Wei-Lung Tseng / 曾韋龍





Office/Lab: C3007, Departments of Chemistry, National Sun Yat-sen University

                  國立中山大學化學系C3007

 

Tel: +886-7-5252000 ext. 3925

Fax: +886-07-5254644

E-mail: tsengwl@mail.nsysu.edu.tw


 

 

Experience / 經歷


2015-present: Chair , Departmet of Chemistry, National Sun Yat-sen Univeristy / 國立中山大學化學系 系主任 

2013-present: Professor, Departmet of Chemistry, National Sun Yat-sen Univeristy / 國立中山大學化學系 教授 

2009-2012: Associate Professor, Departmet of Chemistry, National Sun Yat-sen Univeristy / 國立中山大學化學系 副教授

2005-2008: Assistant Professor, Departmet of Chemistry, National Sun Yat-sen Univeristy / 國立中山大學化學系 助理教授


2004-2005, Postdoctoral researcher / 博士後研究

Department of Chemistry, National Taiwan University / 國立台灣大學化學系

Research advisors: Prof. Huan-Tsung Chang / 指導教授張煥宗 教授


 

Education / 學歷


2002Ph. D., Department of Chemistry, National Taiwan University / 國立台灣大學化學系博士

Thesis: Discontinuous system on capillary and microchip electrophoresis for high-sensitivity bioassay

Research advisors: Prof. Huan-Tsung Chang 指導教授張煥宗 教授 

1997, B. S., Department of Chemistry, Fu-Jen Catholic University輔仁大學化學系學士



Honer / 學術榮譽


國立台灣大學第十一屆顏氏博士論文獎 (2002)

國立台灣大學理學院研究所院長獎 (2002)

國立中山大學研究績優獎(年輕學者獎)(2009) / National Sun Yat-sen University Outstanding Research Award for Young Scholar, 2009  

中國化學會傑出青年化學獎 (2009) / Chinese Chemical Society Outstanding Young Chemist Award, 2009

國科會100年度吳大猷先生紀念獎 (2011) / Mr. Wu Ta-you Memorial Award, National Science Council, Taiwan

國立中山大學特聘年輕學者 (2011)

國立中山大學特聘研究學者(2013-2014)

第十六屆水木化學文教基金會傑出青年學者獎(2015)


Research interest / 研究興趣


Capillary electrophoresis:

(a) Development of novel method for separation of biomolecules

(b) Development of stacking techniques in capillary electrophoresis

(c) Imaging system in conjugated with capillary electrophoresis


Nanoscience

(a) Synthesis of novel nanomaterials

(b) Development of novel biosensor based on nanomaterials

(c) The use of nanomaterials as pseudostationary phase in capillary electrophoresis

(d) The use of nanomaterials as inorganic matrix in laser desorption/ionization mass spectrometry

(e) The use of nanomaterials as extracting agent

(f) The use of nanomaterials as mimic enzyme



Research Grants / 研究計畫


1. Ministry of science and technology, 透過費頓反應蝕刻奈米材料產生螢光奈米材料:金奈米簇、聚多巴胺量子點、石墨烯量子點principal investigator,                     2015/08/01-2018/07/31, award number 104-2113-M-110-004-MY3


2National science council, 功能性奈米粒子選擇性萃取與濃縮體液內生物分子並結合毛細管電泳分離” , principal investigator, 
    2009/08/01-2012/07/31, award number 98-2113-M-110-009-MY3.


3. National science council, “氧化鐵奈米粒子在感測器、萃取劑與去除劑之應用”, principal investigator, 2011/08/01-2015/07/31,
    award number
 100-2628-M-110-001-MY4.


4. Ministry of science and technology, “延攬人才(博士後研究)-林佳慧”, principal investigator, 2015/08/01-2016/07/31, award number 104-2811-M-110 -022-


5. Ministry of science and technology, “大專生參與專題研究計畫(李金翰)”, principal investigator, 2015/07/01~2016/02/28, award number 104-2815-C-110-046-M.

 



Research experience.   

2005-to date. Wei-Lung Tseng is currently a Professor of Chemistry at National Sun Yet-sen University. He began his independent career as a member of the chemistry faculty at National Sun Yet-sen University in August of 2005. He has authored and co-authored more than 80 scientific publications. His research group is creating structures on metal nanoparticles for the development of colorimetric/fluorescent sensors and nanoparticle-related extraction system based molecular recognition and covalent bonding formation. Moreover, he devised a series of oligonucleotide-based sensors for selective and sensitive detection of heavy metal ions, anti-cancer drug, and single nucleotide polymorphisms.  Current research includes: (i) the stabilization of gold nanoparticles and iron oxide nanoparticles with specific ligands for colorimetric/fluorescent detection of heavy metal ions, aminothiols, cyanide, hydrogen peroxide, and enzyme activity;  (ii) protein-directed synthesis of highly fluorescent metal nanocluster and its application to the detection of Hg(II), CH3Hg(I), and glutathione; (iii) the combination of nanoparticle-based extraction and capillary electrophoresis for the determination of thiol-containing  molecules, catechol-containing molecules, and environmental pollutants; and (iv) the development of oligonucleotide-related sensors based on non-base pairing interaction.

