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Zeiss 510 Meta Confocal Laser Scanning Microscope





Contact:
Julie Last
jalast@wisc.edu
608-262-3613




The Materials Science Center has a Zeiss 510 Meta confocal microscope with an Axiovert 200M inverted microscope.   This instrument is equipped with 2 PMT detectors and the META detector.  With the META detector, the emission is projected onto 32 channels for spectral separation. These detectors allow for the simultaneous imaging of several different fluorophores and with the META detector imaging is not limited by the available emission filters.  The microscope has fluorescence capabilities with DAPI, FITC and Rhodamine filter cubes.  DIC imaging is possible with the 20x objective. Confocal images can be taken simultaneously with transmission images.  The microscope is also equipped with a motorized x – y stage. In addition, this system can be easily configured for reflectance confocal to image non-transparent samples. 

Rates: See fees page.  


Instrument details:

Mercury Lamp:

DAPI, FITC and Rhodamine filters


Excitation Sources:

Argon: 458, 477, 488, 514

DPSS: 561

HeNe: 633


Excitation Dichroics: (KP = low pass)

NT 80/20 (passes 80% of the light)

488/561/633

KP 700/488

KP 700/561

458/514

458

488/561

KP 650


Objectives:

1)    A-Plan 10x/0.25air, WD = 4.5  

2)    EC Plan-Neofluar 10x/0.3 air, WD = 5.2

3)    C-Apochromat 10x/0.45 water, WD = 1.8

4)    Plan Apochromat 20x/0.8,WD = 0.55

5)    C-Apochromat 40x/1.2water, WD = 0.28

6)    Fluar 40x/1.3 oil, WD = 0.16

7)    C-Apochromat 63x/1.2 water, WD = 0.28

8)    Plan-Apochromat 63x/1.4 oil, WD = 0.19


Emission filters: (KP = low pass, BP = band pass, LP = high pass)

Channel S: 32 channel META detector

Channel 2: KP 685, BP 390 – 465, BP 435 – 485, BP 480 – 520, BP 500 – 530, BP 500 – 550

Channel 3: LP 505, BP 510/20, BP 500 – 550, BP 535 – 590, BP 575 – 615, BP 650 – 710



Other features:

DIC imaging possible with the 20x objective

Reflectance confocal                                                                                                              

Automated x – y stage

Pinholes for each detection channel can be individually adjusted

 

Links:

Zeiss LSM image browser  

Image J with LSM reader 

SpectraViewer


Detailed specifications

Manufacturer's brochure