Principal Investigator: Dr. Kuo-Chih Liao

Education

Ph.D., Biomedical Engineering, University of Southern California, USA, 2006

M.S., Biochemistry and Molecular Biology, National Taiwan University, Taiwan, 1997

B.S., Chemical Engineering, National Taiwan University, Taiwan, 1995

 

Professional Experience

2016-Present  Associate Professor, Graduate Institute of Biomedical Engineering, National Chung    
                        Hsing University, Taiwan
2008-2016       Assistant Professor, Graduate Institute of Biomedical Engineering, National Chung    
                        Hsing University, Taiwan
2006-2007       Postdoctoral Fellow, Department of Biomedical Engineering, University of California
                        Davis, USA (w. Dr. Katherine W. Ferrara and Dr. Laura Marcu)
2002-2006       Research Assistant, Medical Device Development Facility, Alfred Mann Institute, USA
1999-2001       Research Engineer, Taiwan Biotech Co., LTD., Taiwan 

 

Research Interest  

Biographical Sketch

         The main research focus of Principal Investigator can be classified into two categories: firstly, development of a fiber-optic platform, which is disposable, minimally invasive, and can provide simultaneously in vivo monitoring of various analytes up to several weeks [1,2,4-7,9,10,11] and in vivo personalized (tunable) delivery of therapeutic agents [3]; secondly, designs of contrast agents of medical imaging for clinical conditions, lesion malignancy screening [5,12] and contrast media induced nephropathy prevention [5].  
          The biocompatibility of the fiber-optic sensor platform was verified in animal models, and the fabrication procedure was optimized for the capability in industrial mass scale production [7,9,10]. The 1st application of the sensor platform is a glucose sensor, which can be integrated into an “artificial pancreas” consisting of an insulin pump and close-loop algorithm as the signal input of the feedback control. The preliminary results of accuracy agreement analysis from canine study implied the potential of the sensor for clinical applications [4]. The 2nd application is to trigger in vivo topical release of photo-thermal responsive liposome (incorporation of hydrophobic gold nanoparticles in a temperature-responsive liposome) [3]. The therapeutic effect from the vesicle delivering anti-cancer agent can by quantified immediately with the scrambling of membrane phosphatidylserine in apoptotic cancer cells by the fiber-optic platform, aiming as the personalized chemotherapy system [1,2,6]. The immediate evaluation of chemotherapy effect by early apoptotic reaction has the potential for significant clinical benefits in improving efficacy and reducing adverse events.
       For improving lesion diagnosis with medical imaging, we established a xenograft model demonstrating essential clinical properties of malignant and benign lesions [12]. The animal model was applied in characterizing the diagnosis outcomes of lesion malignancy screening and delineation from several customized contrast agents. diglucosamid-SIDAG, a custom-made NIR fluorophore, exhibited “delayed wash-out contrast” in cancer tissue, which can be applied in lesion malignancy indication and delineation of tumor margin. The encapsulation of conventional CT (computed tomography scan) contrast agent in long-circulating liposome was found to improve the diagnosis accuracy and specificity in the animal model and could also eliminate the contrast media induced nephropathy [5].

Publication
1.   K.C. Liao*, H.S. Chiu, S.Y. Fan, Y.T. Zeng, P.H. Lu. A percutaneous fiber-optic biosensor for the 
      immediate evaluation of chemotherapy efficacy in vivo (part I): strategy of assay design for
      monitoring non-homogeneously distributed biomarkers. Sensors and Actuator B: Chemical, 222,
      544-550, 2016.
(SCI, IF = 5.401@JCR2016, ranking 2/58 or 3.45% in Instruments and Instrumentation,
NSC 100-2221-E-005-032)


2.   S.Y. Fan, H.S. Chiu, T.Y. Lin, A. Liao, K.C. Liao*. Percutaneous fiber optic biosensor for
      immediate evaluation of chemotherapy efficacy in vivo (part II): In vitro and in vivo
      characterization. Sensors and  Actuator B: Chemical, 222, 579-587, 2016.
(SCI, IF = 5.401@JCR2016, ranking 2/58 or 3.45% in Instruments and Instrumentation,
NSC 100-2221-E-005-032)
      

