Department of Clinical Sciences

College of Veterinary Medicine & Biomedical Sciences

Animal Cancer Center 142
Colorado State University
300 West Drake Road
Campus Delivery 1620

Fort Collins, CO 80523-1678

Brian.Kalet@colostate.edu

• Department of Chemistry & Biochemistry, University of Colorado at Boulder
• Thesis: Doxorubicin and its Formaldehyde Conjugates
• 4.000 GPA

• Department of Chemistry & Biochemistry, University of Colorado at Boulder
• Thesis: Biological Evaluation of Doxorubicin-formaldehyde Conjugates Targeted to the α_vβ₃ Integrin, Estrogen Receptor and Antiestrogen Binding Site
• 3.673 GPA

• Department of Biochemistry & Molecular Biology, University of Massachusetts at Amherst
• Graduated Magna Cum Laude with Biochemistry and Molecular Biology Departmental Honors
• 3.742 GPA

Postdoctoral Fellow

Biological evaluation of doxorubicin-formaldehyde conjugates:

• Elucidated mechanism of action of doxorubicin-formaldehyde conjugate doxazolidine

Summer Graduate Internship Program:

• Determined role of confidential protein expression on apoB secretion

Ascertained structure/function relationships of T1 domain of human voltage-gated potassium channel clone Kv1.2:

• Performed site-directed mutagenesis of Kv1.2 & cloned mutants into mammalian expression vector
• Transfected CHO and RBL cell lines with aforementioned constructs and determined expression levels by immunohistochemistry

Investigated binding and phagocytosis of β-Amyloid [1-40] on a murine microglial cell line:

• Performed radioligand, fluo-ligand and biotinylated-ligand binding assays using viable cells and cell membranes
• Established phagocytosis assay using carboxylated microspheres

Science Tutor

Maintained Proteins, DNA, HPLC, Chemistry and Microbiology labs and Fermentation Suite

Kalet BT, Anglin SR, Handschy A, O'Donoghue L, Halsey C, Chubb L, Korch C, Duval DL.  Transcription factor Ets1 cooperates with estrogen receptor α  to stimulate estradiol-dependent growth in breast cancer cells and tumors.  Submitted.

Koch TH, Barthel BL, Kalet BT, Rudnicki DL, Post GC, Burkhart DJ.  Anthracycline-Formaldehyde Conjugates And Targeted Prodrugs Thereof.  In Anthracyclines Chemistry and Biology II, Topics in Current Chemistry.  Krohn K, (Ed.), Vol. 283, Springer, Berlin 2008.

Kalet BT, McBryde MB, Espinosa JM, Koch TH.  Doxazolidine Induction of Apoptosis by a Topoisomerase II-independent Mechanism. J Med Chem.  2007 Sep 6; 50 (18): 4493-4500.

Burkhart DJ, Barthel BL, Post GC, Kalet BT, Nafie JW, Shoemaker RJ, Koch TH.  Design, Synthesis, and Preliminary Evaluation of Doxazolidine Carbamates as Prodrugs Activated by Carboxylesterases.  J Med Chem.  2006 Nov 30; 49 (24): 7002-12.

Burkhart DJ, Kalet BT, Coleman MP, Post GC, Koch TH.  Doxorubicin-formaldehyde  conjugates targeting αvβ3 integrin. Mol Cancer Ther.  Featured on the cover, 2004 Dec;3(12):1593-604.

Burke PJ, Kalet BT, Koch TH.  Antiestrogen binding site and estrogen receptor mediate uptake and distribution of 4-hydroxytamoxifen-targeted doxorubicin-formaldehyde conjugate in breast cancer cells. J Med Chem.  2004 Dec 16;47(26):6509-18.

Koch TH, Burkhart DJ, Post GC, Nafie J, Kalet BT, Barthel BL, Rudnicki, DL. Methods of Treating Cancer with Doxazolidine and Prodrugs Thereof.  Docket No. 2848-8PCT.  International Application No. PCT/US06/60367.  2006 Oct.

Koch TH, Coleman MP, Cogan PS, Burke PJ, Post GC, McKenzie A, Jackson K, Kalet BT, Burkhart DJ.  Design, Synthesis and the Use of Targeted Anti-Tumor and Antibiotic Drug-Formaldehyde Conjugates.  CU TTO File No. 2002.120B. 2004 Sep.