"Research on the development of biosensors for the determination of nickel ions from food raw materials and food"
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About Project
About Project
Title: "Research on the development of biosensors for the determination of nickel ions from food raw materials and food"
Acronim: SenzNi
Contract no. PD 17 din 05/08/2020
P1 - Dezvoltarea sistemului național de CD
Subprogramul 1.1 – Resurse Umane, Proiecte de Cercetare Postdoctorală
PLANUL NAŢIONAL DE CERCETARE, DEZVOLTARE ŞI INOVARE 2015-2020, PNIII
Project duration: 24 months
Project value: 246.950,00 LEI
RESEARCH TEAM
Lecturer dr. eng. Liliana ANCHIDIN-NOROCEL - Project Manager
Prof. dr. eng. Sonia AMARIEI - Mentor
Brief presentation of the results obtained
The research from this postdoctoral project led to the obtaining of biosensors for the determination of nickel ions in food. The importance of determining the concentration of nickel ions in food raw materials and foods is represented by their toxicity on the health of the population, and especially by the occurrence of allergies. Several versions of biosensors have been developed to reach an optimized version, also presented in the patent application that claims the concentration of the biologically active receptor and the immobilization agent, aspects that are the basis of the device's operation. The results of the project were realized by publishing 7 articles, participating in 6 international conferences, and sending a patent application to OSIM. Through these disseminated results, electrode manufacturers as well as those who deal with electronics will have access to the developed methods, so that they can make these biosensors available to people with hypersensitivity to nickel ions to consume food at the level of preventing the occurrence of allergies.
Prezentarea succinta a rezultatelor obtinute
Cercetarile din acest proiect postdoctoral au dus la obtinerea unor biosenzori pentru determinarea ionilor de nichel din alimente. Importanța determinării concentrației ionilor de nichel în materiile prime alimentare și alimente este reprezentată de toxicitatea acestora asupra sănătății populației, si in special de aparitia alergiilor. S-au dezvoltat mai multe variante de biosenzori pentru a se ajunge la o varianta optimizata, prezentata si in cererea de brevet care revendica concentratia receptorului biologic activ si a agentului de imobilizare, aspecte care stau la baza functionarii dispozitivului. Rezultatele obtinute ale proiectului s-au concretizat prin publicarea a 7 articole, participarea la 6 conferinte internationale si trimiterea unei cereri de brevet catre OSIM. Prin aceste rezultate diseminate, producătorii de electrozi dar şi cei care se ocupă de electronică vor avea acces la metodele dezvoltate, astfel încât să poată pune aceşti biosenzori la dispoziţia persoanelor cu hipersensibilitate la ionii de nichel pentru a consuma alimente la nivelul de prevenire a apariţiei alergiilor.
Abstract
Abstract
The research topic addressed in this project was the development of (bio)sensors for the quantification of nickel ions in food raw materials and foods. Nickel ions cause allergies and are found in foods such as: cocoa, chocolate, marzipan, beans, buckwheat, nuts, almonds, peanuts, spinach, wheat, brown rice, sunflower seeds, soybeans, cabbage, pineapple, raspberries, crustaceans, salmon, etc. It seems that about 15% of the human population suffers from nickel allergy. In addition to digestive manifestations, food intolerance to nickel may also have systemic manifestations, such as: diffuse dermatitis, diffuse itching, fever, rhinitis, headache, altered general condition. Increased toxicity can lead to blockage of liver function with the installation of liver coma. Nowadays, most existing methods used for heavy metals trace analysis include spectrophotometric (AAS, ICP/MS, ICP/AES), voltammetric and chromatographic methods, which can detect analytes at very low concentrations. However, all these classic techniques are generally costly and laborious, so it is necessary to develop new methods that eliminate these disadvantages. Therefore, developing a fast, not expensive and in situ method of determining nickel ions in the case of allergies is extremely important, as it could prevent them and would be very useful for the health of the human population. Once developed with the aid of this project and tested on real samples of food raw materials and foods, this (bio)sensor was characterized in terms of analytical performance characteristics. The results was published either in a journal indexed in International Databases and submitted as a patent proposal to OSIM, as well as presented to international conferences.
