I am having a problem with Trinity, I think I am doing everything right.
I have a single end read RNA seq library in FASTQ format, which I ran through the FASTQ groomer.
When I try to run Trinity on the groomed data set, I get the following in the log:
Error, cannot find meryl at /soft/trinityrnaseq/20110820/bin/trinity-plugins/kmer/meryl/meryl at /soft/trinityrnaseq/20110820/bin/Trinity.pl line 194.
And there is no data in the Assembled transcript.
It looks to me like the program can't find the place it needs to run, but I could also be missing a setting.
Thanks for any help,
Data is single end,
Strand specific library is F or none (neither work)
No additional parameters
Here are the first few lines of the groomed FASTQ file: