TRYPANOSOME GROWTH- PROCYCLIC IN VITRO CULTURE BLOODSTREAM IN VITRO CULTURE RODENT INFECTIONS TRYPANOSOME LINES- Trypanosoma brucei brucei (TREU667) History Trypanosoma brucei brucei (427-221) History Trypanosoma brucei brucei (927) History Trypanosoma brucei brucei (427-800) History Trypanosoma brucei rhodesiense History TRYPANOSOME LYSIS ASSAYS- 1. Short term lysis assay (Hajduk et al., 1989) 2. Survival assay (in vitro) (Pays et al.) 3. In vivo activity (mice) (Barker et al., 2001) 4. Fluorescence killing assay (Raper et al.) TRYPANOSOME LYTIC FACTOR (TLF) PURIFICATION- 1. Flotation/chromatography (Hajduk et al., 1989) 2. Flotation antibody affinity (Shiflett et al., 2005; Widener et al., 2007) 3. Individual apolipoproteins (shiflett et al., 2005) |
protocols |