Project objectives:
Project objectives:
- The development of a (bio)sensor for the determination of nickel ions in food raw materials and foods, by establishing the type of (bio)sensor, identifying the specific biological receptor for analyte of interest and selecting the method of immobilization;
- Testing of the (bio)sensor prototype for the quantitative analysis of nickel in food raw materials and foods;
- Comparison of the results obtained from the tested (bio)sensors with the results obtained by a reference method;
- Advanced (bio)sensors characterization by analysis of analytical performance parameters and analysis of the electrode surface in the (bio)sensor system, after its use on food raw materials and foods samples.
Estimated results of the project
Estimated results of the project
The expected results for achieving the objectives, after optimizing the concentrations of the biological receptors and the immobilization solution, include:
4 - publication of scientific articles;
2 - participation at international conferences;
1- submission of a patent proposal to OSIM;
1 -review article
The obtained results of the project
The obtained results of the project
The obtained results of the project are:
6 - published articles;
6 - participation at international conferences;
1 - submission of a patent proposal to OSIM;
1 - review article
Dissemination of results
Dissemination of results
The results of the project were disseminated by participating in various international conferences, publishing articles and filing a patent.
Conferences:
Participation at the international conference: 8th International Symposium on Sensor Science (ISI conference), 17–28 May 2021, online with paper “SENSING OF NICKEL(II) IONS BY IMMOBILIZING LIGANDS AND USING DIFFERENT SPEs”, authors: Liliana Norocel, Sonia Amariei, Gheorghe Gutt. The link to access the poster is: https://www.mdpi.com/article/10.3390/I3S2021Dresden-10106/s1. Also the abstracts were published in the special volume of the journal Engineering Proceedings (ISSN 2673-4591) and can be accessed at the following link: https://www.mdpi.com/2673-4591/6/1/2.
Participation at the international conference: XXIth International Multidisciplinary Scientific GeoConference Surveying, Geology and Mining, Ecology and Management – SGEM 2021, 14 - 22 August, 2021, Albena, Bulgaria, with paper „IN SITU ELECTROCHEMICAL METHOD FOR DETERMINING NICKEL IONS IN FRUITS”, authors: Liliana Anchidin-Norocel, Gheorghe Gutt, Sonia Amariei. The paper presented was of a poster type, and the conference program can be accessed at the following link: https://www.sgem.org/documents/2021/programme/_Day6_programme_poster.pdf
Participation at the international conference: 8th Edition of the International Conference BIOTECHNOLOGIES, PRESENT AND PERSPECTIVES, Stefan cel Mare University of Suceava, Romania, November 5, 2021, with paper „Voltammetric determination of traces of nickel(II) from foods”, authors Liliana Anchidin-Norocel. The conference program can be accessed at the link: https://fiajournal.usv.ro/conference2021/doc/CONFERENCE%20Program_5%20noiembrie%202021.pdf and the abstracts were published in a special volume with ISSN 2068 – 0819, which can be accessed at: https://fiajournal.usv.ro/conference2021/doc/Abstract%20Conference%20BOOK_V2%20November%202021.pdf
Participation at the international conference: 6th ASIA PACIFIC International Modern Sciences Congress, December 15-16, 2021, online, organized in Delhi, India Mata Sundri College for Women, University Of Delhi, oral presentation of the paper „THE ANALYTICAL PERFORMANCE CHARACTERISTICS FOR NI(II) VOLTAMMETRIC SENSORS DEPENDING ON IMMOBILIZATION ELEMENTS AND TYPES OF SCREEN-PRINTED ELECTRODES”, authors: Liliana Anchidin-Norocel, Sonia Amariei. Abstract book can be accessed at: https://www.iksadasia.org/_files/ugd/614b1f_a3d4715421b04954870a6a383bbc9c33.pdf and the conference program can be accessed at: https://en.iksadasia.org/_files/ugd/614b1f_18940de134bf4e34aa225841b9bc669c. pdf.
