β-catenin is a potent oncogene that is deregulated in many cancers. This investigation studied the mechanism underlying the powerful biochemical modificaton, O-GlcNAcylation, and its regulatory role in suppressing β-catenin’s oncogenic properties. The proposed hypotheses were supported experimentally and are summarized below:
Hypotheses and Summary of Supporting Data
An established working knowledge of O-GlcNAc modifications involved in β-catenin localization will allow us to better understand the regulation of this powerful oncoprotein, and provide exciting possibilities for diagnostic and therapeutics.
Possible Applications and Significance
This particular study investigated the molecular mechanisms underlying the O-GlcNAcylation of β-catenin. This report lacked experiments that explore the biological impact of O-GlcNAc-β-catenin. Cell invasion assays and/or migration assays could be performed to assess the degree of cell invasiveness and transformation upon increased O-GlcNAcylation. Furthermore, other sites of O-GlcNAcylation remain to be elucidated.