 José A. M. da Costa Nunes, DPhil
Contact details:
Keywords:
Arabidopsis
DNA double strand break (dsb) damage repair Meiosis cohesins
chromosome segregation
genome integrity
Research area: I am currently interested in the biological mechanisms involved in
DNA double strand break (dsb) repair in Arabidopsis, as well
as in chromosome segregation. DNA dsb can be caused by abiotic
factors (chemicals, ionising radiation - gamma rays, UV rays, X-rays)
but also occur during meiosis before chromosome segregation takes place.
To characterise these mechanisms in plants further I am using EMS
meiotic mutants and reverse genetics as a tool to study the roles DNA
repair and chromosome cohesion. Microscopy analysis and biochemical
characterisation are also being implemented.
DAPI stained Arabidopsis thaliana male meiocytes; meiosis II mutant phenotypes. 2008 - ... - Research project (funded by the Portuguese Research Funding Agency - FCT)
PTDC/BIA-BCM/64192/2006 - "Chromosome Cohesion and DNA repair in Arabidopsis"
PI of the Project - José A. M. da Costa Nunes
 Education:
1996 Traineeship at I.G.E.R. (Institute for Grassland and Environmental Research) (Aberystwyth, Wales , U.K. )
1997-2001 DPhil (PhD) degree,
Department of Plant Sciences, University of Oxford, U.K.
Supervisor: Professor Hugh Dickinson
Seedlings of w.t. (top) and radiation sensitive Arabidopsis mutant (bottom)
Post-Doc Research:
2001 Short-term Post-doc,
Department of Plant Sciences, University of Oxford, U.K.
Professor Hugh Dickinson's laboratory
2002-2005 Pflanzenbiologie Institut, Zürich Universität, Switzerland Professor Ueli Grossniklaus' laboratory
2005-2008 Universidade Técnica de Lisboa, Portugal
2008- ... Universidade Nova de Lisboa, Portugal (with own funded Research Project (PI) and Fellowship)
Affiliations/Memberships:
1998 - 2000; 2009/2010- to the present - The Society of Experimental Biology (S.E.B.), ( U.K. )
1997 - to the present - Genetics Society ( U.K. )
1997 - 2001 - Wolfson College, University of Oxford , U.K.
Peer reviewed Publications: Research Article(s)
da Costa-Nunes, J.A. and Viegas, W. (2009) An approach to screen and identify novel meiotic mutants in an (Arabidopsis) EMS mutant population. In Shu QY (ed) Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations, Rome, 2009, pages 126-128
http://www.fao.org/docrep/012/i0956e/i0956e04.pdf (Cited in _ articles: source - ISI Web of Science)
Kozak J., West C.E., White, C., da Costa-Nunes, J.A., Angelis K.J. (2009) Rapid repair of DNA double strand breaks in Arabidopsis thaliana is
dependent on proteins involved in chromosome structure maintenance. DNA Repair 8: 413-419
http://dx.doi.org/10.1016/j.dnarep.2008.11.012
Journal's Impact factor: 4.293 (Cited in _ articles: source - ISI Web of Science) da Costa-Nunes J.A., Bhatt A.M., O'Shea S., West C.E., Bray C.M., Grossniklaus U., Dickinson H.G. (2006). Characterization of the three Arabidopsis thaliana RAD21 cohesins reveals differential responses to ionizing radiation. Journal of Experimental Botany 57: 971-983
http://dx.doi.org/10.1093/jxb/erj083
Journal's Impact factor: 4.818 (Cited in _ articles: source - ISI Web of Science)
Page D.R., Köhler C., da Costa-Nunes J.A.; Baroux C., Moore J.M., Grossniklaus U. (2004). Intrachromosomal excision of a hybrid Ds element induces
large genomic deletions in Arabidopsis. Proc. Natl. Acad. Sci. USA. 101: 2969-2974
Journal's Impact factor: 9.771 (Cited in _ articles: source - ISI Web of Science)
Peer reviewed Publications: Review Article(s)
da Costa-Nunes J.A., Grossniklaus U. (2003). Unveiling the gene-expression profile of pollen. Genome Biology 5: 205.1-205.3.
Journal's Impact factor: 6.89 (Cited in _articles: source - ISI Web of Science)
Contribution to oral communications in International Conferences:
A.M. Bhatt, J. Costa-Nunes, Y. Li & H.G. Dickinson (2001) "Functional analysis of Arabidopsis cohesins" Journal of Experimental Botany 52
(March Supplement): 102 (5th European Meiosis Meeting - 2nd - 6th April)
A.M. Bhatt, J. Costa-Nunes, Y. Li, F. Osman, H.G. Dickinson, C. Lister, C. Dean, G. Jones, P. Fransz (1999) "Functional analysis of Arabidopsis cohesins - DIF1 and AtRAD21" (4th European Meiosis Meeting, 18th - 22nd September 1999)
A.M. Bhatt, J. Costa-Nunes, F. Osman, C. Lister, P. Fransz, G.J.H. Jones, J. Doonan, C. Dean & H.G. Dickinson (1999) "Meiotic roles of the Arabidopsis cohesin DIF1" (XVI International Botanical Congress, St. Louis, Missouri, U.S.A.)