3.   H.L. Huang, P.H. Lu, H.C. Yang, G.D. Lee, H.R. Li, K.C. Liao*. Fiber-optic triggered release of
      liposome in vivo: implication of personalized chemotherapy. International Journal of
      Nanomedicine, 10, 5171-5185, 2015.
(SCI, IF = 4.300@JCR2016, ranking 37/256 or 14.26% in Pharmacology and Pharmacy,
NSC 97-2218-E-005-096)

 

4.   K.C. Liao*, S.C. Chang, C.Y. Chiu, Y.H. Chou. Acute response in vivo of a fiber-optic sensor for 
      continuous glucose monitoring from canine studies on point accuracy. Sensors, 10, 7789-7802,
      2010.
(SCI, IF = 2.677@JCR 2016, ranking 10/58 or 16.38% in Instruments and Instrumentation,
NSC 98-2221-E-005-001)

 

      

5.   G.D. Lee, J.W. Chai, L.C. Hu, P.H. Lu, K.C. Liao*. Influence of liposome encapsulation on lesion
      malignancy screening and delineation in dynamic computed tomography. International Journal of
      Bioscience, Biochemistry and Bioinformatics, 3, 562-565, 2013.

(TCVGH-NCHU 1017611)

 

        

6.   J. O’Kelly, K.C. Liao*, W. Clifton, D. Lu, P. Koeffler, G. Loeb. Percutaneous fiber-optic sensor for
      the detection of chemotherapy-induced apoptosis in vivo. Proceedings of SPIE, 7555, 75551H,
      2010.

(EI)

 


7.   K.C. Liao, T. Hogen-Esch, F.J. Richmond, L. Marcu, W. Clifton, G.E. Loeb*. Percutaneous fiber-
      optic sensor for chronic glucose monitoring in vivo. Biosensors and Bioelectronics, 23, 1458-1465,
      2008.

(SCI, IF = 7.780@JCR2016, ranking 1/29 or 3.44% in Electrochemistry)

 


8.   Y. Sun, K.C. Liao, Y. Sun, J. Park, L. Marcu*, Novel tissue phantom for testing a dual-modality
      diagnostic system: time-resolved fluorescence spectroscopy and high frequency ultrasound.
      Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements
      of Tissue, Proceedings of SPIE 6870, 68700D, 2008.

(EI)

 


9.   K.C. Liao, T. Hogen-Esch, F.J. Richmond, L. Marcu, W. Clifton, G.E. Loeb*, Design and
      fabrication of a disposable, percutaneous glucose sensors. Optical Fibers and Sensors for
      Medical Diagnostic and Treatment VI, Proceedings of SPIE 6083, 60830V, 2006.

(EI)

        
        
10.   K.C. Liao, T. Hogen-Esch, F.J. Richmond, L. Marcu, G.E. Loeb*. Design and fabrication of
       disposable, percutaneous chemical sensors. Optical Fibers and Sensors for Medical Application
       V, Proceedings of SPIE 5691, 129-145, 2005.
 (EI)
 

11.  K.C. Liao, F.J. Richmond, T. Hogen-Esch, L. Marcu, G.E. Loeb, Sencil ProjectTM: Development
       of a percutaneous optical biosensor. Proceeding of the 26th Annual International Conference of
       the IEEE EMBS, 2082-2085, 2004.
 
12.   T.H. Yen, G.D. Lee, J.W. Chai, J.W. Liao, J.Y. Lau, L.C. Hu, K.C. Liao*. Characterization of
        a murine xenograft model for contrast agent development in breast lesion malignancy
        assessment. Journal of Biomedical Science, 23:46 (13 pages), 2016.
       (SCI, IF = 2.799@JCR 2016, ranking 51/128 or 39.84% in Medicine, Research & Experimental 
       NSC 99-2221-E-005-026)