Goal: Empower students to win awards, create products and start companies
        Prof. Phani Kumar Pullela

        PhD, Postdoc, MBA (Pharma)

        E-mail ID: phanikumar.p@cmrit.ac.in
        Mobile No: +91-9902005868/9480573868
      
  
Experience

Seven years of experience in Biomedical device company as Head-Chemistry
Four and half years experience as postdoctoral fellow at Marquette University, USA
Five international awards, More than 10 research grants, more than 12 awards for PKP's students
10 International Patents, 15 research publications, Four products in the market
Experience & enthusiasm in working in any branch of Science or Engineering

Areas of Interest in Teaching

Engineering Chemistry

Supplementary: Any area of chemistry, Grant writing, Idea generation, Product development, Entrepreneurship



Areas of Interest in Research

Chemical Biology, Fluorescence, Biomedical devices, Fluorescent sensors, Product development in any field/branch of Science



Research highlights

  • Bioorganic & Medicinal Chemistry (2003): Selective COX-2 inhibitors (121 citations- considered as a landmark publication in inflammation field
  • Molecular Pharmacology (2002): First Dihydropyridine based T-type calcium channel blocker (PPK-5), this molecule is in clinical trials
  • Tetrahedron Letters (2003): Industrially viable chemical scheme for synthesis of stavudine (process is used worldwide)
  • Molecular Endocrinology (2005): Zebrafish can be a model system for Breast cancer
  • Proteins (2007): First to prove that a second camphor binding site exists in Cytochrome P450cam
  • Journal of Biomolecular NMR (2009): First NMR probe for cytochrome P450s
  • Analytical Biochemistry (2006): Intracellular thiol measurement- A new fluorescence imaging technology- Many products on this concept are in market
  • Advances and Applications in Bioinformatics and Chemistry (2013): First to project that a simple formulation in Vicks inhaler can cure or protect people from H1N1- only research to provide hope for surviving future mutations in influenza virus
  • Renewable Energy (2014): Cashew nut shell liquid based biofuel- first zero cost biofuel in the world
  • Journal of Fluorescence (2014): First to suggest that there need not be spectral overlap for an acceptor to quench the fluorescence of a donor- An attempt to revolutionize the fluorescence field

Grants
  • Department of Defense, USA, Breast Cancer Concept Award (2005-2006)- US$100,000 - First postdoctoral fellow in USA to receive the BCRP concept award
  • Department of Biotechnology (SBIRI scheme, 2007-2008)- Rs 72 Lakhs
  • Defense Research Development Establishment (DRDE, 2008-2009)- Rs 10 lakhs
  • DRDE (2010-2014)-Development of fluorescent gas sensor for nerve agents- Rs  49 lakhs
  • Part of the team which obtained international grants; Grand challenges Canada Can $8,00,000, GCC Phase II- US$3,50,000, FIND Grant: US$ 5,40,000
  • Part of the team which also obtained Indian grants ICMR: Rs 2 Crores, BIPP (DBT): Rs 20 crores, NPMASS: 2 crores, NMITLI Phase II: 4 crores
Research Publications

A novel method for isolation of all major phenolic constituents from cashew (Anacardium occidentale) nut shell liquid.

J. Agric. Food Chem. 49, 2548- 2551, 2001.

Authors: R. Paramshivappa, Phani Kumar Pullela, P.J.Vithayathil, A.Srinivasa Rao

Current citations: 95, Journal Impact Factor: 2.823


Process for isolation of cardanol from technical cashew (Anacardium occidentale) nut shell liquid

J. Agric. Food Chem. 50, 4705-4708, 2002.

Authors: Phani Kumar Pullela, R. Paramshivappa, P.J.Vithayathil, P. V. Subba Rao, A.Srinivasa Rao

Current citations: 84, Journal Impact Factor: 2.823


Synthesis of sildenafil analogues from anacardic acid and their phosphodiesterase-5 (PDE5) Inhibition

J. Agric. Food Chem. 50, 7709-7713, 2002.

Authors: R. Paramashivappa, Phani Kumar Pullela, P. V. Subba Rao, A. Srinivasa Rao.

Current citations: 20, Journal Impact Factor: 2.823


Synthesis and evaluation of a new class of nifedipine analogs with T-type calcium channel activity

Molecular Pharmacology 61, 649-658, 2002.

Authors: Phani Kumar Pullela, Stephanie C Stotz, R. Paramashivappa, Aaron M Beedle, Gerald W. Zamponi,

A. Srinivasa Rao

Current citations: 55, Journal Impact Factor: 6.58

Design, synthesis and biological evaluation of benzimidazole / benzothiazole and benzoxazole derivatives as cyclooxygenase inhibitors

Biorg. Med. Chem. Lett. 13, 657-660, 2003.

Authors: R. Paramashivappa, Phani Kumar Pullela, P. V. Subba Rao, A. Srinivasa Rao.

Current citations: 126, Journal Impact Factor: 2.661


Simple and efficient method for the synthesis of 2', 3'-didehydro-3'-deoxy- thymidine (d4T) Tetrahedron Lett. 44, 1003-1005, 2003.

Authors: R. Paramashivappa, Phani Kumar Pullela, P. V. Subba Rao, A. Srinivasa Rao.

Current citations: 14, Journal Impact Factor: 2.683


Homology modeled ligand-binding domains of zebrafish estrogen receptors #, #1, and #2: from insilico to

invivo studies of estrogen interactions in Danio rerio as a model system

Mol. Endocrinol. 19, 2979- 2990, 2005.

Authors: Aurora D. Costache, Phani Kumar Pullela, Purnachandar Kasha, Daniel S. Sem.

