vertical oval-shaped spot a short distance above the spotting line. Lanes 1, 2, 3 and 4 also contain larger, darker, rounder spots approximately halfway between the spotting line and the solvent front line.

iodine plate 1

The photo shows the first thin layer chromatography plate, stained off white. The spots visible in the UV visualized photo have been circled in pencil. The spots closer to the spotting line in lanes TP, 1, 2, and 3 are stained dark brown in this photo. A ruler also visible in the photo, with its 0 cm marking aligned with the spotting line, reveals that these dark brown spots are approximately 0.5 cm from the spotting line. The spots farther down the plate in lanes 1, 2, and 3 are strained a lighter brown in this photo. We see from the ruler that these lighter spots appear approximately 2.5 cm from the spotting line.

iodine plate 2

The photo shows the second thin layer chromatography plate, stained off white. The spots visible in the UV visualized photo have been circled in pencil. The spots closer to the spotting line in lanes TP, 4, 5, and 6 are stained dark brown in this photo. A ruler also visible in the photo, with its 0 cm marking aligned with the spotting line, reveals that these dark brown spots are approximately 0.6 cm from the spotting line. The spot farther down the plate in lane 4 is colorless in this photo. We see from the ruler that these lighter spots appear approximately 2.8 cm from the spotting line.

Which fractions would you like to combine in order to isolate your product?

Fractions 1-4

Fractions 5 & 6

"TP" Fractions Only

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