JAMSHID RAHEB 9041 YONGE ST, RICHMOND HILL, ON, CANADA, L4C 0L2 jamshid_raheb@yahoo.com jam@nigeb.ac.ir (905)886-2338 (416) 587-3032 : Enclosed please find my resume; I graduated from the University of Warwick from United Kingdom in May 1998 with a PhD Degree in molecular microbiology. My major was Genetic Engineering. As you will see from my resume, in addition to meeting the demands of a full-time academic schedule, I have also accumulated fifteen years of experience in biotechnology research. Sincerely, Jamshid Raheb Jamshid Raheb PersonalDate and Place of Birth: March 17-1962 – Tehran – IRAN Marital Status: Single ObjectiveSearching, learning and teaching Education Research work Ph. D in Molecular Microbiology 1994-1998 The University of Warwick ,U.K. ENGLAND -Thesis: The molecular and physiological effects of starvation and other stresses on Flexibacter chinensis Average grade: A+ For many years physiological, morphological and particularly molecular studies on gliding bacteria were impossible due to the lack of a suitable technique for the genetic manipulation of this group of bacteria including Flexibacter chinensis. Our study has focused on the physiological and morphological changes in molecular level in this organism during the transition from the exponential to stationary phase of growth, and during long -term stress condition. Our data demonstrate that there is a dramatic change in respiration activity and enzymes during the stationary phase which can not be seen in the exponential phase. Parallel to this bacterial morphology changes with a particularly noticeable size reduction in this usually filamentous bacterium. With the collaboration of Wisconsin University in USA we have developed a method for the molecular study of this group of bacteria. We have constructed two mutants for the genes which produce two novel sigma factors, KatF(sS) and RpoH(s32), and have investigated the effect of these mutants with respect to cell size changes during the transition from growth to starvation conditions and under heat stress. The mutants were unable to change from the filamentous to coccoidal form and consequently were unable to survive stress as effectively as the parental strain which could undergo morphological changes. Our data have demonstrated the importance of cell division in Flexibacter chinensis for the survival of this bacterium under stress conditions. Master of Science (M.Sc) in Bacteriology 1987-1991, Tarbiat Modarres University, Tehran, IRAN Average grade: 17.62 / 20 -Thesis: Investigation of fastidious E. coli in the urinary tract infections Thesis Grade: 19.50 / 20 Bachelor of Science in Biology (B.Sc) in Microbiology Aug. 1983 – Jul. 1987, Tehran University , Tehran, IRAN Average grade: 17.60 / 20 The Microbial sulfur-specific transformation which selectively desulfurize organic sulfur compounds in fossil fuels, has been identified. We have Identified, Isolated, cloned and sequenced a 4kb gene locus from a newly isolated Iranian strain Rhodococcus FMF(4S pathway genes) on a broad host range plasmid with integration ability in chromosome of gram negative bacteria. This was reported for the first time as chromosomal conserved dsz genes from 4S pathway in Rhodococcus which these genes recorded in EMBL(1,2,3,4). This organized as one operon with three genes (dszA, dszB and dszC)under the control of single promoter. The dsz gene from this new strain has been cloned under control of tac promoter in different pseudomonas solvent tolerant species(ATCC 9027). We have applied an industrial microorganism , with the ability in biological desulfurization. In this way, using molecular and microbiological techniques we successfully constructed a manipulated bacterium with two relevant industrial application, that is biosurfactant production and desulfurization of fossil fuel. I have selected a strain of pseudomonas. Sp. which is producing biosurfactants which can help the desulfurization process by accelerating phase separation. In this regard I have cloned Rhodococcus Erytheropolis(IGTS8) desulfurization operon with a heterologues promoter(tac) in this pseudomonas strain. The resulted construct has three industrial traits. First, it has an enhanced desulfurization activity due to the exchange of native promoter with heterologues tac promoter. Second, it can produce biosurfactants which in turn help to desulfurization process by enhancing phase separation. Third, as pseudomonas Sp. is the most known solvent tolerance bacteria, this construct has the advantage to be used in industrial operation. In further step I am going to conduct a research on likely interactions between Rhamnolipid biosurfactants and oil components. Further researches is in progress to find out the influence of the biosurfactants on DBT desulfurization. By the way I am to appreciate the close cooperation of Dr Di

