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After you finish collecting fractions, finish up any TLC analysis that you didn't complete while running the column.
The TLC plates you spotted, developed, and visualized under UV and iodine are shown below. The mobile phase used to develop these plates was 10:10:1 toluene : ethyl acetate : acetic acid.
Copy down the plates, making sure to label the compound spotted in each lane of the plate, the solvent used to develop the plate, the solvent front, and how each spot was visualized. Then, calculate Rf values for each spot. For a summary of the TLC data, see the table below.
Note: On Plate 3, the "crude" lane has drifted into lane 9. The table below is constructed assuming that the higher Rf spots "belong" to the crude lane, as it is believed that lane 9 is in fact a pure fraction.
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