RUSLAN DMITRIEV | Руслан Игоревич Дмитриев


Curriculum vitae

Department of Biochemistry, University College Cork,

Cavanagh Pharmacy Building, College Road, Cork, Ireland;

Phone: +353(86)1603450 e-mail: r.dmitriev[at]ucc.ie;

Google scholar citations page



 

EDUCATION     

2004–2008, Ph.D. in chemistry (‘bioorganic chemistry’)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. Thesis title: “Structure-functional analysis of the novel protein hampin as a component of the nuclear interactome”.

2002–2004, M.Sc. in Chemistry and technology of biological active substances (distinction)

Moscow State Academy of Fine Chemical Technology, Moscow, Russia. Thesis title: “Gene engineering and immunochemical studies of the Secretory Pathway Calcium ATPase”.

1998–2002, B.Sc. in Chemistry and technology of biological active substances (distinction)

Moscow State Academy of Fine Chemical Technology, Moscow, Russia. Thesis title: “Molecular cloning of 5’-cDNA ends of Secretory Pathway Calcium ATPase isoforms. Production and purification of corresponding polypeptides”.


SOCIETY MEMBERSHIP

Society of general physiologists, Woods Hole, MA (SGP), 2005-2006

American society for cell biology (ASCB), 2011-2012

American society for biochemistry and molecular biology (ASBMB), since 2007             

                                            

AREA OF EXPERTISE

Molecular, chemical and cell biology, biochemistry.

Experience in studies of membrane ion-transporting (Ca-ATPases, X,K-ATPases, H-transporting transhydrogenase) and nuclear proteins such as components of MYST1 histone acetylase complex and RASSF1-interacting proteins. Design and preparation of phosphorescent cell-permeable molecular probes and their application to study metabolic (dys)function. Molecular physiology of effects of low-O2 (hypoxia) on cancer and neuronal-like cell models.

EXPERIMENTAL SKILLS (short list):

Cell culture, subcellular fractionation using centrifugation techniques, immunoenzyme assays, molecular cloning and production of recombinant proteins in E. coli, chromatography, production of polyclonal and monoclonal antibodies, protein-protein interactions, fluorescence microscopy, enzyme assays.

 

WORK EXPERIENCE

2008-present - postdoctoral researcher (contract on SFI project), University College Cork, Cork, Ireland

2004-2008 – minor scientist, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia (full time)

2004-2008 – enzyme analyst, Affiliate laboratory of Newcastle Technical Center of Procter & Gamble, program “Introduction of screening system for chemical and biochemical analysis of detergents” Moscow, Russia (part time)

2001-2004 – student/technician, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia (part time)

 

PATENTS

2009 - Conjugates Of Cell-Penetrating Peptides and Phosphorescent Metalloporphyrins For Intracellular Oxygen Measurement. D Papkovsky, R Dmitriev: WO Patent WO/2010/143,172

