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Biosensors Lab (CHIM/01-CHIM/02-MED46)

Head of Laboratory: MAZZEI Franco
Website: Surf the link


Main research topics:
The main areas of interest of Biosensors Laboratory are:
1) Nanostructured and supramolecular materials for the development of optical and electrochemical biosensors;
2) Development of Enzymatic Biofuel Cells modified with conducting polymers and nanostructured materials;
3) Study of the electrochemical properties of modified conducting polymers in the development of electron transfer based biosensors by means of both electrochemical and spectroelectrochemical instruments;
4) Evaluation and optimization of the different immobilization procedures of enzymes and mediators, that can be used in the development of enzymatic screen printed electrodes;
5) Application of Electrochemical Surface Plasmon Resonance (ESPR) in the evaluation of the growth of proteins, mediators and self-assembled monolayers (SAMs) on gold surfaces;
6) Development of ESPR and Electrochemical Impedance Spectroscopy based immunosensors and their application in clinical, toxicological and pharmacological fields.

National and International Collaborations:
One of the most important peculiarities of the research carried out in the Biosensors Lab is represented by its multidisciplinarity, thanks also to several partnerships established over the years with national and international research groups: Salvatore Cannistraro of the Biophysics & Nanoscience Center & CNISM Faculty of Sciences, University of Tuscia, Viterbo; Fred Lisdat Biosystems Technology, Institute for Applied Life Sciences, Technical University of Applied Sciences Wildau, GermanyHarry Boer, Anu Koivula, Maria Smolander - VTT Technical Research Center of Finland (Finland); Oded Shoseyov - The Hebrew University of Jerusalem (Israel); David Hernandez Santos, Pablo Fanjul Bolado, DropSens (Spain).

Facilities:
Biosensors Lab facilities consists of several electrochemical interfaces, two electrochemical Impedance spectroscopy instruments, one spectroelectrochemical interface, Surface Plasmon Reosnance instruments, spectrophotometer, flow injection analysis systems. In addition, other scientific instrumentations such as: ESI mass spectrometer, Atomic force microscopy, Scanning tunneling microscope, scanning electron microscope (SEM) for surface characterization are available.

ERC panels:
PE4_1 - Physical chemistry PE4_11 - Physical chemistry of biological systems
PE4_4 - Surface science and nanostructures
PE4_5 - Analytical chemistry
PE4_8 - Electrochemistry, electrodialysis, microfluidics, sensors

Grants (2015-2018):
1) Research contract with Dianax S.r.l. about the electrochemical characterization of selected organic molecules.
2) Research contract with Seleo Engineering S.r.l. about the development of a biosensor-based portable electrochemical device for real samples analysis
3) Research contract with Dianax S.r.l. about the develop’0ment and characterization of electrochemical cell integrated with microfluidic devices for diagnosis applications.

Laboratory Members:
Favero Gabriele (AP)
Antiochia Riccarda (AP)
Zumpano Rosaceleste (PhD student)

Publications (2015-2018):
1) Fusco, G., Göbel, G., Zanoni, R., Bracciale, M.P., Favero, G., Mazzei, F., Lisdat, F.
Aqueous polythiophene electrosynthesis: A new route to an efficient electrode coupling of PQQ-dependent glucose dehydrogenase for sensing and bioenergetic applications
(2018) Biosensors and Bioelectronics, 112, pp. 8-17.
DOI: 10.1016/j.bios.2018.04.014

2) Fusco, G., Chronopoulou, L., Galantini, L., Zerillo, A., Rasik, Z.M., Antiochia, R., Favero, G., D'Annibale, A., Palocci, C., Mazzei, F.
Evaluation of novel Fmoc-tripeptide based hydrogels as immobilization supports for electrochemical biosensors
(2018) Microchemical Journal, 137, pp. 105-110.
DOI: 10.1016/j.microc.2017.10.002

3) Bollella, P., Fusco, G., Stevar, D., Gorton, L., Ludwig, R., Ma, S., Boer, H., Koivula, A., Tortolini, C., Favero, G., Antiochia, R., Mazzei, F.
A Glucose/Oxygen Enzymatic Fuel Cell based on Gold Nanoparticles modified Graphene Screen-Printed Electrode. Proof-of-Concept in Human Saliva
(2018) Sensors and Actuators, B: Chemical, 256, pp. 921-930.
DOI: 10.1016/j.snb.2017.10.025

4) Tortolini, C., Favero, G., Mazzei, F.
Development of amine-oxidase-based biosensors for spermine and spermidine analysis
(2018) Methods in Molecular Biology, 1694, pp. 75-80.
DOI: 10.1007/978-1-4939-7398-9_7

5) Tomassetti, M., Merola, G., Martini, E., Campanella, L., Sammartino, M.P., Sanzò, G., Favero, G., Mazzei, F.
Ampicillin measurement using flow SPR immunosensor and comparison with classical amperometric immunosensor
(2018) Lecture Notes in Electrical Engineering, 431, pp. 229-232.
DOI: 10.1007/978-3-319-55077-0_30