2004-2005. Wei-Lung Tseng joined the laboratory of Professor Huan-Tsung Chang (National Taiwan University, Taiwan) as a postdoctoral fellow. During this time, he developed whole-column imaging capillary electrophoresis for monitoring the migration of long DNA molecules, constructed a fluorescence and scattering microscopy system for determining the distribution and concentrations of glutathione within erythrocytes, and used a layer-by-layer assembly technique for fabricating multicolored-light-emitting films of CdSe@CdS and CdTe quantum dots.

1997-2002. Wei-Lung Tseng began his studies in Chemistry at National Taiwan University in 1997 and received his Ph.D. degree from National Taiwan University under the supervisor of Professor Huan-Tsung Chang in 2002. He explored how to use polymer solution to control electroosmotic flow in capillary electrophoresis. This finding opens a range of applications where polymer solutions are used to separate small molecules, DNA fragments, and proteins in the presence of electroosmotic flow. He won the "Dean's Award" Ph.D. degree in College of Science, National Taiwan University in 2002.


Research contribution

Wei-Lung Tseng’s research encompasses wide areas concerned with the synthesis and application of nanoparticles in biosensors, capillary electrophoresis, surface-assisted laser desorption/ionization time-of-flight mass spectrometry, catalysis, and extraction. Moreover, he designed a series of oligonucleotides to be used as molecular beacons and optical sensors based on non-watson crick base pairing. The work traverses areas of analytical chemistry, material chemistry, and more recently bio-analytical and environmental chemistry. He has demonstrated the practical applicability of these different types of sensors for detecting heavy metal ions, aminothiols, hydrogen peroxide, cyanide, enzyme activity, single nucleotide polymorphisms and anti-cancer drug coralyne. Dr. Tseng has authored and co-authored over 80 published articles in peer-reviewed scientific journals with more than 1200 citations. The h index of 25 after 11 years of scientific activity is a clear indication of his consistent publication productivity behavior. Some of the recent highlights of the research are as follows:


1. Gold nanoparticle-based sensors: Dr. Tseng conducted a label-free, rapid, and homogeneous method for sensing both Hg(II) and Ag(I) using Tween 20-stabilized gold nanoparticles (Anal. Chem. 2010, Vol. 82, pp. 6830). Citrate ions were found to still be adsorbed on the Au surface when citrate-capped gold nanoparticles were modified with Tween 20. When citrate ions had reduced Hg(II) and Ag(I) to form Hg-Au alloys and Ag on the surface of the gold nanoparticles, Tween 20 was removed from the nanoparticle surface. As a result, the gold nanoparticles were unstable under a high-ionic-strength solution, resulting in nanoparticle aggregation. This work is highly cited and opens a range of applications where surfactant-stabilized gold nanoparticles are capable of sensing cysteine, homocysteine, S-adenosylhomocysteine hydrolase, adenosine, cyanide, and cyanogenic glycoside-containing plants.


2. Polyadenosine-coralyne interaction: Dr. Tseng created a simple, sensitive, and selective-detection system for coralyne, polyadenylation reaction, and single nucleotide polymorphisms based on the fact that coralyne induces conformational change of polyadenosine oligonucleotide through adenosine2-coralyne-adenosine2 coordination (Chem. Commun. 2011, Vol. 47, pp. 11134; Chem. Commun. 2011, Vol. 48, pp. 6262). This is first example for designing molecular beacon probe using the adenosine2-coralyne-adenosine2 structure. In contrast to base pairing molecular beacon, the proposed molecular beacon can be used for discriminating between perfect matched DNA and single mismatched DNA at room temperature without the interferences of DNase I and single-stranded DNA-binding proteins.


3. Protein-directed synthesis of gold nanoclusters: Dr. Tseng demonstrated a straightforward one-pot approach to synthesize high fluorescent Au8 clusters (Quantum yield 56%) and Au25 clusters (Quantum yield 9%) with lysozyme type VI (Anal. Chem. 2010, Vol. 82, pp. 9194; Small 2012, Vol. 8, pp. 1912). Dr. Tseng revealed that lysozyme type VI provides a relatively small internal space for synthesizing Au8 clusters at pH 3.0, while serving as a relatively large template for preparing Au25 clusters at pH 12.0. The observation of Au8 clusters is helpful in understanding the protein-mediated formation of Au25 clusters. The obtained Au8 and Au25 clusters provided the first example for detecting glutathione and CH3Hg(I), respectively. 


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