The Phage Cookbook

Here you find my collection of notes about how to handle phage λ and other phages. I give no guarantee that any of this works - for me it does though. If you spot an error, please tell me.

Table of Contents

Recipes / Handling chemicals

• Media and buffers
• Antibiotics
• Various chemicals
• Enzymes

Various Methods

• DNA isolation from λ lysates using the Wizard^® DNA Clean-Up System (Promega)
• DNA isolation from λ lysates using the Qiagen lambda midi kit
• Confirming single copy λ prophage integration (Powell et al. 1994)
• Recombineering/λ red-mediated gene replacement
• P1 Transduction
• DNA Sequencing
• TSS Transformation
• Flow Cytometry

Handling phages

• Preparing a phage stock
• How to store bacteria and phage
• Quantification of phages - on a plate
• Quantification of phages - real time PCR with SYBR Green
• How to make a proper dilution series
• Using λ JL259, which makes blue colonies

Collections

• List of all strains in the collection
• List of all PCR primers

Other things

• References
• Expressing yourself properly