6) Sanzò, G., Taurino, I., Puppo, F., Antiochia, R., Gorton, L., Favero, G., Mazzei, F., Carrara, S., De Micheli, G.
A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H2O2 detection at low potential
(2017) Methods, 129, pp. 89-95.
DOI: 10.1016/j.ymeth.2017.06.005

7) Fusco, G., Göbel, G., Zanoni, R., Kornejew, E., Favero, G., Mazzei, F., Lisdat, F.
Polymer-supported electron transfer of PQQ-dependent glucose dehydrogenase at carbon nanotubes modified by electropolymerized polythiophene copolymers
(2017) Electrochimica Acta, 248, pp. 64-74.
DOI: 10.1016/j.electacta.2017.07.105

8) Di Turo, F., De Vito, C., Coletti, F., Mazzei, F., Antiochia, R., Favero, G.
A multi-analytical approach for the validation of a jellified electrolyte: Application to the study of ancient bronze patina
(2017) Microchemical Journal, 134, pp. 154-163.
DOI: 10.1016/j.microc.2017.05.015

9) Fusco, G., Gallo, F., Tortolini, C., Bollella, P., Ietto, F., De Mico, A., D'Annibale, A., Antiochia, R., Favero, G., Mazzei, F.
AuNPs-functionalized PANABA-MWCNTs nanocomposite-based impedimetric immunosensor for 2,4-dichlorophenoxy acetic acid detection
(2017) Biosensors and Bioelectronics, 93, pp. 52-56.
DOI: 10.1016/j.bios.2016.10.016

10) Tomassetti, M., Merola, G., Martini, E., Campanella, L., Sanzò, G., Favero, G., Mazzei, F.
Comparison between a direct-flow SPR immunosensor for ampicillin and a competitive conventional amperometric device: Analytical features and possible applications to real samples
(2017) Sensors (Switzerland), 17 (4), art. no. 819, .
DOI: 10.3390/s17040819

11) Bollella, P., Mazzei, F., Favero, G., Fusco, G., Ludwig, R., Gorton, L., Antiochia, R.
Improved DET communication between cellobiose dehydrogenase and a gold electrode modified with a rigid self-assembled monolayer and green metal nanoparticles: The role of an ordered nanostructuration
(2017) Biosensors and Bioelectronics, 88, pp. 196-203.
DOI: 10.1016/j.bios.2016.08.027

12) Taurino, I., Sanzó, G., Carrara, S., De Micheli, G., Favero, G., Mazzei, F., Antiochia, R.
One-step rapid synthesis of Au-Pt nanofems for electrochemical sensing and biosensing
(2017) Proceedings of IEEE Sensors, art. no. 7808406, .
DOI: 10.1109/ICSENS.2016.7808406

13) Tortolini, C., Sanzò, G., Antiochia, R., Mazzei, F., Favero, G.
Application of a nanostructured enzymatic biosensor based on fullerene and gold nanoparticles to polyphenol detection
(2017) Methods in Molecular Biology, 1572, pp. 41-53.
DOI: 10.1007/978-1-4939-6911-1_4

14) Bollella, P., Schulz, C., Favero, G., Mazzei, F., Ludwig, R., Gorton, L., Antiochia, R.
Green Synthesis and Characterization of Gold and Silver Nanoparticles and their Application for Development of a Third Generation Lactose Biosensor
(2017) Electroanalysis, 29 (1), pp. 77-86.
DOI: 10.1002/elan.201600476

15) Sanzó, G., Taurino, I., Antiochia, R., Gorton, L., Favero, G., Mazzei, F., De Micheli, G., Carrara, S.
Bubble electrodeposition of gold porous nanocorals for the enzymatic and non-enzymatic detection of glucose
(2016) Bioelectrochemistry, 112, pp. 125-131.
DOI: 10.1016/j.bioelechem.2016.02.012

16) Guizzo, S., Tortolini, C., Pepi, F., Leonelli, F., Mazzei, F., Di Turo, F., Favero, G.
Application of microemulsions for the removal of synthetic resins from paintings on canvas
(2016) Natural Product Research, pp. 1-11. Article in Press.
DOI: 10.1080/14786419.2016.1244191

17) Tomassetti, M., Conta, G., Campanella, L., Favero, G., Sanzò, G., Mazzei, F., Antiochia, R.
A flow SPR immunosensor based on a sandwich direct method
(2016) Biosensors, 6 (2), art. no. 22, .
DOI: 10.3390/bios6020022

18) Bollella, P., Fusco, G., Tortolini, C., Sanzò, G., Antiochia, R., Favero, G., Mazzei, F.
Inhibition-based first-generation electrochemical biosensors: Theoretical aspects and application to 2,4-dichlorophenoxy acetic acid detection
(2016) Analytical and Bioanalytical Chemistry, 408 (12), pp. 3203-3211.
DOI: 10.1007/s00216-016-9389-z

