Acknowledgements

Materials, Equipment and Facilities

I would like to thank Dr. Sujata Persad for providing access to all of the laboratory reagents, cell culture and equipment. Special thank you to Dr. Baksh for providing the U2OS/SAOS2 Osteosarcoma cell lines.

Mentorship and Guidance

I would like to thank Dr. Sujata Persad for providing essential background knowledge of β-catenin, tumorigenesis and signaling pathways. She routinely met with me to discuss my results and experimental pitfalls. The constant feedback she offered was instrumental to the completion of this study.

Project Assistance

I would like to thank my Sanofi-Aventis Biotalent Challenge (SABC) and Canada Wide Science Fair (CWSF) partner, Jaclynn Wong, for her assistance of certain experiments. I would also like to thank all personnel of the Persad Laboratory for the preparation of buffers, reagents and equipment set-up as well as for the assistance of equipment operation, including confocal microscopy, luminometer and PCR instruments. 

Timeline

This project was performed under the supervision of Dr. Sujata Persad from the summer of 2010 to present.

Project Photos

All diagrams and illustrations included within this project were either drawn, waived of copyright or obtained with explicit permission from the authors. Special thank you to Dr. Vocadlo and Dr. Davies for providing the 3D rendering of the enzyme O-GlcNAcase included in the Conclusion section.

Student Funding

I would like to thank Heritage Youth Researcher Summer (HYRS) Program for not only providing a research stipend, but also for opening doors of opportunities. 

I would like to acknowledge the J.R. Paine Endowment Fund, which provided a stipend for my research during the summer of 2011.

Project Funding 

My project would not have been possible if not for the funding from Hair Massacure, the Stollery Children’s Hospital and the Department of Pediatrics, University of Alberta.