The laboratory's research program focuses on the molecular processes involved in signal transduction across biological membranes. In particular we study the mechanisms controlling the efficacy of G protein-coupled receptor (GPCR) signaling pathways. For instance, several projects aim at describing, in molecular terms, the link between ligand binding and signaling efficacy. We also study regulatory processes such as receptor phosphorylation, palmitoylation and endocytosis that control hormonal responsiveness. In recent years, we and others have suggested that receptor dimerization and oligomeric assembly play important roles in receptor biogenesis and signaling. Using biophysical approaches such as bioluminescence resonance energy transfer (BRET), we continue to investigate the nature and roles of protein-protein interactions in signal transduction. Our work on GPCR biogenesis revealed that compounds known as pharmacological chaperones can facilitate the proper maturation and cell trafficking of ER-retained mutant receptors. The precise mechanism of action of pharmacological chaperones as well as their potential use in the treatment of conformational diseases are also under investigation. Please click on the links to learn more about each of these research themes.