MBY-Protein

Eligibility

Category 1: Students currently in B.Sc/M.Sc/Ph.D .

Category 3: Anybody who has finished a Bachelors degree but is not currently enrolled as a student.

The workshop assumes no prior knowledge of molecular biology. Selected participants shall be given introductory reading materials before they come for the workshops.

Fees

Category 1: INR 3000 + 18% GST = INR 3540

Category 3: INR 5000 + 18% GST = INR 5900

How to apply?

  1. You first need to apply by filling up this form . In this form, you should give a preference for the dates of the workshop that you would like to attend. This form will require you to be signed into a Google account. This is because a Google login is mandatory for forms that require uploading of a file. This is a Google policy and has nothing to do with us.

  2. If there are seats available, we will invite you to pay the fees for a given workshop within a stipulated date. Please note that this invitation is strictly on a first-come-first-serve basis (i.e. people who apply earlier, get invited first).

  3. You need to pay the fees within the stipulated date. If you do not, your admission to the workshop stands forfeited and no correspondence will be entertained in this regard.

  4. Once you have paid the fees, please fill up this form and upload the receipt of the fees paid. This form will also require you to be signed into a Google account.

  5. Once we have received your email, we are going to confirm the receipt of the payment. This might take up to 5 working days.

  6. Once we confirm the payment from our side, we are going to write to you with the final confirmation. At this point, your admission to the workshop is confirmed.

Course Content

1. Basic laboratory practices

This will include basic safety precautions, handling of micropipettes and safe disposal of laboratory wastes. To know more about how to handle micropipettes, watch this video or practice using this simulation.

2. Understanding the technique of protein overexpression and extraction

To know more about protein isolation, watch this video.

3.Total protein estimation by spectrophotometry

After the cells have been lysed, the amount of total protein has to be quantified. The simplest way to do this is through the technique of spectrophotometry which detects the concentration of the final reaction product. To know more about spectrophotometry, you can read this article or watch this video for how a spectrophotometer works.

4. SDS-Poly Acrylamide Gel Electrophoresis

Once the protein has been overexpressed, one needs to confirm whether the overexpression has happened or not. One performs the technique of SDS-PAGE to examine the protein expression profile of the samples to separate the proteins in a way similar to agarose gel separation of DNA. To know more about SDS-PAGE, please read this article or watch this video.

5. Transfer of protein and detection using an antibody (Western blotting)

Proteins are transferred from the polyacrylamide gel to a membrane and probed with an antibody specific to the protein of interest. To know more , read this article or watch this video.

6. Enzyme assay to detect activity of protein

The enzyme assay is used to determine the amount of protein activity associated with each sample. The assays are performed in triplicates to gain confidence in the values measured. Enzyme kinetics, read this article or watch this video.