Participation at the 3 International Conference on Raw Materials to Processed Foods, 18-19 MAY 2023, ISTANBUL,TURKEY, poster presentation, "A simple electrochemical method for nickel detection in vegetables and fruits", authors: Liliana Anchidin-Norocel, Sonia Amariei, the conference program can be accessed at:
https://www.rpfoods.net/_files/ugd/759bc3_9d7abe2a2a5443939ead985ea9569d23.pdf
Abstract submission for participation at XXIII rd SGEM GeoConference – “Green Science for Green Life” Sessions, Viena 28.11-02.12.2023, poster presentation "VOLTAMMETRIC BEHAVIOR AND DETERMINATION OF NICKEL IONS USING BIOPOLYMERS FOR RECEPTOR IMMOBILIZATION", Liliana Anchidin-Norocel, Gheorghe Gutt, Sonia Amariei.
Published articles:
Liliana Anchidin-Norocel, Wesley K. Savage, Gheorghe Gutt, Sonia Amariei, Development, optimization, characterization and application of electrochemical biosensors for detecting nickel ions in food, Biosensors, 2021, Impact Factor: 5.519, ISSN: 2079-6374. https://www.mdpi.com/2079-6374/11/12/519
Anchidin-Norocel, L., Amariei, S., & Gutt, G. (2021). Determination Of Trace Levels Of Nickel (Ii) By Cyclic Voltammetry With SPEs From Food Products. Food and Environment Safety Journal, 20(3). http://fens.usv.ro/index.php/FENS/article/view/824
Anchidin-Norocel, L., Gutt, G., & Amariei, S. (2021). IN SITU ELECTROCHEMICAL METHOD FOR DETERMINING NICKEL IONS IN FRUITS. International Multidisciplinary Scientific GeoConference: SGEM, 21(6.1), 101-106. 10.5593/sgem2021/6.1/s25.22 https://www.sgem.org/index.php/component/jresearch/?view=publication&task=show&id=8266
Anchidin-Norocel, Liliana, Wesley K. Savage, Roxana Gheorghita, and Sonia Amariei. 2023. Biopolymers Used for Receptor Immobilization for Nickel-Detection Biosensors in Food, Micromachines 14, no. 8: 1529. Impact Factor: 3.4, https://doi.org/10.3390/mi14081529
Gheorghita, R., Filip, R., Lupaescu, A. V., Iavorschi, M., Anchidin-Norocel, L., & Gutt, G. (2023). Innovative Materials with Possible Applications in the Wound Dressings Field: Alginate-Based Films with Moringa oleifera Extract. Gels, 9(7), 560. Factor de impact 4.6, https://www.mdpi.com/2310-2861/9/7/560
Liliana Anchidin-Norocel, Gheorghe Gutt, Sonia Amariei."VOLTAMMETRIC BEHAVIOR AND DETERMINATION OF NICKEL IONS USING BIOPOLYMERS FOR RECEPTOR IMMOBILIZATION", International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM. https://epslibrary.at/sgem_jresearch_publication_view.php?page=view&editid1=9632
Anchidin-Norocel, L., Gutt, G., Tătăranu, E., & Amariei, S. (2024). Electrochemical Sensors and Biosensors: Effective Tools for Detecting Heavy Metals in Water and Food with Possible Implications for Children's Health. International Journal of Electrochemical Science, 100643. IF=1.5, https://www.sciencedirect.com/science/article/pii/S1452398124001846
Patent Proposal
Patent proposal, with the title “BIOSENZOR PENTRU DETERMINAREA IONILOR DE NICHEL”, no A00738/06.12.2021, RO137529 (A2) 2023-06-30 , authors Liliana Anchidin-Norocel, Gheorghe Gutt, Sonia Amariei.
Project
Project
Activities Stage I
Establishing the type of (bio) sensor, identifying the specific biological receptor for nickel ions and selecting the method of immobilization on screen-printed electrodes;
Testing the prototype (bio) sensor for the quantitative analysis of nickel ions from standard solution;
Activities Stage II
Testing the prototype (bio) sensor for the quantitative analysis of nickel ions in food and food raw materials;
Determination of nickel concentration in food raw materials and food by reference method (AAS);
Comparison of the results obtained by the (bio) sensors tested with the results obtained by a reference method;
Activities Stage III
Advanced characterization of (bio) sensors by analysis of analytical performance parameters and analysis of the electrode surface after use on food samples.
Contact Proiect
Tel: +40 230 520 080
Fax: +40 230 520 080
Email: liliana.norocel@usm.ro
Contact USV
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