19) Taurino, I., Sanzò, G., Antiochia, R., Tortolini, C., Mazzei, F., Favero, G., De Micheli, G., Carrara, S.
Recent advances in Third Generation Biosensors based on Au and Pt Nanostructured Electrodes
(2016) TrAC - Trends in Analytical Chemistry, 79, pp. 151-159.
DOI: 10.1016/j.trac.2016.01.020

20) Antiochia, R., Bollella, P., Favero, G., Mazzei, F.
Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors for in Vitro Diagnostics
(2016) International Journal of Analytical Chemistry, 2016, art. no. 2981931, .
DOI: 10.1155/2016/2981931

21) Fusco, G., Bollella, P., Mazzei, F., Favero, G., Antiochia, R., Tortolini, C.
Catalase-based modified graphite electrode for hydrogen peroxide detection in different beverages
(2016) Journal of Analytical Methods in Chemistry, 2016, art. no. 8174913, .
DOI: 10.1155/2016/8174913

22) Favero, G., Fusco, G., Mazzei, F., Tasca, F., Antiochia, R.
Electrochemical characterization of graphene and MWCNT screen-printed electrodes modified with AuNPs for laccase biosensor development
(2015) Nanomaterials, 5 (4), pp. 1995-2006.
DOI: 10.3390/nano5041995

23) Tortolini, C., Bollella, P., Antiochia, R., Favero, G., Mazzei, F.
Inhibition-based biosensor for atrazine detection
(2015) Sensors and Actuators, B: Chemical, 224, pp. 552-558.
DOI: 10.1016/j.snb.2015.10.095

24) Taurino, I., Sanzó, G., Mazzei, F., Favero, G., De Micheli, G., Carrara, S.
Fast synthesis of platinum nanopetals and nanospheres for highly-sensitive non-enzymatic detection of glucose and selective sensing of ions
(2015) Scientific Reports, 5, art. no. 15277, .
DOI: 10.1038/srep15277

25) Tortolini, C., Bollella, P., Antonelli, M.L., Antiochia, R., Mazzei, F., Favero, G.
DNA-based biosensors for Hg2+ determination by polythymine-methylene blue modified electrodes
(2015) Biosensors and Bioelectronics, 67, pp. 524-531.
DOI: 10.1016/j.bios.2014.09.031

26) Sanzó, G., Tortolini, C., Antiochia, R., Favero, G., Mazzei, F.
Development of carbon-based nano-composite materials for direct electron transfer based biosensors
(2015) Journal of Nanoscience and Nanotechnology, 15 (5), pp. 3423-3428.
DOI: 10.1166/jnn.2015.10207

27) Tomassetti, M., Martini, E., Campanella, L., Favero, G., Sanzó, G., Mazzei, F.
A new surface plasmon resonance immunosensor for triazine pesticide determination in bovine milk: A comparison with conventional amperometric and screen-printed immunodevices
(2015) Sensors (Switzerland), 15 (5), pp. 10255-10270.
DOI: 10.3390/s150510255

28) Boffi, A., Favero, G., Federico, R., Macone, A., Antiochia, R., Tortolini, C., Sanzó, G., Mazzei, F.
Amine oxidase-based biosensors for spermine and spermidine determination
(2015) Analytical and Bioanalytical Chemistry, 407 (4), art. no. 8324, pp. 1131-1137.
DOI: 10.1007/s00216-014-8324-4

29) Antiochia, R., Favero, G., Conti, M.E., Mazzei, F., Tortolini, C.
Affinity-based biosensors for pathogenic bacteria detection
(2015) International Journal of Environmental Technology and Management, 18 (3), pp. 185-206.
DOI: 10.1504/IJETM.2015.071140

30) Tomassetti, M., Martini, E., Campanella, L., Favero, G., Sanzò, G., Mazzei, F.
Atrazine determination using immunosensor method based on surface plasmon resonance. Comparison with two other immunological methods based on screen-printed and classical amperometric devices
(2015) Lecture Notes in Electrical Engineering, 319, pp. 65-69.
DOI: 10.1007/978-3-319-09617-9_12

31) Mazzei, F., Favero, G., Bollella, P., Tortolini, C., Mannina, L., Conti, M.E., Antiochia, R.
Recent trends in electrochemical nanobiosensors for environmental analysis
(2015) International Journal of Environment and Health, 7 (3), pp. 267-291.
DOI: 10.1504/IJENVH.2015.073210

32) Sanzo, G., Taurino, I., De Micheli, G., Carrara, S., Favero, G., Mazzei, F.
Highly sensitive electrodic materials based on Pt nanoflowers grown on Pt nanospheres for biosensor development
(2015) IEEE-NANO 2015 - 15th International Conference on Nanotechnology, art. no. 7388927, pp. 1501-1504.
DOI: 10.1109/NANO.2015.7388927