PEER-REVIEWED PUBLICATIONS

  1. Pestov NB, Dmitriev RI, Shakhparonov MI: Regulation of plasma membrane Са2+-АТРase. Usp biol khim 2003, 43:99-138.
  2. Dmitriev RI, Pestov NB, Korneenko TV, Sorokina ES, Kazmin AA, Kostina MB, Shakhparonov MI: SPCA, secretory pathway Ca2+-ATP-ase, is predominantly localized in plasma membrane. Biol Membrany 2003, 20:480-485.
  3. Arkblad EL, Tuck S, Pestov NB, Dmitriev RI, Kostina MB, Stenvall J, Tranberg M, Rydstrom J: A Caenorhabditis elegans mutant lacking functional nicotinamide nucleotide transhydrogenase displays increased sensitivity to oxidative stress. Free Radic Biol Med 2005, 38:1518-1525.
  4. Dmitriev RI, Pestov NB, Korneenko TV, Gerasimova AV, Zhao K, Modianov NN, Kostina MB, Shakhparonov MI: [Tissue specificity of alternative splicing products of mouse mRNA encoding new protein hampin homologous to the Drosophila MSL-1 protein]. Bioorg Khim 2005, 31:363-371.
  5. Dmitriev RI, Pestov NB, Korneenko TV, Shakhparonov MI: Intracellular location of hampin isoforms. Dokl Biochem Biophys 2006, 408:130-132.
  6. Dmitriev RI, Korneenko TV, Bessonov AA, Shakhparonov MI, Modyanov NN, Pestov NB: Characterization of hampin/MSL1 as a node in the nuclear interactome. Biochem Biophys Res Commun 2007, 355:1051-1057.
  7. Slizhikova DK, Zinov'eva MV, Kuz'min DV, Snezhkov EB, Shakhparonov MI, Dmitriev RI, Antipova NV, Zavalova LL, Sverdlov ED: [Decrease in expression of human J-chain in lung squamous cell cancer and adenocarcinoma]. Mol Biol (Mosk) 2007, 41:659-665.
  8. Dmitriev RI, Shakhparonov MI, Pestov NB: Structure and function of MYST1 histone acetyltransferase in the interactome of animal cells. Biochemistry (Mosc) 2008, 73:839-852.
  9. Dmitriev RI, Okkelman IA, Abdulin RA, Shakhparonov MI, Pestov NB: Nuclear transport of protein TTC4 depends on the cell cycle. Cell Tissue Res 2009, 336:521-527.
  10. Baklaushev V, Gurina O, Yusubalieva G, Dmitriev R, Makarov A, Chekhonin V: Isolation of Extracellular Recombinant Fragment of Rat Connexin-43. Bulletin of Experimental Biology and Medicine 2009, 148:389-393.
  11. Dmitriev RI, Zhdanov AV, Ponomarev GV, Yashunski DV, Papkovsky DB: Intracellular oxygen-sensitive phosphorescent probes based on cell-penetrating peptides. Anal Biochem 2010, 398:24-33.
  12. Zhdanov AV, Dmitriev RI, Papkovsky DB: Bafilomycin A1 activates respiration of neuronal cells via uncoupling associated with flickering depolarization of mitochondria. Cell Mol Life Sci 2010, 68(5):903-917.
  13. Dmitriev RI, Ropiak HM, Yashunsky DV, Ponomarev GV, Zhdanov AV, Papkovsky DB: Bactenecin 7 peptide fragment as a tool for intracellular delivery of a phosphorescent oxygen sensor. FEBS J 2010, 277(22):4651-4661.
  14. Dmitriev RI, Ropiak HM, Ponomarev GV, Yashunsky DV, Papkovsky DB: Cell-penetrating conjugates of coproporphyrins with oligoarginine peptides: rational design and application for sensing intracellular O(2). Bioconjug Chem 2011, 22:2507-2518.
  15. Pestov NB, Dmitriev RI, Kostina MB, Korneenko TV, Shakhparonov MI, Modyanov NN: Structural Evolution and Tissue-Specific Expression of Tetrapod-Specific Second Isoform of Secretory Pathway Ca2+-ATPase. Biochem Biophys Res Commun 2012, 417:1298-1303.
  16. Dmitriev RI, Papkovsky DB: Optical probes and techniques for O2 measurement in live cells and tissue (review). Cell Mol Life Sci 2012. Epub Jan 17
  17. Dmitriev RI, Zhdanov AV, Jasionek G, Papkovsky DB: Assessment of cellular oxygen gradients with a panel of phosphorescent oxygen-sensitive probes. Anal Chem 2012, 84(6):2930-2938.
  18. Koren K, Dmitriev RI, Borisov SM, Papkovsky DB, Klimant I: Complexes of IrIII-Octaethylporphyrin with Peptides as Probes for Sensing Cellular O2. ChemBioChem 2012. Epub Apr 24

 

BOOKS AND BOOK CHAPTERS

  1. Fercher A, O'Riordan TC, Zhdanov AV, Dmitriev RI, Papkovsky DB: Imaging of cellular oxygen and analysis of metabolic responses of mammalian cells. Methods Mol Biol 2010, 591:257-273. (in: LIVE CELL IMAGING: methods and protocols, Humana Press)

AWARDS

2004 – Special diploma with honors for “active and fruitful research work in successful combination with studies”, Moscow State Academy of Fine Chemical Technology

2004 – Award for the best M.Sc. thesis in “Open competition of students’ research projects”, Moscow State Academy of Fine Chemical Technology

2005 – Travel award for 59th annual meeting of the Society of General Physiologists (‘Na,K-ATPase & Related Cation Pumps: Structures, Mechanisms, & Diseases (September 6-11)’ conference, Woods Hole, MA, USA)

2006 – 2nd prize for oral presentation at “The 1st International Young Scientists Conference: Biology - from molecule to biosphere” (Kharkiv Natonal University, Ukraine)

2007 – ASBMB (American Society for Biochemistry and Molecular Biology) graduate student travel award for Experimental Biology 2007 symposium (Washington DC, USA)

2007 – 2nd prize for oral presentation in “Conference for young scientists, Ph.D. students and students on molecular biology and genetics” (Institute of Molecular Biology & Genetics, Kiev, Ukraine)

2011 - ASBMB postdoctoral travel award for Experimental Biology 2011 symposium (Washington DC, USA)

2011 – ASCB (American Society for Cell Biology) postdoctoral travel award for ASCB 2011 Annual Meeting (Denver CO, USA)

