Our research is focused on determining the optimal uterine conditions for embryo implantation, using the mink as a biological model.
The Rose Lab is interested in understanding the hormonal regulation of glycogen breakdown and mobilization of glucose in the uterus of mink. Glycogen is an important energy source for a developing embryo, and its concentrations are shown to fluctuate based on the reproductive cycle across several species. In mink, rat and humans, glycogen concentrations decrease near the time of implantation, suggesting the involvement of glycogen in implantation. In humans, diminished or complete absence of uterine glycogen is seen in women who are sterile or have experiencedspontaneous abortions; again, this demonstrates the importance of glycogen in early pregnancy. In addition to being an energy source, findings suggest glucose (from glycogen) may act as a signal to increase gene expression of genes necessary for continued development of the blastocyst. Our lab is interested in discovering what is happening to glycogen/glucose at the time of implantation, the time leading up to implantation (peri-implantation) and after implantation (post-implantation) in hopes to find ways to increase the rate of successful pregnancies in humans and other species.
Our laboratory investigates how uterine glycogen and metabolizing enzymes change after mating and how these enzymes are regulated by a variety of hormones. We use the American Mink (Neovison vison) as a model organism due to its unique implantation type (see our Lab Techniques page for further discussion). We are interested in seeing how different hormones, such as estrogens, prostaglandins, and progesterone, are involved in glycogen metabolism. There are several techniques that we use in our lab to help us learn more about key enzymes involved in these processes.
Currently, we are looking at the enzymes hexokinase, glucose-6-phosphatase, glycogen synthase, glycogen phosphorylase, and glycogen synthase kinase-3 beta. By comparing both protein and gene expression levels, we can discover whether regulation of specific enzymes is occurring pre- or post-translationally.
Primary Investigator: Dr. Jack Rose
The Rose Lab is located in Gale Life Science 307. Directions: google map, ISU campus map
Idaho State University Construction-