Center for Live Cell Imaging - CLCI

The Center for Live-Cell Imaging (CLCI) was created in 2005 with the goal of becoming a university wide resource providing access to the tools and expertise required for quantitative fluorescence microscopy and image analysis in living cells.

The CLCI offers access to, and training on, five microscopes designed for general to specialized fluorescence microscopy techniques catered to your level of microscopy experience.

We also offer expert training and custom software creation for image analysis on Metamorph, ImageJ, and Huygens; three extraordinarily powerful image processing programs.

In addition we offer consultation with experimental design or data analysis in:

  • Wide-field and confocal fluorescence

  • Live cell fluorescence

  • 3D imaging and deconvolution

  • Forster Resonance Energy Transfer (FRET) Stoichiometry

  • Ratiometric imaging

If you are interested in using any of the microscopes, processing computers, or consultation services we offer, please fill out a new user form, and contact

Sei Yoshida or Matangi Marthi at

about setting up a time to meet.

The CLCI is located in Medical Sciences II, room 5631, (map).

We have Huygens image processing and deconvolution software! We also have a great Online Nyquist calculator