Meeting abstracts and posters in International and UK Conferences: J. A. da Costa Nunes; R. B. Ferreira & W. Viegas (2009) “Early microspore development stage histological markers for sterile and semi-sterile mutant
Arabidopsis screens”, - 9th IPMB Congress - Conference Program. page 109
(IPMB - St. Louis, MO, USA, 25th - 30th October 2009)
J. A. da Costa Nunes & W. Viegas (2008) “An approach to screen and identify novel meiotic mutants in an EMS mutant population” IAEA-CN-167-321P
- Food and Agriculture Organisation/International Atomic Energy Agency - International Symposium on Induced Mutations in Plants. – Book of Abstracts. page 36
(ISIMP – Vienna, Austria, 12th - 15th August 2008)
J.A. da Costa-Nunes; A.M. Bhatt, S.O’Shea, C.E. West, C.M. Bray, U. Grossniklaus & H.G. Dickinson (2006) “The AtRAD21 genes and their response
to DNA damaging ionising radiation” POS-THU-590 - ISPMB, 8th International Congress of Plant Molecular Biology - Book of Abstracts. page 224
(ISPMB - Adelaide, Australia, 20th - 26th August 2006)
D.R. Page, C. Köhler, J. Costa-Nunes , C. Baroux, J.M. Moore, & U. Grossniklaus (2003) " Excision of a hybrid Ds element induces large genomic deletions
in Arabidopsis” (Plant Genetics 2003; October 22-26, 2003; Snowbird Resort & Conference Center, Snowbird, Utah)
A.M. Bhatt, J. Costa-Nunes , A. Lagodienko & H.G. Dickinson (2002) "Functional analysis of cohesins during meiosis" Comparative Biochemistry and
Physiology Part A 132 : S132-S133 (Society for Experimental Biology Annual Meeting, Swansea , U.K. , 8 th - 12 th April 2002)
A.M. Bhatt, J. Costa-Nunes , Y. Li & H.G. Dickinson (2001) “Functional analysis of Arabidopsis cohesins”
(Chromosome Dynamics & Expression, the Fourteenth John Innes Symposium, 5 th - 8 th September 2001)
A.M. Bhatt, J. Costa-Nunes , Y. Li & H.G. Dickinson (2001) "Functional analysis of Arabidopsis cohesins" Journal of Experimental Botany 52 (March
Supplement): 102 (5th European Meiosis Meeting, 2 nd - 6 th April 2001)
A.M. Bhatt, J. Costa-Nunes , F. Osman, S. O'Shea, Y. Li & H.G. Dickinson (1999). " DIF1 is a homologue of the S. pombe REC8 / RAD21 cohesins, and
is essential for meiosis in Arabidopsis thaliana "
(Genetics of Growth & Cell Division, The Genetical Society Spring Meeting - 24 th - 26 th March 1999)
J. Costa-Nunes , A. M. Bhatt, M. Spielman, C. Canales, R. Scott & H.G. Dickinson. (1998) "Genes Essential for Meiosis in Arabidopsis"
(Meiosis Gordon Conference, Colby-Sawyer College, New Hampshire, U.S.A. - 14 th - 19 th June 1998)
J. Costa-Nunes , A.M. Bhatt, S.F. O'Shea & H.G. Dickinson (1998) " DIF1 , a gene essential for meiosis, and its homologue." Journal of Experimental
Biology 49 (May Supplement): 85 (Society for Experimental Biology Annual Meeting, University of York, York , U.K., 23 rd - 27 th March 1998)
A.M. Bhatt, J. C. Nunes , S. O'Shea, F. Osman, S. McCready & H.G. Dickinson (1998) "Functional analysis of DIF1 , an Arabidopsis gene essential for
meiosis." Journal of Experimental Biology 49 (May Supplement): 81
(Society for Experimental Biology Annual Meeting, University of York, York, U.K., 23 rd - 27 th March 1998).
- Costa Nunes, J.A., Bhatt, A.M. and Dickinson, H.G. (2001); Database Direct Sequence Submission AF400129 Arabidopsis thaliana RAD21-3 mRNA,
complete cds gi|18157648|gb|AF400129.1|AF400129[18157648]
- Costa Nunes, J.A., Bhatt, A.M. and Dickinson, H.G. (2001); Database Direct Sequence Submission AF400128 Arabidopsis thaliana RAD21-2 mRNA, complete cds gi|18157646|gb|AF400128.1|AF400128[18157646]
- Costa Nunes, J.A., Bhatt, A.M. and Dickinson, H.G. (2001); Database Direct Sequence Submission AF400127 Arabidopsis thaliana RAD21-1 variant 2 mRNA,
complete cds gi|18157644|gb|AF400127.1|AF400127[18157644]
- Costa Nunes, J.A., Bhatt, A.M. and Dickinson, H.G. (2001); Database Direct Sequence Submission AF400126 Arabidopsis thaliana RAD21-1 variant 1 mRNA,
complete cds gi|18157642|gb|AF400126.1|AF400126[18157642]
Last updated on
|