2012 – ASBMB postdoctoral travel award for Experimental Biology 2012 symposium (San Diego CA, USA)

SELECTED CONFERENCES

2005 - ‘Na,K-ATPase & Related Cation Pumps: Structures, Mechanisms, & Diseases’ meeting (6-11 September, Woods Hole, MA, USA) – poster

2006 - The 1st International Young Scientists Conference: Biology - from molecule to biosphere (November, Kharkiv Natonal University, Ukraine) – oral presentation

2009, 2011 – American Society for Cell Biology (ASCB) annual meetings (USA) -  posters

2007, 2011, 2012 – ‘Experimental Biology’ meetings (USA) – oral and poster presentations

PUBLISHED CONFERENCE PROCEEDING

  1. Ruslan I. Dmitriev, Nikolay B. Pestov, Tatyana V. Korneenko, Maria B. Kostina, Mikhail I. Shakhparonov. Characterization of Second Isoform of Secretory Pathway Ca2+/Mn2+-ATPase. J Gen Physiol, 2005, 126, 71a.
  2. Nikolay B. Pestov, Tatyana V. Korneenko, Rossen Radkov, Ruslan I. Dmitriev, Gary Shull, Nikolai N. Modyanov. Nongastric H,K-ATPase in Rodent Anterior Prostate. J Gen Physiol, 2005, 126, 76a.
  3. Ruslan I. Dmitriev, Tatyana V. Korneenko, Mikhail I. Shakhparonov. A Novel Interaction between Transcription Repressor of IL-12 p35 Gene GC BP and Hampin. J Gen Physiol, 2006, 128, 14a.
  4. Ruslan I. Dmitriev, Tatyana V. Korneenko, Nikolay B. Pestov, Nikolay N. Modyanov, Mikhail I Shakhparonov. Identification of novel protein-protein interactions pertinent to regulation of mammalian hampin/MSL1 and histone acetylase MYST1/MOF. FASEB J, 2007, 21, A283-c.
  5. Ruslan Dmitriev, Mikhail I. Shakhparonov, Nikolay B. Pestov. TTC4 provides a functional link between RASSF1C and DNA primase. FASEB J, 2008, 22, 826.1.
  6. Nikolay Pestov, Ruslan Dmitriev, Irina Okkelman, Mikhail Shakhparonov. Hampin/msl1 Regulates Nuclear Transport of Putative Tumor Suppressor TTC4. Mol. Biol. Cell, 2008, 19 (suppl), abstract 2779/B493. (CD-ROM).
  7. R. I. Dmitriev, A. V. Zhdanov, D. B. Papkovsky. New Phosphorescent Oxygen-Sensitive Probes Based on Cell Penetrating Peptides. Mol. Biol. Cell, 2009, 20 (suppl), abstract # 1550/B708.
  8. H.M. Ropiak, R.I. Dmitriev, D.V. Yashunski, G.V. Ponomarev, A.V. Zhdanov, D.B. Papkovsky. Analysis of cellular function by use of cell-penetrating oxygen sensing probes. BBA Bioenergetics, 2010, 1797 (Supplement 1), 129.
  9. Dmitriev RI, Yashunsky DV, Ropiak HM, Zhdanov AV, Ponomarev GV, Papkovsky DB: Rational design and experimental assessment of the new family of intracellular O2-sensitive probes. The FASEB Journal 2011, 25(1_MeetingAbstracts):762.764.
  10. Dmitriev RI, Zhdanov AV, Papkovsky DB: Uncoupling effect of bafilomycin A1 on HIF and cell bioenergetics. The FASEB Journal 2011, 25(1_MeetingAbstracts):861.815.
  11. Dmitriev RI, Fercher A, Zhdanov AV, Papkovsky DB (2011). Oxygen microscopy and high-throughput analysis of icO2 by means of cell-penetrating phosphorescent dyes. Mol. Biol. Cell 22 (suppl), Abstract No. 1670.
  12. Dmitriev RI, Zhdanov AV, Papkovsky DB: Histone H4 acetylation at K16 residue and mitochondrial activity in neuronal cells. The FASEB Journal 2012, 26(1_MeetingAbstracts):565.564.
  13. Dmitriev RI, Zhdanov AV, Jasionek G, Papkovsky DB: Assessment of intracellular O2 gradient on a microplate reader. The FASEB Journal 2012, 26(1_MeetingAbstracts):785.781.


TUTORSHIP

Co-supervision (with PI) of M.Sc. student research projects and 4th year biochemistry projects on a regular basis.

INTERESTS

  • astrobiology and the origin of life
  • film photography (you are welcome to check my Flickr gallery)
  • birdwatching