The BAC recombineering protocol we used successfully to create several BAC constructs for microinjection into mice. This is based on the method from Copeland et al. that utilizes a temperature inducible recombination system (EL250 bacterial strain).
Sheet for scoring the health of mice after a surgical procedure. This scoring system is referenced highly in guides for developing appropriate animal use protocols. This sheet is simply my layout of the system for easy printing.
Section in situ hybridization (SISH) protocol. This protocol is adapted from the WISH version for use on frozen kidney sections with Dig-labelled riboprobes.
Whole-Mount In Situ Hybridization Protocol. This is the protocol followed for our work on the GUDMAP.org project. It includes using the BioLane machine for the washes.