JAMSHID RAHEB 9041 YONGE ST, RICHMOND HILL, ON, CANADA, L4C 0L2 jamshid_raheb@yahoo.com jam@nigeb.ac.ir (905)886-2338 (416) 587-3032 : Enclosed please find my resume; I graduated from the University of Warwick from United Kingdom in May 1998 with a PhD Degree in molecular microbiology. My major was Genetic Engineering. As you will see from my resume, in addition to meeting the demands of a full-time academic schedule, I have also accumulated fifteen years of experience in biotechnology research. This experience gave me the opportunity to work closely with a team of biochemists and bioengineers. Sincerely, Jamshid Raheb Jamshid Raheb PersonalDate and Place of Birth: March 17-1962 – Tehran – IRAN Marital Status: Single ObjectiveSearching, learning and teaching Education Research work Ph. D in Molecular Microbiology 1994-1998 The University of Warwick ,U.K. ENGLAND -Thesis: The molecular and physiological effects of starvation and other stresses on Flexibacter chinensis Average grade: A+ For many years physiological, morphological and particularly molecular studies on gliding bacteria were impossible due to the lack of a suitable technique for the genetic manipulation of this group of bacteria including Flexibacter chinensis. Our study has focused on the physiological and morphological changes in molecular level in this organism during the transition from the exponential to stationary phase of growth, and during long -term stress condition. Our data demonstrate that there is a dramatic change in respiration activity and enzymes during the stationary phase which can not be seen in the exponential phase. Parallel to this bacterial morphology changes with a particularly noticeable size reduction in this usually filamentous bacterium. With the collaboration of Wisconsin University in USA we have developed a method for the molecular study of this group of bacteria. We have constructed two mutants for the genes which produce two novel sigma factors, KatF(sS) and RpoH(s32), and have investigated the effect of these mutants with respect to cell size changes during the transition from growth to starvation conditions and under heat stress. The mutants were unable to change from the filamentous to coccoidal form and consequently were unable to survive stress as effectively as the parental strain which could undergo morphological changes. Our data have demonstrated the importance of cell division in Flexibacter chinensis for the survival of this bacterium under stress conditions. Master of Science (M.Sc) in Bacteriology 1987-1991, Tarbiat Modarres University, Tehran, IRAN Average grade: 17.62 / 20 -Thesis: Investigation of fastidious E. coli in the urinary tract infections Thesis Grade: 19.50 / 20 Bachelor of Science in Biology (B.Sc) in Microbiology Aug. 1983 – Jul. 1987, Tehran University , Tehran, IRAN Average grade: 17.60 / 20 The Microbial sulfur-specific transformation which selectively desulfurize organic sulfur compounds in fossil fuels, has been identified. We have Identified, Isolated, cloned and sequenced a 4kb gene locus from a newly isolated Iranian strain Rhodococcus FMF(4S pathway genes) on a broad host range plasmid with integration ability in chromosome of gram negative bacteria. This was reported for the first time as chromosomal conserved dsz genes from 4S pathway in Rhodococcus which these genes recorded in EMBL(1,2,3,4). This organized as one operon with three genes (dszA, dszB and dszC)under the control of single promoter. The dsz gene from this new strain has been cloned under control of tac promoter in different pseudomonas solvent tolerant species(ATCC 9027). We have applied an industrial microorganism , with the ability in biological desulfurization. In this way, using molecular and microbiological techniques we successfully constructed a manipulated bacterium with two relevant industrial application, that is biosurfactant production and desulfurization of fossil fuel. I have selected a strain of pseudomonas. Sp. which is producing biosurfactants which can help the desulfurization process by accelerating phase separation. In this regard I have cloned Rhodococcus Erytheropolis(IGTS8) desulfurization operon with a heterologues promoter(tac) in this pseudomonas strain. The resulted construct has three industrial traits. First, it has an enhanced desulfurization activity due to the exchange of native promoter with heterologues tac promoter. Second, it can produce biosurfactants which in turn help to desulfurization process by enhancing phase separation. Third, as pseudomonas Sp. is the most known solvent tolerance bacteria, this construct has the advantage to be used in industrial operation. In further step I am going to conduct a research on likely interactions between Rhamnolipid biosurfactants and oil components. Further researches is in progress to find out the influence of the