Current citations: 17, Journal Impact Factor: 4.54


Fluorescence based detection of thiols in vitro and in vivo with DSSA probes Anal. Biochem 352, 265-273, 2006.

Authors: Phani Kumar Pullela, Taurai Chiku, Michael J. Caravan III, Daniel S. Sem

Current citations: 65, Journal Impact Factor: 2.996

A dithio coupled kinase and ATPase assay  

J. Biomol. Screen. 11, 844-853, 2006.
Authors: Taurai Chiku, Phani Kumar Pullela, Daniel S. Sem

Current citations: 6, Journal Impact Factor: 2.5


Structural Evidence for a Functionally Relevant Second Camphor Binding Site in P450cam: Model for

Substrate Entry into a P450 Active Site

Proteins: Structure, Function, and Bioinformatics 69, 125- 138, 2007.

Authors: Huili Yao, Christopher R. McCullough, Aurora D. Costache, Phani Kumar Pullela, Daniel S. Sem.

Current citations: 20, Journal Impact Factor: 3.392

13C-Methyl isocyanide as an NMR probe for cytochrome P450 active sites

Journal of Biomolecular NMR 43, 171-178, 2009.

Authors: Christopher R. McCullough, Sang-Choul Im, Lucy Waskell, Phani Kumar Pullela, and Daniel S. Sem.

Currently cited: 3, Journal Impact Factor: 3.047

Design of multiligand inhibitors for the swine flu H1N1 neuraminidase binding site

Advances and Applications in Bioinformatics and Chemistry 6, 47-53, 2013
Authors: Manoj M Narayanan, Chandrasekhar B Nair, Shilpa K Sanjeeva, PV Subba Rao, Phani K Pullela, Colin J Barrow


Selected Patents

1,4- dihydropyridine and pyridine compounds as calcium channel blockers

United States Patent 6,852,742

Inventors: Phani Kumar Pullela, R. Paramashivappa, A Srinivasa Rao, P.V. Subbarao, Jeffrey A. Bibbs

This project was coined to develop a dihydropyridine based T-type calcium channel blocker and the product

PPK-5 was licensed to Diakron Pharmaceuticals, San Diego and it is under clinical trials.

 

Dithio Compounds

United States Patent 7,820,833

Inventors: Phani Kumar Pullela, Daniel S Sem

These molecules are the first FRET based probes for intracellular thiol quantitation and the molecules were licensed to a company in Milwaukee, USA and the work was published in "Analytical Biochemistry".

 

A biofuel composition, process of preparation and a method of fueling thereof

PCT/IN08/00413, WO/2009/004652

Inventors: Chandrasekhar B Nair, Phani Kumar Pullela, GM Kini, PV Subbarao

This patent describes a novel biofuel, which is different from the first generation biofuels. Production of this

biofuel increases food rather depleting it and it never requires transesterification.

 

A biofuel composition, process of preparation and a method of fueling thereof

PCT/IN2009/000445

Inventors: Chandrasekhar B Nair, Phani Kumar Pullela, GM Kini, PV Subbarao
This fuel additive converts any triglyceride to a drop-in diesel. It is suitable for plant, animal and algal
triglycerides and essentially eliminates the transesterification process from biofuel preparation.

                                   

Amplified fluorescence polymers and sensor thereof

PCT/IN2009/007260

Inventors: Anil Kumar, Jasmine Sinha, Phani Kumar Pullela.

It is a 'clickable' amplifying fluorescent polymer which can be tweaked to any antigen-antibody based

reaction and improve its sensitivity by many fold.

 

A method for isolation of nucleic acids and a kit thereof

WO/2011/083429

Inventors: Phani Kumar Pullela, Manoj MN, Santhosh Kumar G, Pinto MP, Chandrasekhar Nair B, Subbarao PV

It relates to isolation and purification of nucleic acids using a proprietary matrix.


Student awards for CMRIT students under Prof. Phani's guidance

          

Students

Competition

Place/ position

Syed Abrar Hussaini, Raazia Fathima, Rushingwa Grace

IEEE Aleyhum 2014

One of the 22 winners, Asia level competition

Juhi joseph & Sai Apoorva

Ideas for India

1st Place, national level

Kavya K & Priyanka M

Ideas for India

3rd place, national level

Mrunalini Srinivas & Aishwarya Jakka

Ideas  for India

Consolation prize, national level

Pavan kondoji, Sruthi R, Rinita KM, & Pawani Tungana

CMR IMS Eureka 2K15

2nd Prize, Bangalore level

Kondoji Pavan

Ideathon 2.0

Two ideas in top 100, Asia level competition

 Prabesh Khadka

Ideathon 2.0

Two ideas in top 100, Asia level competition

Mrunalini Srinivas, Aishwarya Jakka, Nikita Sara Sunil, Ashwini Rajasekhar Bhat

FKCCI

Final 5, consolation prize, Karnataka level

Mrunalini srinivas, Aishwarya Jakka, Nikita Sara Sunil, Ashwini Rajasekhar Bhat

TP Pitch Off

Final 11, National level

Tarun B

Srishti, a state level engineering student competition

First place, Idea Impact, Swaccha Bharat category, State level

Kavya K, Chandni Singh, Aishwarya Shrivastava, Rachana

Srishti, a state level engineering student competition

First Place, ICT for Rural development category, State level

Samata R, Priyanka Purva, Sneha Mariam John, Lavanya N

Srishti, a state level engineering student competition

First Place, POC in Health Care category, State level

Mechanical engg. final year students

KSCST

One of the 400 project awardees, State level