Enabling large-scale absolute quantification
An article describing the LRE Analyzer is now available:
Rutledge RG (2011) A Java program for LRE-based real-time qPCR that enables large-scale absolute quantification. PLoS ONE 6(3): e17636 Installation files for the most current version of the LRE Analyzer are available on the LRE qPCR Open Source download page. The LRE Analyzer is a fully featured desktop application that provides the automated analysis and data storage capabilities required by large-scale qPCR projects, wanting to exploit the many advantages of absolute quantification. Foremost is the universal perspective provided by absolute quantification, which among other attributes, provides the ability to directly compare quantitative data derived from diverse sources, such as from different assays, instruments and/or research groups. Furthermore, absolute quantification has important implications for gene expression profiling, in that it provides the foundation for accurately comparing transcript quantities produced by any gene to any other gene, within and between any sample. Based on the application of sigmoidal mathematics in combination with utilizing lambda gDNA as a universal quantitative standard, the LRE Analyzer provides the ability to conduct absolute quantification without construction of standard curves. In addition to enabling large-scale absolute quantification, the LRE Analyzer also expands the fundamental capabilities of real-time qPCR, reflected in part by the quality control capabilities it provides that are not possible using conventional methods. Getting Started The LRE Analyzer has an extensive help set that provides an in-depth introduction to LRE, in addition to guidelines on how to implement LRE quantification. Demonstration database files are provided to help illustrate how the program functions. The Introduction to LRE page provides basic background information about how LRE quantification is conducted, with the LRE Video Overview page providing a three part video describing how LRE was conceived, along with a detailed overview of methods that were used to evaluate the accuracy and dynamic range of LRE quantification. The LRE Literature page provides links to LRE related publications, in addition to two earlier qPCR studies that were instrumental in the development of LRE. Inquires should be sent to: lreqpcr@googlegroups.com. Note that a discussion group for LRE qPCR has also been setup, which you are invited to join:
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