- Design and construction of flow loop model for oilfield tubulars and vessels and design and construction of sand pack reservoir model.
- The first experiments will be aimed at developing, testing and verification of methodology to inoculate, colonize and measure activity of bacterial strains provided by the Institute of Phage Technology. These strains will be models of microbes important in:
- MIC, inoculated into a flow loop designed to model oilfield tubulars and vessels
- Reservoir souring, inoculated into a sand pack reservoir model
- The next step will be development, testing and verification of methodology to test efficacy of biocides (and later phage) to control microbial colonies in the laboratory models (a.) and (b.) above. A critical part of this step will be developing reliable methodology to determine where and under what conditions in the models microbial colonies can resist control by biocides.
- Once reliable standardized methodologies in the preceding steps have been developed and verified, efficacy of phage for controlling microbes in the laboratory models will be determined. Phage applied will be those provided by the Institute of Phage Technology that have been shown in batch testing to be effective against the model bacterial strains. A range of phage application methodologies will be tested to determine the most effective application. In addition to gross control, particular attention will be paid to whether phage can control bacteria under conditions and/or physical locations where traditional biocides were ineffective.
- The final steps of laboratory experimentation will be determination of efficacy of phage to control bacteria in fluids provided by sponsor companies. An important pat of this step will be determining application method(s) that provide the most effective control.
- Required laboratory support for field trials will be provided as needed on an ongoing basis.
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